Trichoderma atroviride strain for producing myrosase and application thereof

A technology of Trichoderma dark green, Trichoderma dark green, applied in the application, fungicides, fungi and other directions, can solve the problems of low degradation, unstable fumigation effect, slow speed, etc., to achieve fast growth, strong soil and The effect of rhizosphere colonization ability and operability

Active Publication Date: 2012-07-25
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to: when using Brassica plants as soil biological fumigation materials, the GSLs therein degrade and release the biocidal gas ITCs at a slow speed, the degree of degradation is low, and the fumigation effect is unstable, and a kind of energy-metabolizing Trichederma atroviride, which produces myrosinase to promote the degradation of GSLs and release biocidal gas ITCs, has antagonism to plant pathogenic bacteria and promotes plant growth, fast growth, easy to cultivate, and strong soil colonization ability, is applied to soil Biological fumigation to improve its control effect on soil-borne pests and diseases

Method used

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  • Trichoderma atroviride strain for producing myrosase and application thereof
  • Trichoderma atroviride strain for producing myrosase and application thereof
  • Trichoderma atroviride strain for producing myrosase and application thereof

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Effect test

Embodiment 1

[0022]Jiangsu Academy of Agricultural Sciences screened a Trichoderma strain from the soil. The strain was preserved on December 23, 2011 in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures. The address of the preservation unit is: 1 Beichen West Road, Chaoyang District, Beijing No. 3 Courtyard, Institute of Microbiology, Chinese Academy of Sciences, postal code 100101. The deposit number is CGMCC No.5609,

[0023] The characteristics of the strain are: cultured on a PDA plate at 30°C for 4 days to cover a plate with a diameter of 90 mm, the surface of the mycelium is loose and flat, and initially white; on the third day of cultivation, spores can be clearly seen, initially light green, Afterwards, it will gradually increase and become dark green. The spore-forming clusters are arranged in concentric ring patterns, and the back of the culture medium is brownish-yellow. It was identified as Trichederma atroviride by means of morph...

Embodiment 2

[0029] Preparation of culture medium

[0030] Preparation of test tube slant medium PDA: Weigh 200 grams of potatoes, peel them, cut into pieces and boil for half an hour, then filter with two layers of gauze, add 20 grams of glucose and 20 grams of agar, add water to 1000 milliliters after melting, pack The amount is 1 / 5-1 / 6 of the height of the test tube, plug the test tube mouth with a test tube stopper, autoclave at 121°C for 25 minutes, take it out while it is hot, and put it on a slope until it is cooled for later use. The height of the slope does not exceed 1 / 3 of the height of the test tube.

[0031] Preparation of liquid seed medium PDB: Weigh 200 grams of potatoes, peel them, cut into pieces and boil for half an hour, then filter with two layers of gauze, add 20 grams of glucose, make up water to 1000 milliliters, and then divide into liquid volumes It is an 80-100ml / 250ml triangular flask, plug the mouth of the bottle with a cotton plug, autoclave at 121°C for 25 mi...

Embodiment 3

[0034] The preparation of the dark green Trichoderma (Trichederma atroviride) solid microbial agent that produces myrosinase (the various culture medium that this embodiment relates to even embodiment 2 provides)

[0035] a) Activation of strains

[0036] The method for activating the bacterial strains preserved on the inclined plane of the test tube: the bacterial classification preserved on the inclined plane of the test tube is selected to inoculate a Trichoderma mycelium block of CGMCC No.5609 with a preservation number of about 5 mm in diameter and inoculated into the culture medium PDA on the inclined plane of the test tube for activation. Incubate in an environment of 28±2°C for 3-5 days before use.

[0037] The method of activating the strains preserved by freeze-drying in ampoule tubes: scrub the ampoule with 70% alcohol cotton ball in the ultra-clean workbench, then make a groove in the ampoule with a grinding wheel, wrap the ampoule with sterile gauze pad or sterile...

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Abstract

The invention relates to a trichoderma atroviride strain for producing myrosase and application thereof. The file number of the dark-green trichoderma atroviride strain for producing myrosase is CGMCC (China general microbiological culture collection) No.5609. During use, trichoderma atroviride is applied to soil containing brassica plant tissue as fumigated materials in a solid bactericide manner, pests in the soil are killed by biological fumigation, and pest diseases spread by the crop soil are prevented.

Description

technical field [0001] The invention relates to a Trichoderma strain and its application. Specifically, it is a Penicillium atroviride (Trichederma atroviride) that can metabolize and produce myrosinase and has a strong antagonistic effect on common plant pathogenic fungi, and its application to promote the degradation of glucosinolates when using Brassica plants as soil fumigation materials. It belongs to the specific application of using microorganisms to biodegrade agricultural waste or agricultural by-product leftovers to improve their bioavailability or to realize high added value of waste. Background technique [0002] Soil-borne diseases are one of the important diseases that endanger vegetable production in facilities, and are mainly transmitted through soil, plant residues or pathogen residues in soil. Soil disinfection treatment is an important measure to control soil-borne diseases. Among them, soil fumigant represented by methyl bromide has been widely used in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 马艳常志州殷蒙徐跃定张建英
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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