In-situ microorganism restoring method for nitrophenol isomeride polluted environment and application
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A technology of isomers and bioremediation, which is applied in the field of environmental organic pollutant treatment, can solve the problems of bioremediation without the three isomers of nitrophenol
Inactive Publication Date: 2012-07-04
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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There are some corresponding reports on the bioremediation of p-nitrophenol (PNP) in soil, but there are no relevant reports on the soil in-situ bioremediation of ortho-nitrophenol (ONP) and m-nitrophenol (MNP), let alone related reports. Simultaneous degradation of three nitrophenol isomers in soil bioremediation reported
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Embodiment 1
[0063] A method for utilizing microorganisms to carry out bioremediation of organic pollutants, the steps of which are:
[0064] A. Construction of microbial mixed flora:
[0065] Three kinds of microbial strains are cultured respectively, the preparation method of Alcaligenes sp.NyZ215 (CCTCC: preservation number M206121), Cupriavidus necator JMP134 (DSMZ: preservation number DSMZ4058), Pseudomonas sp.WBC-3 (CCTCC: preservation number M201027), comprises the following steps :
[0066] a. Preparation of inorganic salt medium: the composition is disodium hydrogen phosphate (Na 2 HPO 4 12H 2 O) 14.3g, potassium dihydrogen phosphate (KH 2 PO 4 ) 3.0g, magnesium sulfate (MnSO 4 ·H 2 O) 0.28mg, ferrous sulfate (FeSO 4 ·7H 2 O) 0.3mg, magnesium sulfate (MgSO 4 ) 0.06mg, calcium chloride (CaCl 2 ) 1mg, copper sulfate (CuSO 4 ) 0.05mg, zinc sulfate (ZnSO 4 ) 0.05mg and boric acid (H 3 BO 3 ) 0.05mg, dilute it to 1000ml with double distilled water, adjust the pH to 7.0, ...
Embodiment 2
[0083] A kind of application utilizing microbial degrading flora in nitrophenol in-situ bioremediation (environmental pollution control), its steps are:
[0084] A. Construction of microbial mixed flora:
[0085] Three kinds of microbial strains are cultured respectively, the preparation method of Alcaligenes sp.NyZ215 (CCTCC: preservation number M206121), Cupriavidus necator JMP134 (DSMZ: preservation number DSMZ4058), Pseudomonas sp.WBC-3 (CCTCC: preservation number M201027), comprises the following steps :
[0086] a. Preparation of inorganic salt medium: the composition is disodium hydrogen phosphate (Na 2 HPO 4 12H 2 O) 14.3g, potassium dihydrogen phosphate (KH 2 PO 4 ) 3.0g, magnesium sulfate (MnSO 4 ·H 2 O) 0.28mg, ferrous sulfate (FeSO 4 ·7H 2 O) 0.3mg, magnesium sulfate (MgSO 4 ) 0.06mg, calcium chloride (CaCl 2 ) 1mg, copper sulfate (CuSO 4 ) 0.05mg, zinc sulfate (ZnSO 4 ) 0.05mg and boric acid (H 3 BO 3 ) 0.05mg, dilute it to 1000ml with double distil...
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Abstract
The invention discloses an in-situ restoring method for a nitrophenol isomeride polluted environment by utilizing a microorganism mixed flora and an application. The in-situ restoring method comprises the following steps: A) constructing the microorganism mixed flora: a) preparing an inorganic salt culture medium, b) performing induction culture on strains and c) collecting thallus; B) constructing different micro environmental models: a) constructing a soil micro environmental model and b) preparing a biological reaction vessel; and C) placing a soil sample at 30 DEG C, placing the biological reaction vessel at room temperature, and sampling and detecting the change in pollutant concentration at regular time. According to the method provided by the invention, the in-situ restoring of thesoil containing 40ppm per milligram dry weight nitrophenol can be realized; the biological restoring for three nitrophenols in the 50 milligram per liter biological reaction vessel can be realized; multiple inoculated bacteria can continuously and stably exist in the soil and the reaction vessel, thereby proving that the in-situ restoring method can be applied to the environmental treatment of soil and water containing nitrophenol isomeride pollution; and a new idea for treating the environmental pollutant is supplied.
Description
technical field [0001] The invention relates to the technical field of environmental organic pollutant treatment, in particular to a method for bioremediation of organic pollutants by using microorganisms. More specifically, it involves the use of three strains of Alcaligenes sp.NyZ215, Cupriavidus necator JMP134 and Pseudomonas sp.WBC-3 to form a mixed flora for in situ bioremediation in environments contaminated by three nitrophenol isomers, while also involving microorganisms The use of degrading bacteria in in situ bioremediation of nitrophenol isomers achieves the purpose of effectively removing pollutants. Background technique [0002] Nitrophenols (nitrophenols) belong to nitro aromatic hydrocarbon compounds, including 3 isomers: 4-nitrophenol (p-nitrophenol, p-nitrophenol, PNP), 3-nitrophenol (m-nitrophenol Phenol, m-nitrophenol, MNP) and 2-nitrophenol (o-nitrophenol, o-nitrophenol, ONP), they are all important chemical raw materials, often used as synthetic precurs...
Claims
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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10C02F3/34
Inventor 周宁一迟向群傅贺张俊杰刘虹王淑君
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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