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A production method and technology of cordycepin, applied in the field of fermentation production of cordycepin, to achieve the effect of facilitating migration, convenient operation and high yield
Active Publication Date: 2012-07-11
JIANGSU FANGSHIYUANLVE SCI & TECH CONSULTING CO LTD
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Abstract
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Problems solved by technology
The slow growth of strains in this process leads to long fermentation time, low fermentation yield and low concentration of fermentation products
Method used
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[0028] Preservation medium and seed medium are prepared as follows: take 200 grams of peeled potatoes, cut them into 1 cm square pieces, add 1000 ml of water and boil for 30 minutes, filter the potato pieces with 4 layers of gauze, make up the filtrate to 1000 ml, add Glucose 20g, KH 2 PO 4 3 g, MgSO 4 . 7H 2 O 1.5g, vitamin B1 0.2g, agar 15g, after melting, aliquot and sterilize at 121°C for 30 minutes.
[0029] The formula of the fermentation medium is as follows: glucose 42g / L, yeast extract 6g / L, peptone 10g / L, KH 2 PO 4 0.5g / L, K 2 HPO 4 ·3H 2 O 0.5g / L, MgSO 4 ·7H 2 O 0.5g / L, the solvent is water, pH 6.0, sterilized at 121°C for 20 minutes.
[0030] Strain preservation: the strains used are preserved on the slant of the preservation medium, stored at 4°C, and transferred once every two months.
[0036] Preservation medium and seed medium are prepared as follows: take 200 grams of peeled potatoes, cut them into 1 cm square pieces, add 1000 ml of water and boil for 30 minutes, filter the potato pieces with 4 layers of gauze, make up the filtrate to 1000 ml, add Glucose 20g, KH 2 PO 4 3 g, MgSO 4 . 7H 2 O 1.5g, vitamin B1 0.2g, agar 15g, after melting, aliquot and sterilize at 121°C for 30 minutes.
[0037] The formula of the fermentation medium is as follows: glucose 42g / L, yeast extract 6g / L, peptone 10g / L, KH 2 PO 4 0.5g / L, K 2 HPO 4 ·3H 2 O 0.5g / L, MgSO 4 ·7H 2 O 0.5g / L, the solvent is water, pH 6.0, sterilized at 121°C for 20 minutes.
[0038] Strain preservation: the strains used are preserved on the slant of the preservation medium, stored at 4°C, and transferred once every two months.
[0039] Seed culture: transfer from the...
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Abstract
The invention discloses a fermenting production method of cordycepin, and the method comprises the following steps of: transplanting activated bacterial strain cordyceps militaris into a fermentation tank filled with a fermentation culture medium, wherein the fermentation temperature is 25-28 DEG C, and the stirring rotation speed is 150-180rpm (revolutions per minute); feeding sodium hydroxide solution and hydrochloric acid to control the pH value of fermentation liquor at 5.5-6.0; and when a carbon source is completely consumed, reducing the stirring rotation speed to be 60-80 rpm, and replenishing a water solution containing glucose and adenosine into the fermentation tank by means of intermittent feeding, wherein the whole fermentation time is 200-280 hours. According to the fermenting production method, the pH value of the fermentation process is controlled, and the energy sources and the cordycepin precursor are timely replenished, so that the fermentation process can be kept under an optimum environment, and the fermenting production method is good for the growth of thallus, and good for the transplantation of metabolic flux, therefore, the cordycepin yield can be finally up to 10.0g / L, the production efficiency reaches 0.25g / L / d, and the method is simple in process, lower in environment pollution, convenient to operate, and high in cordycepin yield.
Description
technical field [0001] The invention relates to a fermentation production method of cordycepin, which belongs to the technical field of biological fermentation engineering. Background technique [0002] Cordycepin, namely 3'-deoxyadenosine. It has a variety of biological activities, such as anti-tumor, anti-proliferation, anti-metastasis, antibacterial, antiviral, immunomodulatory and anti-inflammatory, etc. In the last decade, great progress has been made in the targeted research of cordycepin as a therapeutic drug. These studies include the application of cordycepin in various cancers, especially leukemia (it has entered phase III clinical trials in the United States), as well as restenosis induced by African trypanosomiasis and angioplasty, and in vivo anti-oxidation of cordycepin derivatives effect etc. Therefore, the synthesis technology of cordycepin is a research subject that people are more concerned about. [0003] In countries such as Japan and South Korea, t...
Claims
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Application Information
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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/40C12R1/645
Inventor 汤佳鹏葛彦朱俐姜正林
Owner JIANGSU FANGSHIYUANLVE SCI & TECH CONSULTING CO LTD
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