Cross-linked hyaluronic acid and hydroxypropyl methyl cellulose composite hydrogel and its preparation method

A technology of hydroxypropyl methylcellulose and cross-linked hyaluronic acid, which is applied in the field of cross-linked hyaluronic acid and hydroxypropyl methylcellulose combined hydrogel and its preparation, can solve the problem of loss of effectiveness and non-cross-linked Hyaluronic acid retention time limit and other issues

Active Publication Date: 2012-06-13
IMEIK TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this way, the product will quickly lose its effectiveness at the injection site (within a week), and as a result, the residence time of non-cro...

Method used

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  • Cross-linked hyaluronic acid and hydroxypropyl methyl cellulose composite hydrogel and its preparation method
  • Cross-linked hyaluronic acid and hydroxypropyl methyl cellulose composite hydrogel and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Preparation of cross-linked hyaluronic acid and hydroxypropyl methylcellulose combined hydrogel

[0023] Dissolve 0.5g of sodium hydroxide in 20ml of water, add 5g of sodium hyaluronate (molecular weight 2.6 million, produced by Shandong Freda Biotechnology Co., Ltd.), stir for 12-14 hours, stir until the sodium hyaluronate is completely dissolved, and then add Add 0.25g 1,2,7,8-diepoxyoctane (also can adopt 1,4-butanediol diglycidyl ether as cross-linking agent, prepare in the same way) in the reaction system, stir rapidly, in 25 °C for 24 hours. The reaction was terminated with 2mol / L hydrochloric acid, and the pH was adjusted to about 5. At the same time, the water in the reaction system was distilled off at 40°C and a vacuum of 13.3Kpa. When the distilled water reached 50mL, the vacuum distillation was stopped. The hydrochloric acid of the gel was neutralized by soaking in 200 mL of sodium hydroxide solution containing 30% ethanol at pH=8-9 for 3 times, ...

Embodiment 2

[0025] Example 2: The cross-linked hyaluronic acid combined hydrogel mixed with hydroxypropyl methylcellulose (HPMC) and the simple cross-linked hyaluronic acid hydrogel (prepared in Example 1, concentration 20mg / ml, theoretical cross-linking degree 5%) enzymatic hydrolysis experiment control

[0026] Take 2 mL of the cross-linked hyaluronic acid hydrogel mixed with hydroxypropyl methylcellulose (HPMC) and the simple cross-linked hyaluronic acid hydrogel prepared in Example 1 in a colorimetric tube, add hyaluronidase 600 Unit, add 2 mL of water to dilute, place in a constant temperature water bath shaker at 37°C, start timing from the 20th minute after dilution, take 50 μL of supernatant with a micro syringe every 20 minutes, and place the taken out supernatant in the refrigerator Rapidly cool to 5°C, take the enzymatic hydrolysis supernatant within 5 hours, and detect the molecular weight of the supernatant at different time intervals by aqueous gel permeation chromatography ...

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Abstract

The invention relates to a cross-linked hyaluronic acid and hydroxypropyl methyl cellulose composite hydroge and its preparation method. Raw materials for the hydrogel comprise, by mass, 1% to 3% of cross-linked hyaluronic acid and 0.5% to 1% of hydroxypropyl methylcellulose , with the balance being water. A method of mixing hydroxypropyl methylcellulose (HPMC) with cross-linked hyaluronic acid especially refers to a high-speed dispersion method. According to the invention, HPMC and hyaluronic acid are simply physically mixed, a chemical reaction does not exist, and after hyaluronic acid is completely degraded, HPMC can be discharged with body fluid, having no potential security risks. The method provided in the invention has the advantages of simpleness, easy operation, low cost, safety and reliability.

Description

technical field [0001] The invention relates to a hydrogel combined with cross-linked hyaluronic acid and hydroxypropyl methylcellulose and a preparation method thereof, that is, a method of mixing hydroxypropyl methylcellulose (HPMC) into cross-linked hyaluronic acid, in particular to The most important is to mix hydroxypropyl methylcellulose (HPMC) in cross-linked hyaluronic acid by high-speed dispersion method. Background technique [0002] To develop dermal fillers that can be used clinically, a series of chemical modifications and treatments must be performed on hyaluronic acid. If non-cross-linked hyaluronic acid is used as a dermal filler, the hyaluronidase and free radicals naturally present in the skin will cut off the polymerized hyaluronic acid molecules one by one, rapidly degrading the non-cross-linked hyaluronic acid. uric acid. As a result, the half-life of hyaluronic acid in the tissue is only one to two days, and then it is diluted with water and gradually...

Claims

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Application Information

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IPC IPC(8): C08L5/08C08L1/28C08J3/075C08J3/24
Inventor 简军李睿智
Owner IMEIK TECH DEV CO LTD
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