CsCoL1 gene relative to cymbidium sinense photoperiod and application of CsCoL1 gene

A photoperiod and gene technology, applied in the fields of molecular biology and genetic engineering, to achieve great economic value, broad application prospects, and shorten the growth period.

Inactive Publication Date: 2012-07-11
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • CsCoL1 gene relative to cymbidium sinense photoperiod and application of CsCoL1 gene
  • CsCoL1 gene relative to cymbidium sinense photoperiod and application of CsCoL1 gene
  • CsCoL1 gene relative to cymbidium sinense photoperiod and application of CsCoL1 gene

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Embodiment 1

[0024] 1. Cloning and homology analysis of the CsCOL1 gene of Molan.

[0025] (1) The Molan variety "Cymbidium sinense.'Qi Jian Bai Mo') was used as the experimental material, and the plant material was grown under normal conditions in the greenhouse (L / D, 14h / 10h; 25-28°C).

[0026] (2) RNA extraction: Trizol reagent (purchased from Invitrogen) was used to extract the total RNA of the test materials, and the whole operation process was strictly in accordance with the RNA extraction process description of the Trizol reagent.

[0027] (3) M-MLV reverse transcriptase (purchased from Promgra Company) was used to reverse transcribe the mRNA into the first strand of cDNA, and the method was carried out according to the reagent instructions.

[0028]Gene cloning: using the reverse-transcribed first-strand cDNA of orchid flower buds as a template, two forward-specific primers CO-3A and CO-3B were designed according to the conserved sequence of CONSTANS-like homologous genes, and ampl...

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Abstract

The invention discloses a CsCoL1 gene relative to cymbidium sinense photoperiod and the application of the CsCoL1 gene. The nucleotide sequence of the CsCoL1 gene is shown from 40th site base to 1020th site base in SEQ ID (sequence identity) NO.1; the nucleotide sequence of encoding protein of the CsCoL1 gene is shown in SEQ ID NO.2; a recombinant plant expression vector contains the CsCoL1 gene;in addition, the CsCoL1 gene is applied to shortening the juvenile period of plants and promoting the plants to bloom. The CsCoL1 gene is cloned from a CONSTANS-like gene-CsCoL1 gene in cymbidium sinense, can be proved to influence the growth and development program of the plants through experiments, and can promote the plants to bloom. Therefore, the CsCoL1 gene of cymbidium sinense provides an effective technical means to improve the growth program of the plants in gene engineering as well as adjust and control the blooming period of the plants, and achieves a broad application prospect anda great economic value.

Description

Technical field: [0001] The invention belongs to the technical fields of molecular biology and genetic engineering, and specifically relates to a photoperiod-related CONSTANS-like gene-CsCOL1 gene and its encoded protein, a recombinant plant expression vector containing the gene, and CsCOL1 expressed in Molan Application of genes in promoting plant growth and development. Background technique: [0002] Day length (i.e. photoperiod) is one of the main factors affecting flowering time. Arabidopsis is a typical long-day plant, long-day can promote Arabidopsis flowering, while short-day can inhibit flowering. The current study found that the main regulatory genes in the photoperiod pathway are CONSTANS (CO), CRY2 / FHA, GIGANTEA (GI), FT and FWA, and their mutants delayed flowering under long-day (LD) conditions, but in short The flowering time under daylight (SD) conditions was similar to that of the wild type. [0003] CO is a key gene in the plant photoperiod signal transduc...

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82C12N15/84A01H5/00
Inventor 张建霞曾宋君田瑞雪段俊吴坤林
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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