Preparation method of pachyman sulfate

Abstract

The invention discloses a preparation method of pachyman sulfate. The preparation method comprises the following steps of: adding sulfamic acid and an organic solvent in a reaction bottle of a rotary evaporator; preheating to reaction temperature; after fully dissolving the sulfamic acid, adding dried pachyman powder; vacuuming a reaction system; cooling to room temperature after the reaction is finished; adjusting the pH of reaction solution to 9-10 with saturated alkali solution; continuously vacuuming to remove ammonia and repeatedly vacuuming until the reaction solution has no ammonia smell; maintaining the pH value to be over 7 until the pH value does not go down; then adding ethanol; precipitating polysaccharide sulfate; filtering and then dissolving in water again; filtering; placing in an ultrafilter to desalinate and concentrate; and detecting residual quantity of free sulfate radicals in eluent by using saturated barium chloride solution until no white precipitate is produced. According to the preparation method, the sulfation efficiency is greatly increased, polysaccharide molecule degradation and production of sugar ring double ring are suppressed, the color of a product becomes light, and the final quality of the product is obviously improved.

Description

technical field

[0001] The invention belongs to the field of medicinal chemistry, and in particular relates to a preparation method of polysaccharide sulfate. Background technique

[0002] Polysaccharides are the most abundant substances in nature and are composed of monosaccharide residues bonded by covalent bonds. Polysaccharide sulfate is a polysaccharide derivative, which is formed by substituting some hydroxyl groups of monosaccharide residues in the polysaccharide molecular chain with sulfate groups. Among them, the molecular weight and the degree of substitution of sulfate groups (DS) of monosaccharide residues are the two most important physical and chemical parameters. . Polysaccharide sulfate has a wide range of pharmacological activities, such as anticoagulant, antithrombotic, antiviral, hypolipidemic and antitumor activities. In 1987, Beyer of the former Federal Republic of Germany cooperated with Hoechst Company to artificially synthesize sulfated xylan for th...

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