Gold nanoparticle with core-shell structure and preparation method thereof
A gold nanoparticle, core-shell structure technology, applied in the field of nanomaterials, can solve the problems of poor stability and solubility, no stability factor, wide size distribution of gold nanoparticles, etc., and achieve good stability and good temperature responsiveness. Effect
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[0026] The invention provides a method for preparing gold nanoparticles with a core-shell structure, comprising the following steps: A) poly(ethylene glycol-block-N-isopropylacrylamide) copolymer with a dithioester group at the end (PEG-b-PNIPAM) undergoes an aminolysis reaction with an aminolysis reagent to obtain a monomercapto-terminated poly(ethylene glycol-block-N-isopropylacrylamide) copolymer (PEG-b-PNIPAM-SH); B) Carrying out ligand exchange reaction between the poly(ethylene glycol-block-N-isopropylacrylamide) copolymer terminated by monomercapto and gold nanoparticles with sodium citrate as a ligand to obtain a core-shell structure Gold Nanoparticles.
[0027] Wherein, the PEG-b-PNIPAM is preferably prepared according to the reversible addition-fragmentation chain transfer (RAFT) method:
[0028] S1) Add monomethyl-terminated polyethylene glycol (mPEG-OH) and dry toluene solution into a container, under nitrogen protection, heat until mPEG-OH is completely dissolved...
Embodiment 1
[0041] 1.1 Synthesis of dithiobenzoic acid (DTBA)
[0042] Add 22.5g of 30% sodium methoxide solution, 4g of dry sulfur powder in a 250mL three-necked round bottom flask equipped with a stirring bar, a constant pressure dropping funnel and a spherical condenser, and slowly add 7.95 g benzyl, react at room temperature for 30 minutes after completion, then raise the temperature to 70°C and react overnight. After cooling in an ice-water bath, the insoluble matter was filtered off, and the solvent was removed under reduced pressure. The residue was re-dissolved in water, acidified by adding ether and 1.0mol / L hydrochloric acid to convert it into DTBA. Repeated extraction three times with iced sodium hydroxide solution and hydrochloric acid / ether solution, and finally obtained 6.6 g of pure red oily liquid as DTBA, with a yield of about 60%.
[0043] 1.2 Addition reaction of mPEG-OH with MAh
[0044] Add 8.0g mPEG-OH and 30mL dry toluene solution into a 100mL round bottom flask,...
Embodiment 2
[0076] Prepare each intermediate and final product in the same manner as in Example 1, only block copolymer PEG-b-PNIPAM is synthesized as follows:
[0077] Add 1.0 mg of azobisisobutyronitrile, 0.80 g of NIPAM, 0.20 g of the mPEG-CAT obtained in 1.4 and 5.5 mL of dry acetonitrile solution into a polymerization tube equipped with a stirring bar, and freeze-thaw the reaction mixture three times. Gas process, seal the tube under vacuum, and react at 60°C for 24h. The obtained product was precipitated three times with ether, and dried in vacuum at 50° C. for two days to obtain a block copolymer PEG-b-PNIPAM with a monomer conversion rate of about 30%.
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