Method for screening antifungal substance acting on glucosylceramide as target

A technology of ceramide glycolipid and ceramide, applied in the field of antifungal substances, can solve the problems of unclear target and toxicity of antifungal substances, large workload, etc., and achieve the effects of accurate and reliable results, simple screening process and clear targets
CN102586394BInactive Publication Date: 2014-01-01INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Publication Date
2014-01-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for screening an antifungal substance acting on glucosylceramide as a target. The method provided by the invention includes the following steps: a wild fungus strain and a mutant strain are respectively cultured on solid plates containing a substance to be assayed, and if the growth of the mutant strain on the plate is better than the growth of the wild fungus strain on the plate, the substance to be assayed is a candidate antifungal substance or a candidate substance containing the antifungal substance; and the target of the antifungal substance is glucosylceramide. The method established by the invention has the advantages that: the target is specific, moreover, the method integrates in vivo screening and activity assay, whether the substance is the antifungal substance targeting glucosylceramide can be judged only according to the difference between the sensitivities of the mutant strain and the wide strain on the antifungal substance, the screening process is simpler, and can save time and labor, and the result is more accurate and reliable. The method has a great application prospect in the screening and development of green control agents for fungal diseases.
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Description

technical field

[0001] The invention relates to a method for screening antifungal substances with ceramide glycolipid as the action target. Background technique

[0002] Plant defensins are broad-spectrum antifungal short peptides ubiquitous in a class of plants, which play a key role in plant defense against pathogenic fungal invasion. Among them, the mechanism of the plant defensin RsAFP2 from carrot is relatively clear at present, and the antibacterial effect of RsAFP2 is realized by combining with ceramide glycolipid (glucosylceramide) in the fungal cell membrane. RsAFP2 is not toxic to humans and plants because RsAFP2 cannot bind to ceramide glycolipids in humans and plants. Therefore, the development of pesticides whose mechanism of action is similar to plant defensin RsAFP2 is an ideal way to achieve green control of plant fungal diseases.

[0003] The large-scale expression of plant defensins by using microorganisms is an ideal model to realize their large-scale ap...

Claims

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