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Method for bio-hydrogen production

A technology for biological hydrogen production and biomass, applied in the direction of fermentation, can solve the problems of low hydrogen production rate, difficult to decompose, and reduce the rate, and achieve the effect of easy operation control and convenient use

Inactive Publication Date: 2012-05-30
SHANGHAI UNIV OF ENG SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current treatment methods of straw-based lignocellulose are not only difficult to decompose, but also have a low hydrogen production rate. Many methods directly combine the process of decomposing lignin and biological hydrogen production, which reduces the rate of each reaction , which is not conducive to rapid and high-volume hydrogen production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for biological hydrogen production, comprising the following process steps:

[0021] (1) Pretreatment of cellulose: crush the cellulose substrate through a 60-mesh sieve, use 1wt% sulfuric acid to hydrolyze at 121°C for 1h under the condition that the solid-to-liquid ratio is 1:8, and then use 5mol / L potassium hydroxide to and.

[0022] (2) Cultivation of Klebsiella propioniformis: Inoculate the bacteria on a specific agar medium, separate and purify.

[0023] (3) Inoculation of Klebsiella propioniformis: the treated cellulose was placed in No. 1 CSTR biohydrogen production reactor with an effective volume of 19 L, water was added until the entire container was covered, and the temperature was adjusted to 35°C. The pH value is 7.0, the stirring speed is 180rpm, and the isolated and purified Klebsiella propioniformis is inoculated. During the process, a certain amount of hydrogen gas will be generated due to the continuation of the reaction, which will be coll...

Embodiment 2

[0027] A method for biological hydrogen production, comprising the steps of:

[0028] (1) Cellulose pretreatment: Grind the straw-like biomass with a mixer, and after passing through a 60-mesh sieve, mix sulfuric acid with a concentration of 1 wt% and the sieved straw-like biomass at a solid-to-liquid ratio of 1:6 , control the temperature at 121 ° C for 1 hour, and then neutralize it with potassium hydroxide with a concentration of 5 mol / L to prepare a reaction solution;

[0029] (2) Inoculation of Klebsiella propionici: the reaction solution obtained in step (1) is placed in a continuous flow tank type stirred reactor (CSTR), and the effective volume of the CSTR is 19L, and the reactor is filled with water , and then put Klebsiella propioniformis in the reactor, and connect the automatic digital display temperature controller to control the reaction temperature to be 34°C, the pH value to be 9.0, and the stirring speed to be 160rpm by adding a resistance wire on the outer wa...

Embodiment 3

[0032] A method for biological hydrogen production, comprising the steps of:

[0033] (1) Cellulose pretreatment: Grind the straw-like biomass with a mixer, and after passing through a 60-mesh sieve, mix sulfuric acid with a concentration of 1 wt% and the sieved straw-like biomass at a solid-to-liquid ratio of 1:10 , control the temperature at 121 ° C for 1 hour, and then neutralize it with potassium hydroxide with a concentration of 5 mol / L to prepare a reaction solution;

[0034] (2) Inoculation of Klebsiella propionici: the reaction solution obtained in step (1) is placed in a continuous flow tank type stirred reactor (CSTR), and the effective volume of the CSTR is 19L, and the reactor is filled with water , then put Klebsiella propioniformis in the reactor, and connect the automatic digital display temperature controller to control the reaction temperature to be 36°C, the pH value to be 3.0, and the stirring speed to be 200rpm by adding a resistance wire on the outer wall ...

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Abstract

The invention relates to a method for bio-hydrogen production, which comprises the following steps: preparing a reaction solution through cellulose pretreatment, putting the reaction solution in a continuous flow tank stirring reactor, adding klebsiella oxytoca in the reactor, degrading the cellulose, adding the product obtained in the degradation into the continuous flow tank stirring reactor for domestication so as to obtain hydrogen producing bacteria, allowing the hydrogen producing bacteria to degrade micromolecular saccharides in the reactor to obtain hydrogen. Compared with the prior art, the invention separates the degradation of cellulose by klebsiella oxytoca from the hydrogen production process, and the cellulose decomposition rate is up to 60%-70%; the specific hydrogen production rate is up to 7.29 mL H2 / g-CDWh, and the hydrogen production speed is up to 25.4 L / d; the operation control is easy, and the application is convenient.

Description

technical field [0001] The invention relates to a method for preparing hydrogen, in particular to a method for biological hydrogen production. Background technique [0002] The use of microorganisms to ferment biomass (organic wastewater, straw-like agricultural and forestry waste, organic waste, etc.) to produce hydrogen has been widely used due to its advantages of environmental protection, no pollution, and comprehensive utilization of waste. , it is of practical significance to realize the sustainable development of resources, energy, environment and economy. However, the current treatment methods of straw-based lignocellulose are not only difficult to decompose, but also have a low hydrogen production rate. Many methods directly combine the process of decomposing lignin and biological hydrogen production, which reduces the rate of each reaction , which is not conducive to rapid and high-volume hydrogen production. Contents of the invention [0003] The object of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00
Inventor 李永峰郜爽张璐婷王元盛
Owner SHANGHAI UNIV OF ENG SCI