The invention discloses a fermenting production method of 
cordycepin, and the method comprises the following steps of: 
transplanting activated 
bacterial strain cordyceps militaris into a 
fermentation tank filled with a 
fermentation culture medium, wherein the 
fermentation temperature is 25-28 DEG C, and the stirring rotation speed is 150-180rpm (
revolutions per minute); feeding 
sodium hydroxide solution and 
hydrochloric acid to control the pH value of fermentation liquor at 5.5-6.0; and when a 
carbon source is completely consumed, reducing the stirring rotation speed to be 60-80 rpm, and replenishing a water solution containing glucose and 
adenosine into the fermentation tank by means of intermittent feeding, wherein the whole fermentation time is 200-280 hours. According to the fermenting production method, the pH value of the fermentation process is controlled, and the energy sources and the 
cordycepin precursor are timely replenished, so that the fermentation process can be kept under an optimum environment, and the fermenting production method is good for the growth of 
thallus, and good for the 
transplantation of metabolic flux, therefore, the 
cordycepin yield can be finally up to 10.0g / L, the production efficiency reaches 0.25g / L / d, and the method is simple in process, lower in environment 
pollution, convenient to operate, and high in cordycepin yield.