Method for culturing marine green alga accumulated starch with carbon dioxide

A technology of marine green algae and green algae, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of high starch production costs and reduce production costs, and achieve good sustainability, Environmentally friendly, realize the effect of CO2 emission reduction and recycling

Inactive Publication Date: 2012-05-16
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

In addition, most of the current microalgae culture methods that can accumulate starch are heterotrophic or mixed cultures that add organic matter, which makes the production cost of starch higher, and the use of CO 2 , especially the use of CO in industrial waste gases such as ethanol fermentation tail gas or flue gas 2 Accumulating microalgae starch as the sole carbon source will significantly reduce production costs

Method used

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  • Method for culturing marine green alga accumulated starch with carbon dioxide
  • Method for culturing marine green alga accumulated starch with carbon dioxide
  • Method for culturing marine green alga accumulated starch with carbon dioxide

Examples

Experimental program
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Effect test

Embodiment 1

[0031] The effect of light-dark cycle on algal cell growth and starch accumulation was investigated. Tetraselmis subcordiformis was provided by Dalian Fisheries Research Institute of Liaoning Province, and was purified by plate in our laboratory.

[0032] 1) Obtaining algae cell seed solution

[0033] Preparation of nutrient salt mother solution: prepare 1000 times nutrient salt mother solution with deionized water, the formula is: NaNO 3 100g / L, NaH 2 PO 4 2H 2 O 20.0g / L, EDTA-Na 2 45.0g / L,H 3 BO 3 33.6g / L, MnCl 2 4H 2 O 0.36g / L, FeCl 3 ·6H 2 O 1.3g / L, ZnCl 2 0.21g / L, CoCl 2 ·6H 2 O 0.20g / L, (NH 4 ) 4 Mo 7 o 24 4H 2 O 0.09g / L, CuSO 4 ·5H 2 O 0.20g / L. Sterilize by high pressure steam at 110°C for 15 minutes, and cool down for later use.

[0034] Preparation of algae culture medium: natural seawater was filtered through a microfiltration membrane with a pore size of 0.3 μm. Add 1mL of the above nutrient salt mother solution to every liter of filtered ...

Embodiment 2

[0044] Investigate the initial KNO 3 Concentration effects on algal cell growth and starch accumulation. Tetraselmis subcordiformis was provided by Dalian Fisheries Research Institute of Liaoning Province, and was purified by plate in our laboratory.

[0045] The preparation of the algae cell seed liquid and the preparation of the nitrogen-free nutrient salt mother liquid are the same as in "Example 1".

[0046] Preparation of culture medium: Artificial seawater was filtered through a microfiltration membrane with a pore size of 0.3 μm. The artificial seawater formula is: NaCl 27g / L, MgSO 4 ·7H 2 O 6.6g / L, MgCl 2 ·6H 2 O 5.6g / L, CaCl 2 2H 2 O 1.5g / L, NaHCO 3 0.04g / L. Add 1mL of nitrogen-free nutrient salt mother solution to every liter of filtered seawater. Sterilize by high pressure steam at 110°C for 15 minutes, and cool down for later use.

[0047] Cultivation of algae cells: The sterilized reactor is soaked in NaClO, cleaned with sterile water, and inserted int...

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Abstract

The invention discloses a method for culturing marine green alga accumulated starch with CO2 and marine green alga culturing accumulated starch, and particularly discloses a method for culturing marine green algae and accumulating starch in an illumination bioreactor in a culturing way of using CO2 in gas and adding a nutritional salt. The method comprises the following steps of: adding a nutritional salt into seawater under the condition that marine green alga tetraselmis subcordiformis is taken as an experimental material and CO2 in gas is used in an airlift flat plate illumination reactor; controlling the KNO3 concentration in a culture medium at 0.4-0.6 g/L and continuously illuminating; and culturing for 4-6 days and harvesting alga cells entering a stabilization period, wherein the starch content is 45-55 percent of the dry weight of algae, and the starch yield is 0.25-0.31 g.L<-1>.d<-1>. The method for culturing marine green algae has the advantages of low cost, quick starch accumulation, capability of realizing reduction in emission of CO2, and the like.

Description

technical field [0001] The present invention relates to the cultivation and accumulation of starch of marine green algae, specifically a method of utilizing carbon dioxide (CO2) in the gas 2 ) (including CO in factory exhaust 2 ) and the cultivation method of adding nutrient salts. In the light bioreactor, adopt suitable light, temperature, pH and aeration conditions to cultivate seawater green algae and accumulate starch. Available for CO 2 Emission reduction and recycling, algae cultivation, starch production and energy, food, medicine and other related fields using starch as raw material. Background technique [0002] Human beings are currently facing increasingly serious environmental problems. Large-scale industrial development has brought about excessive emissions of carbon dioxide. by CO 2 The resulting greenhouse effect and global climate change are gradually threatening human survival and development. CO 2 The emission reduction has become the focus of attent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12B01D53/84B01D53/62C12R1/89
CPCY02A50/20Y02P20/59
Inventor 薛松姚长洪陈兆安张卫陆洪斌郑阳
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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