Strain culture solution for preventing root knot nematode and preparation method thereof

Abstract

The invention relates to a biological agent and a preparation method thereof, and in particular relates to a strain culture solution for preventing root knot nematode and a preparation method thereof. The invention provides an environment-friendly strain culture solution for preventing root knot nematode with a good prevention effect and a preparation method thereof. The main composition of the strain culture solution is the strain named as Photorhabdustemperata.subsp.Temperate. The Heterorhabditis bacteriophora is sequentially subjected to crushing, separation, inoculation culture and extraction to obtain the strain culture solution. Through the invention, the existing chemical pesticide preparation is replaced; the soil damaged by residual pesticide can be reduced; and the harm of the root knot nematode can be reduced by adopting the culture solution containing the component since the native strain can generate a substance capable of killing nematode.

Description

Technical field: [0001] The invention relates to a biological agent and a preparation method thereof, more specifically, to a bacterial strain culture solution for preventing and controlling root-knot nematodes and a preparation method thereof. Background technique: [0002] The plant parasitic nematode that damages crops widely distributed in the world is root-knot nematode, which damages the above-ground parts of plants or injures the roots of plants while inhabiting in the soil, especially in China, sweet potato root-knot nematode (Meloidogyne incognita), Peanut root-knot nematode (Meloidogyne arenaria), carrot root-knot nematode (Meloidogyne hapla) etc. are well known as the main pests causing damage to crops. [0003] At present, root-knot nematode control methods include cultivation control, physical control and chemical control. Cultivation control includes fresh water, fallow, dry soil, deep plowing, foreign soil, rice rotation cultivation, disease-resistant varieti...

AI Technical Summary

Problems solved by technology

Cultivation control has the disadvantages of difficult application and high cost depending on the regional conditions and cultivated crops, especially the disease-resistant varieties have limitations on crops, so the practicability is very low

In the case of chemical agents, the control effect is high, but most of them are organic phosphorus agents and carbamates. When there are residues in the soil, they are highly toxic, which not only pollutes the environment, but also has no difference to the beneficial microorganisms in the soil on which crops depend. play a bactericidal effect, making the pest intensified, which is the reason for the soil becoming barren

[0004] As an alternative to chemical control agents, this biological control using natural enemy microorganisms, biochemical agents and plant extracts of root-knot nematodes is actively being studied, and up to now, reported insecticidal fungi ( Monacroporium thaumasium) is extremely ineffective in the actual facility cultivation field, and it is also difficult to popularize the practical application to farmers. In addition, promising research on the use of medicinal plants to achieve nematicidal effects is also in progress, and the collection of raw materials It is difficult, and the disadvantage is that it still needs to rely on imports

Images