Rapid propagation method for Miscanthus floridulus

A five-jointed, fast technology, applied in the field of plant cell engineering, can solve problems that have not been reported, and achieve the effect of reducing the healing rate, avoiding browning of young ears, and ensuring genetic stability

Inactive Publication Date: 2012-07-18
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Diao Ying et al. In a method for rapid propagation of somatic embryo tissue culture of Miscanth quinquefolius, young ears are used as explants to induce embryogenic callus. The length of youn

Method used

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  • Rapid propagation method for Miscanthus floridulus
  • Rapid propagation method for Miscanthus floridulus
  • Rapid propagation method for Miscanthus floridulus

Examples

Experimental program
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Embodiment 1

[0021] Materials: M.floridulus 02117 was collected from Chenzhou, Hunan, and preserved in the Miscanth plant resource nursery of Hunan Agricultural University.

[0022] 1. Selection and sterilization of explants: select excellent single plants of Miscanthus chinensis, and cut out young panicles in the formation stage of spikelet primordia with a leaf-to-occipital distance of 0. First, remove the outer leaves of the young panicles, leaving only the flag leaves, wash them with sterile water for 3 times, then treat them with 70% alcohol on the aseptic operating table for about 15 seconds, and then sterilize them with 0.1% mercuric chloride for 18 minutes. Rinse with sterile water 6 times for later use;

[0023] 2. Induction of embryogenic callus: remove the flag leaves from the sterilized young ears with flag leaves on the ultra-clean bench, cut the young ears into ear segments of about 0.3 cm, and inoculate them in callus induction culture Base: MS+2,4-D2.0mg / L+6-BA0.1mg / L. In...

Embodiment 2

[0027] Material: M.floridulus 04405 was collected from Ma'anshan, Anhui, and preserved in the Miscanth plant resource garden of Hunan Agricultural University.

[0028] 1. Selection and sterilization of explants: select excellent single plants of Miscanthus chinensis, and cut young ears with a leaf-occipital distance of 1.5 cm. First, remove the outer leaves of the young panicle, leaving only the flag leaf, wash it with sterile water for 4 times, treat it with 70% alcohol for about 20 seconds on a sterile operating table, and then disinfect it with 0.1% mercuric chloride for 20 minutes. Rinse with sterile water 6 times for later use;

[0029] 2. Induction of embryogenic callus: remove the flag leaves from the sterilized young ears with flag leaves on the ultra-clean bench, cut the young ears into ear segments of about 0.4 cm, and inoculate them in callus induction culture Base: MS+2,4-D4.0mg / L+6-BA0.2mg / L. Inoculate 4 panicle segments in each bottle in the inoculation bottle,...

Embodiment 3

[0033] Materials: M.floridulus 02155 was collected from Changde, Hunan, and preserved in the Miscanth plant resource nursery of Hunan Agricultural University.

[0034] 1. Selection and sterilization of explants: select excellent single plants of Miscanthus chinensis, and cut young panicles in the formation stage of spikelet primordia with a leaf-to-occipital distance of 2 cm. First, clean the outer leaves of the young panicle, leaving only the flag leaf, wash it with sterile water for 4 times, treat it with 70% alcohol for about 30 seconds on the aseptic operating table, and then disinfect it with 0.1% mercuric chloride for 20 minutes. Rinse with sterile water 7 times for later use;

[0035] 2. Induction of embryogenic callus: remove the flag leaves from the sterilized young ears with flag leaves on the ultra-clean bench, cut the young ears into ear segments of about 0.5 cm, and inoculate them in callus induction culture Base: MS+2,4-D6.0mg / L+6-BA0mg / L. Inoculate 3 panicle s...

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Abstract

The invention discloses a rapid propagation method for Miscanthus floridulus, which comprises the steps of: taking young spikes of Miscanthus floridulus as explants, selecting the young spikes of Miscanthus floridulus at a glumous flower primordium forming stage, sterilizing, cutting the young spikes into spike sections, inoculating the spike sections in callus induction culture mediums MS+ 2.0-6.0mg/L and 2, 4-D+ 0-0.2mg/L 6-BA, and culturing under light with light intensity being 250-300lux at 24 DEG C plus or minus 2 DEG C, wherein calluses can be generated within 7 days approximately; inoculating the calluses in differentiation culture medium MS+ 1.0-4.0mg/L 6-BA, and culturing under light with light intensity being 2000lux at 24 DEG C plus or minus 2 DEG C till cluster buds are formed and the reproduction factor is above 4; using MS+ 1.0mg/L, IAA+ 0.1mg/L and IBA+ 1.0mg.L CCC to induce buds and roots, wherein the rooting rate is above 95 percent and complete plants can be formed within only 35 days approximately.

Description

technical field [0001] The invention belongs to the field of plant cell engineering, and relates to plant tissue engineering technology, in particular to a method for rapid tissue propagation of awns. Background technique [0002] Miscanthus floridulus belongs to the Poaceae family Miscanthus Andersson (Chen Shouliang, 1997). It is a high-efficiency C4 plant with high biomass production, strong stress resistance, and high cellulose content. It is considered to be a biomass energy plant with great development potential, and it is mainly distributed in tropical and subtropical regions in my country (Xiao Yunfeng, 1995). At present, there are a few reports on the tissue culture of acanthus chinensis. Hu Hengkang et al. (Plant Physiology Communication, 2009) used seeds as explants, and cut off the radicle of the germinated seedlings to induce clustered shoots. Diao Ying et al. In a method for rapid propagation of somatic embryo tissue culture of Miscanth quinquefolius, young ear...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈智勇易自力覃静萍蒋建雄艾辛
Owner HUNAN AGRICULTURAL UNIV
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