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Bioluminescent nanoparticle with 800-nanometer strong near infrared up-conversion emission characteristic and application thereof

A technology with infrared up-conversion and emission characteristics, applied in the field of biofluorescent marker preparation and application, can solve the problems of limited application and long-term observation, and achieve the effect of high penetration ability

Inactive Publication Date: 2012-08-01
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although organic fluorophores and quantum dots are also capable of near-infrared emission, they have significant disadvantages as fluorescent probes compared to rare-earth-doped materials.
For example, near-infrared organic fluorophores are prone to photobleaching. In addition, their wide emission bands and small Stokes shifts limit the application of this material in the detection process and long-term observation.
At present, quantum dots, which are widely used, are also limited in their application in deep tissues due to their own toxicity and the fact that the excitation light source is in the visible region.

Method used

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  • Bioluminescent nanoparticle with 800-nanometer strong near infrared up-conversion emission characteristic and application thereof
  • Bioluminescent nanoparticle with 800-nanometer strong near infrared up-conversion emission characteristic and application thereof
  • Bioluminescent nanoparticle with 800-nanometer strong near infrared up-conversion emission characteristic and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Mix 40ml of oleic acid, 20ml of alcohol, and 16ml of water evenly, add 2.4g of NaOH under stirring to form a uniform milky white colloid, then add 1mmol of mixed rare earth compound aqueous solution (the mole percentage of rare earth ions is Yb(NO 3 ) 3 :20%; Tm(NO 3 ) 3 :0.5%; Y(NO 3 ) 3 :79.5% and Yb(NO 3 ) 3 :20%; Tm(NO 3 ) 3 :0.5%; Ho(NO 3 ) 3 :2%; Y(NO 3 ) 3 : 77.5%). Then, under rapid stirring, 8mmol NaF aqueous solution was added dropwise to form a uniform emulsion, and then transferred to the reaction kettle at 180°C for 19 hours. After the reaction is over, cool to room temperature naturally, take out the reaction solution and centrifuge it, pour it out, wash it with alcohol and deionized water and centrifuge it 3 times, and then dry it in a vacuum drying oven at 80°C to obtain NaYF 4 : Yb / Ho / Tm nano-single crystal, hexagonal plate with a side length of 130nm, its structure and morphology are as follows figure 1 As shown in the XRD and SEM photos. Pumped with a 9...

Embodiment 2

[0034] Add 1mmol of rare earth chloride salt LnCl 3 ·6H 2 O (Ln = 70% Lu, 18% Yb, 10% Ho, 2% Tm), 15 mL ODE (octadecene), 6 mL OA are placed in a 100 mL three-necked flask, and the system is elevated under the protection of argon And react for 30 min at 160℃, then cool the temperature of the system to room temperature, add 4mmol (0.148g) ammonium fluoride and 2.5mmol (0.1g) sodium hydroxide dissolved in 10mL of anhydrous methanol slowly dropwise Put it into a three-necked flask and keep stirring at room temperature for 60 minutes, then raise the temperature of the system to 50°C to remove the methanol solution in the reaction mixture, continue to rapidly increase the temperature to 300°C under the condition of passing protective gas, and keep it for 60 minutes, then stop heating. After the reaction system is naturally cooled to room temperature, an excess amount of absolute ethanol is used to precipitate the product, and a mixed solution of absolute ethanol, cyclohexane, and de...

Embodiment 3

[0036] At 40℃, the YbCl 3 : 4%; TmCl 3 :0.5%; YCl 3 : 95.5% and YbCl 3 : 4%; TmCl 3 : 0.5%; HoCl 3 :2%; YCl 3 : 93.5% were mixed uniformly, and stirred into a uniform transparent chloride solution. Then, urea was added to adjust the pH value, and precipitation occurred in the solution, and the temperature was raised to 80°C to complete the precipitation. Separate the precipitate with a centrifuge and rinse with deionized water repeatedly. The washed precipitate is dried and placed in a muffle furnace, slowly heated to 500°C, kept at a constant temperature for 3 hours, and cooled to room temperature. Then the cooled product is annealed at 800°C to obtain the required rare earth-doped single crystal sample Y 2 O 3 :Yb / Ho / Tm and Y 2 O3:Yb / Tm, the size of single crystal sample is about 200nm. Pumped with a 980nm laser, in Y doped with holmium ions 2 O 3 In the sample, the luminescence intensity near 800nm ​​of thulium ion is significantly improved compared with the luminescence in...

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Abstract

The invention belongs to the technical fields of preparation and application of a bioluminescent marker, and particularly relates to a strong near infrared up-conversion luminous bioluminescent nanoparticle of a Yb and Ho ion di-sensitized Tm ion and an application thereof. Through energy transmission among Yb / Ho / Tm in the up-conversion luminous material, higher-efficiency 800-nanometer near infrared up-conversion light transmission can be obtained under the induction of near infrared light of 900-1,064 nanometers. The nanoparticle has the advantages that: excited light and emitted light at positioned at a range of 750-1,000 nanometers of an optical window of a biological tissue, and enhanced near infrared up-conversion light can be emitted at a position of 800 nanometers. The near infrared light and visible light in the range have higher penetrating power in organisms, and the functions of fluorescence detection, tracing and the like of higher-layer biological tissues in organisms can be realized. The near infrared luminous strength of the obtained material close to the position of 800 nanometers is enhanced greatly, and the material is easy to detect, and has a simple preparation process.

Description

Technical field [0001] The invention belongs to the technical field of preparation and application of biological fluorescent markers, and specifically relates to a strong near-infrared up-conversion luminescent biological fluorescent nanoparticle of Yb and Ho ion double-sensitized Tm ion and its application. Background technique [0002] Fluorescence detection technology has the advantages of high sensitivity, good selectivity, multiple characteristic parameters, and wide dynamic range. It has been widely used in many fields such as life sciences and medicine. There are a large number of scientific research and related achievements transformation reports every year. Fluorescent markers can be used to display and track the conformational changes and dynamic processes of biological tissues, cells, and biomolecules in cells. Tracking the movement of biomolecules in living cells can not only accurately find the location of the mutant cells, but also understand the process of virus in...

Claims

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Application Information

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IPC IPC(8): C09K11/85C09K11/78C09K11/61C09K11/02C12Q1/68
Inventor 秦伟平王丽丽赵丹吴长锋秦冠仕郑克志揣晓红何春凤狄卫华
Owner JILIN UNIV
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