Immune colloidal gold test paper and method for quantitatively detecting configurable logic block (CLB) by matching with photoelectric sensor thereof
A colloidal gold test strip, colloidal gold technology, applied in the direction of color/spectral property measurement, instruments, measuring devices, etc., can solve the problems of large-scale instrument immune colloidal gold test strips that cannot be quantitatively detected, the detection time is long, and the processing is complicated. The effect of good application prospect, fast detection speed and low preparation cost
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Embodiment 1
[0038]The preparation of the immune colloidal gold test strip of the present embodiment and the method steps of quantitatively detecting clenbuterol hydrochloride combined with the photoelectric sensor are as follows:
[0039] (1) Preparation of colloidal gold solution
[0040] First, soak the required experimental equipment in acid for 12 hours to prepare a colloidal gold solution. Measure 100 mL of distilled water with a graduated cylinder, add 100 μL of 1% chloroauric acid, and when heated to boiling, add 100 μL of 1% trisodium citrate and heat to the solution When it turns purple, turn off the heat and cook for another 3 minutes.
[0041] (2) Production of colloidal gold protection pad
[0042] Use a clean pipette to draw 10mL of colloidal gold solution into a small beaker, draw 4μL of antibody and add it to 1mL of Tris-HCl buffer solution, add the mixture to the colloidal gold solution while stirring, wait for 10min, Use potassium carbonate to adjust the pH to greater t...
Embodiment 2
[0050] The preparation of the immune colloidal gold test strip of the present embodiment and the method steps of quantitatively detecting clenbuterol hydrochloride combined with the photoelectric sensor are as follows:
[0051] (1) Preparation of colloidal gold solution
[0052] Before preparing immune colloidal gold test strips, soak the required experimental instruments in acid for 15 hours, the first choice is to prepare colloidal gold solution, measure 200 mL of distilled water with a graduated cylinder, add 200 μL of 1% chloroauric acid, and when heated to boiling, add 1 300 μL of trisodium citrate, heated until the solution turns purple, then turn off the low heat and cook for 5 minutes.
[0053] (2) Production of colloidal gold protection pad
[0054] Use a clean pipette to draw 10mL of colloidal gold solution into a small beaker, draw 10μL of antibody and add it to 1mL of Tris-HCl buffer solution, add the mixture to the colloidal gold solution while stirring, wait for...
Embodiment 3
[0062] The preparation of the immune colloidal gold test strip of the present embodiment and the method steps of quantitatively detecting clenbuterol hydrochloride combined with the photoelectric sensor are as follows:
[0063] (1) Preparation of colloidal gold solution
[0064] Before preparing immune colloidal gold test strips, soak the required experimental instruments in acid for 20 hours. The first choice is to prepare colloidal gold solution. Use a measuring cylinder to measure 100 mL of distilled water, add 400 μL of 1% chloroauric acid, and when heated to boiling, add 1 500 μL of trisodium citrate, heated until the solution turns purple, then turn off the heat and cook for another 10 minutes.
[0065] (2) Production of colloidal gold protection pad
[0066] Use a clean pipette to draw 10mL of colloidal gold solution into a small beaker, draw 15μL of antibody and add it to 1mL of Tris-HCl buffer solution, add the mixture to the colloidal gold solution while stirring, w...
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