Bacillus simplex, and culture method and application thereof

A technology of simple bacillus and a culture method, applied to simple bacillus and its culture method and application field, can solve problems such as blanks, and achieve the effects of expanded production, rapid growth and reproduction, and simple and easy-to-operate culture method

Inactive Publication Date: 2013-10-16
RES INST OF SHAANXI YANCHANG PETROLEUM GRP
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many cases of using other bacillus to degrade crude oil, but the research on using simple bacillus to degrade crude oil is still blank

Method used

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  • Bacillus simplex, and culture method and application thereof
  • Bacillus simplex, and culture method and application thereof
  • Bacillus simplex, and culture method and application thereof

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0018] The present invention will be described in detail below in combination with specific embodiments.

[0019] The Bacillus simplex YM8 involved in the present invention has been deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on September 9, 2011, and the preservation number is CGMCC No.5242.

[0020] The experimental methods in the following examples, unless otherwise specified, are conventional methods; the experimental materials used in the following examples, unless otherwise specified, are conventional biochemical reagents.

[0021] The media involved in the following examples are: peptone, beef extract, NaCl, agar, glucose, corn steeple, sodium nitrate, disodium hydrogen phosphate, potassium dihydrogen phosphate, calcium chloride, magnesium sulfate, cedar oil, xylene , crystal violet, ammonium oxalate.

[0022] 1. Isolation, purification, screening and cultivation of Bacillus simplex YM8 CGMCC No.5242:

[0023] ...

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Abstract

The invention relates to a bacillus simplex YM8 CGMCC No.5242, and a culture method and application thereof. The culture method for the bacillus simplex YM8 comprises the following steps: inoculating the strain into a seed culture medium; cultivating in a shaking table at the temperature of 35 or 55 DEG C and at the rotating speed of 120 rpm until the strain concentration reaches 10<8> to 10<9>/mL; inoculating 5 mL of bacterial liquid into a culture medium; and cultivating in the shaking table at the temperature of 35 or 55 DEG C and at the rotating speed of 120 rpm. The bacillus simplex YM8 can be applied to environmental management on the oil field, and has a good effect of degrading crude oil, and higher research, application and market values. The culture method is simple and easy to operate, quick in growth and propagation and practicable in expanding production.

Description

technical field [0001] The invention relates to a strain of bacteria and its cultivation method and application, in particular to a strain of Bacillus simplex and its cultivation method and application. Background technique [0002] Pollution often occurs in the process of oil extraction, transportation, processing and utilization, even large-scale pollution. The use of bioremediation technology (also known as bioremediation, biological restoration, ecological restoration or ecological restoration refers to the process of using the metabolic activities of organisms in the treatment system, mainly microorganisms, to reduce the concentration of pollutants at the polluted site or make them harmless. ) can be widely used in the treatment of crude oil pollution environment. Microbial treatment of crude oil pollution is the degradation of some refractory crude oil components by microorganisms, which is completed through the catalysis of a series of oxidases. In nature, this proc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K8/524C09K3/32C12R1/07
Inventor 王香增高瑞民李世强江绍静洪玲赵丽薛媛王蓓蕾
Owner RES INST OF SHAANXI YANCHANG PETROLEUM GRP
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