Preparation method and application for multifunctional nanometer diagnosis and treatment agent integrating light emitting/CT/MR multi-mode imaging and tumor hyperthermia

A multi-modal imaging and multi-functional technology, which can be used in medical preparations containing active ingredients, medical preparations without active ingredients, anti-tumor drugs, etc. It can solve problems such as ultraviolet excitation limiting biological applications, and achieve good CT imaging performance. , good dispersibility, the effect of simple and easy synthesis process

Inactive Publication Date: 2014-01-01
SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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Problems solved by technology

Quantum dots have been previously used as such three-mode probes in the literature, but their drawbacks such as toxicity and UV excitation severely limit their biological applications in vivo.

Method used

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  • Preparation method and application for multifunctional nanometer diagnosis and treatment agent integrating light emitting/CT/MR multi-mode imaging and tumor hyperthermia
  • Preparation method and application for multifunctional nanometer diagnosis and treatment agent integrating light emitting/CT/MR multi-mode imaging and tumor hyperthermia
  • Preparation method and application for multifunctional nanometer diagnosis and treatment agent integrating light emitting/CT/MR multi-mode imaging and tumor hyperthermia

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preparation example Construction

[0051] Another aspect of the present invention provides a preparation method of the above-mentioned multifunctional nano-diagnostic agent. Firstly, the hydrophobic NaYbF was prepared by high-temperature pyrolysis method. 4 :Er 3+ / Gd 3+ , and then through the inverse microemulsion method, a layer of amino-functionalized silica (positively charged on the surface) is coated on its outer surface, and then the negatively charged copper sulfide nanoparticles prepared in advance are adsorbed on the surface by means of electrostatic adsorption. surface, and finally graft polyethylene glycol (PEG) on the outermost surface. Specifically, as an example, the following steps may be included.

[0052] Preparation of NaYbF by High Temperature Pyrolysis 4 :Er 3+ / Gd 3+ The process of hydrophobic nanoparticles includes the following specific steps: mixing and stirring the rare earth ion precursor with oleic acid and octadecene, heating to remove water, raising the temperature to 140-180...

Embodiment 1

[0064] 1. NaYbF 4 :2%Er 3+ / 20%Gd 3+ Preparation of hydrophobic nanoparticles (2mmol group):

[0065] 1. Preparation of rare earth chlorides: Weigh 1.56mmol (604.4mg) YbCl respectively 3 ·6H 2 O, 0.04mmol (15.34mg) ErCl 3 ·6H 2 O and 0.4 mmol (148.7 mg) GdCl 3 ·6H 2 O powder, dissolved in 3mL deionized water for later use;

[0066] 2. Add 15mL of oleic acid and 30mL of octadecene into the three-neck flask, then add the pre-configured chloride aqueous solution, and stir at room temperature for 1h. Then start to pass argon gas for 15 minutes to remove the air in the bottle, and then the system starts a slow water removal process. After the water removal is completed, the system rises to about 160 degrees and keeps for 1 hour. A pale yellow clear liquid is obtained. Then stop heating and let the system cool down to room temperature naturally;

[0067] 3. Slowly add 15mL methanol solution (containing 200mg NaOH, 296mg NH 4 F), sealed and stirred at room temperature fo...

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Abstract

The invention relates to a preparation method and application for a multifunctional nanometer diagnosis and treatment agent integrating light emitting/CT/MR multi-mode imaging and tumor hyperthermia. The multifunctional nanometer diagnosis and treatment agent comprises up-conversion luminescence nano-particles, a silicon dioxide layer, near-infrared photothermal conversion agent particles and sulfhydryl functionalization polyethylene glycol, wherein the up-conversion luminescence nano-particles have the performance of near-infrared light emitting/CT/MR three-mode imaging; the surfaces of the up-conversion luminescence nano-particles are wrapped with the silicon dioxide layer; the near-infrared photothermal conversion agent particles are in electrostatic adsorption on the surface of the silicon dioxide layer; the sulfhydryl functionalization polyethylene glycol is grafted onto the surfaces of the near-infrared photothermal conversion agent particles. The multifunctional nanometer diagnosis and treatment agent provided by the invention can integrate light emitting/CT/MR multi-mode imaging and the tumor hyperthermia into a whole, and through the stimulation of a single wave band, the light emitting imaging and tumor hyperthermia can be achieved simultaneously.

Description

technical field [0001] The present invention relates to a multi-functional nano-diagnosis and treatment agent integrating luminescence & CT & MR multi-mode imaging and tumor hyperthermia and its preparation method and application. Specifically, it is to design and prepare a novel multi-functional nano-therapy agent. The agent has good biocompatibility, will not cause toxic damage to living tissues and organs, has superior CT&MR&luminescent three-mode imaging performance, and can convert the light energy of near-infrared light into heat energy to kill cancer cells. It belongs to nanobiomedicine The scope of diagnosis and treatment. Background technique [0002] CT imaging has been widely used in the field of clinical diagnosis, and its main advantages are that it can realize three-dimensional tomographic imaging, and there is basically no limit to the penetration depth of X-rays. The currently used CT contrast agent is mainly small molecule iodine agent, which is cheap, but ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18A61K41/00A61K47/48A61K49/04A61P35/00
Inventor 肖庆风步文博施剑林
Owner SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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