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Method for constructing finger-print chromatogram for ginsenosides-containing crude drugs and preparations

A technology of ginsenosides and fingerprints, which is applied to the analysis of materials, material separation, and measuring devices, etc. It can solve problems such as inability to control quality, difficult to achieve baseline separation, and incomplete analysis of characteristic components, so as to achieve accurate quality control and ensure stability. Effects of Sex and Consistency

Inactive Publication Date: 2012-08-01
JILIN ACAD OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the quality control of medicinal materials containing ginsenosides and their extracts is mainly studied by high performance liquid chromatography (HPLC), but because their main components are triterpene saponins, such as: Saponin Rg 1 and Re, ginsenoside Rb 2 and Rb 3 etc. It is difficult to achieve baseline separation on HPLC. To achieve better separation, it often takes a long analysis time, usually 1 to 2 hours; in addition, some triterpenes contained in American ginseng stems and leaves Saponins, such as pseudoginsenoside F 11 , does not have ultraviolet absorption, and the content is relatively high, and has good pharmacological activity. Commonly used ultraviolet (UV) and diode array detectors (DAD) cannot detect such substances. Therefore, the analysis of characteristic components is not comprehensive and cannot Lack of overall quality control of American ginseng, American ginseng stems and leaves and other medicinal materials containing ginsenosides and their extract preparations

Method used

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  • Method for constructing finger-print chromatogram for ginsenosides-containing crude drugs and preparations
  • Method for constructing finger-print chromatogram for ginsenosides-containing crude drugs and preparations
  • Method for constructing finger-print chromatogram for ginsenosides-containing crude drugs and preparations

Examples

Experimental program
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Effect test

Embodiment 1

[0045] American ginseng stems and leaves

[0046]1) Preparation of the test solution: Weigh 0.2 g of American ginseng stems and leaves, put it in a Soxhlet extractor, add 100 ml of chloroform, heat and reflux for 1 hour, discard the chloroform solution, evaporate the dregs of the chloroform, add 50 ml of methanol, Heat to reflux for 3 hours, evaporate the extract to dryness at low temperature, add 10 ml of water to dissolve, add petroleum ether (30~60°C) to extract twice, 10 ml each time, discard the ether solution, and pass the water through a D101 macroporous adsorption resin column (1.5 cm inner diameter, 15 cm column length), elute with 70 ml of water, discard the water, then elute with 50 ml of 20% ethanol, discard the 20% ethanol eluate, and then use 80% ethanol 80 ml for elution, collect 70 ml of the eluate, evaporate to dryness, dissolve the residue in methanol, put it in a 10 ml volumetric flask, and constant volume, as the test solution, pass through a 0.22 μm memb...

Embodiment 2

[0083] American ginseng root

[0084] 1) Preparation of the test solution: Weigh 1 g of American ginseng root, put it in a Soxhlet extractor, add 100 ml of methanol, heat and reflux for 3 hours, evaporate the extract to dryness at low temperature, add 50 ml of water to dissolve, add petroleum ether (30 ~60°C) twice, 50 ml each time, discard the ether solution, pass the water solution through a D101 macroporous adsorption resin column (1.5 cm inner diameter, 15 cm column length), elute with 100 ml water, discard water solution, then elute with 100 ml of 20% ethanol, discard the 20% ethanol eluate, continue to elute with 150 ml of 80% ethanol, collect 100 ml of eluate, evaporate to dryness, add methanol to dissolve the residue, and place in 10 ml In a volumetric flask, constant volume was used as the test solution, and passed through a 0.22 μm filter membrane before analysis.

[0085] 2) Preparation of reference solution: accurately weigh ginsenoside Rb 1 , dissolved in meth...

Embodiment 3

[0111] ginseng root

[0112] 1) Preparation of the test solution: Weigh 2 g of ginseng root to be tested, add 40 ml of chloroform, heat and reflux for 1 hour, discard the chloroform solution, evaporate the dregs of the chloroform, extract with 80 ml of water or methanol or n-butanol , evaporate the solvent, add 30 ml of water to dissolve, add 30 ml of petroleum ether (30~60°C) to extract 1-4 times, 30 ml each time, discard the ether solution, pass the water through a macroporous adsorption resin column, and wash with 50 ml of water ml, 60 ml of 20% ethanol and 70 ml of 75% ethanol were eluted sequentially, the 75% ethanol eluate was collected, evaporated to dryness, the residue was dissolved in methanol, set to volume in a 250 ml volumetric flask, and filtered through a filter membrane to obtain the test product product solution.

[0113] 2) Preparation of reference solution: accurately weigh ginsenoside Rb 1 , dissolved in methanol to make every 1 ml containing ginsenosid...

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Abstract

The invention provides a method for constructing a finger-print chromatogram for ginsenosides-containing crude drugs and preparations and relates to a construction method for constructing the ultra high performance liquid chromatography for preparations of American ginseng and American ginseng stem leaf drugs as well as American ginseng and American ginseng stem leaf extractives and a standard finger-print chromatogram thereof. The finger-print chromatogram obtained through the invention can be used for comprehensively controlling the quality of the drugs, ginsenosides extractives extracted from the drugs, and extractive preparations; and the preparations include solid preparations and liquid preparations, such as injections, granules, tablets, capsules, drinks, mixtures and oral liquids.

Description

[0001] technical field [0002] The invention belongs to the technical field of quality control methods for preparations of Chinese medicinal materials and their extracts, and further provides a fingerprint of preparations made from medicinal materials containing ginsenosides and their extracts, involving American ginseng, American ginseng stems and leaves, ginseng, ginseng The construction method and standard fingerprint of the ultra-high performance liquid chromatography fingerprint of the stem and leaf, notoginseng, notoginseng stem and leaf medicinal materials and their extracts. Background technique [0003] The medicinal materials and preparations containing ginsenoside components in the present invention include: American ginseng, American ginseng stems and leaves, panax ginseng, panax ginseng stems and leaves, Sanqi, Sanqi stems and leaves medicinal materials and one or more mixtures prepared from their extracts. [0004] At present, the quality control of medicinal ...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 徐雅娟刘悦解生旭徐东铭
Owner JILIN ACAD OF TRADITIONAL CHINESE MEDICINE
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