Immune colloidal gold test paper and method for quantitatively detecting configurable logic block (CLB) by matching with photoelectric sensor thereof

A colloidal gold test strip, colloidal gold technology, applied in the direction of color/spectral property measurement, instruments, measuring devices, etc., can solve the problems of large-scale instrument immune colloidal gold test strips that cannot be quantitatively detected, the detection time is long, and the processing is complicated. The effect of good application prospect, fast detection speed and low preparation cost

Inactive Publication Date: 2012-07-18
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims at the defects of the existing clenbuterol hydrochloride sample pretreatment is complicated, the detection time is long, large-scale instruments are required, and tra

Method used

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  • Immune colloidal gold test paper and method for quantitatively detecting configurable logic block (CLB) by matching with photoelectric sensor thereof
  • Immune colloidal gold test paper and method for quantitatively detecting configurable logic block (CLB) by matching with photoelectric sensor thereof
  • Immune colloidal gold test paper and method for quantitatively detecting configurable logic block (CLB) by matching with photoelectric sensor thereof

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Effect test

Embodiment 1

[0038]The preparation of the immune colloidal gold test strip of the present embodiment and the method steps of quantitatively detecting clenbuterol hydrochloride combined with the photoelectric sensor are as follows:

[0039] (1) Preparation of colloidal gold solution

[0040] First, soak the required experimental equipment in acid for 12 hours to prepare a colloidal gold solution. Measure 100 mL of distilled water with a graduated cylinder, add 100 μL of 1% chloroauric acid, and when heated to boiling, add 100 μL of 1% trisodium citrate and heat to the solution When it turns purple, turn off the heat and cook for another 3 minutes.

[0041] (2) Production of colloidal gold protection pad

[0042] Use a clean pipette to draw 10mL of colloidal gold solution into a small beaker, draw 4μL of antibody and add it to 1mL of Tris-HCl buffer solution, add the mixture to the colloidal gold solution while stirring, wait for 10min, Use potassium carbonate to adjust the pH to greater t...

Embodiment 2

[0050] The preparation of the immune colloidal gold test strip of the present embodiment and the method steps of quantitatively detecting clenbuterol hydrochloride combined with the photoelectric sensor are as follows:

[0051] (1) Preparation of colloidal gold solution

[0052] Before preparing immune colloidal gold test strips, soak the required experimental instruments in acid for 15 hours, the first choice is to prepare colloidal gold solution, measure 200 mL of distilled water with a graduated cylinder, add 200 μL of 1% chloroauric acid, and when heated to boiling, add 1 300 μL of trisodium citrate, heated until the solution turns purple, then turn off the low heat and cook for 5 minutes.

[0053] (2) Production of colloidal gold protection pad

[0054] Use a clean pipette to draw 10mL of colloidal gold solution into a small beaker, draw 10μL of antibody and add it to 1mL of Tris-HCl buffer solution, add the mixture to the colloidal gold solution while stirring, wait for...

Embodiment 3

[0062] The preparation of the immune colloidal gold test strip of the present embodiment and the method steps of quantitatively detecting clenbuterol hydrochloride combined with the photoelectric sensor are as follows:

[0063] (1) Preparation of colloidal gold solution

[0064] Before preparing immune colloidal gold test strips, soak the required experimental instruments in acid for 20 hours. The first choice is to prepare colloidal gold solution. Use a measuring cylinder to measure 100 mL of distilled water, add 400 μL of 1% chloroauric acid, and when heated to boiling, add 1 500 μL of trisodium citrate, heated until the solution turns purple, then turn off the heat and cook for another 10 minutes.

[0065] (2) Production of colloidal gold protection pad

[0066] Use a clean pipette to draw 10mL of colloidal gold solution into a small beaker, draw 15μL of antibody and add it to 1mL of Tris-HCl buffer solution, add the mixture to the colloidal gold solution while stirring, w...

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Abstract

The invention discloses immune colloidal gold test paper and a method for quantitatively detecting a configurable logic block (CLB) by matching with a photoelectric sensor thereof. The immune colloidal gold test paper comprises absorbent paper, a nitrocellulose (NC) membrane, a colloidal gold protective pad and a glass fiber which are arranged on a support plate and overlapped partially and mutually in sequence. Two round holes are arranged on the support plate, round areas on the NC membrane corresponding to the two round holes on the support plate are coated by T zone stripe of CLB-bovine serum albumin (BAS) and C zone stripe of a goat- anti-mouse second antibody, and the colloidal gold protective pad comprises an antibody resisting clenbuterol hydrochloride and marked by colloidal gold. The method for quantitatively detecting the CLB includes the following steps: a tested immune colloidal gold test paper strip is placed in a detecting area of the photoelectric sensor to be detected; the photoelectric sensor converts light reflected by the detecting area into electric signals, and concentration of the CLB is calculated according to strength of the electric signals.

Description

technical field [0001] The invention belongs to the technical fields of monoclonal antibody technology, gold-labeled antibody technology, competitive immune chromatography technology and photoelectric sensor, and specifically relates to an immune colloidal gold test paper and a method for quantitatively detecting CLB with a photoelectric sensor. Background technique [0002] Clenbuterol hydrochloride, also known as clenbuterol, is a synthetic β 2 - Adrenergic receptor agonist, which can significantly increase the lean meat rate after animal consumption, but at the same time, it also causes CLB residues in animal foods, thus causing human poisoning events. The World Health Organization and the Food and Agriculture Organization of the United Nations stipulate that the maximum residue limit of CLB in edible animal tissues is 0.01ng / g. The colloidal gold immunochromatography test uses the principle of antigen-antibody reaction, uses the colloidal gold-labeled tracer to form a c...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558G01N21/27
Inventor 柴春彦张洪才尹璐刘国艳
Owner SHANGHAI JIAO TONG UNIV
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