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Extraction method of glucan in fig leaf

A fig leaf and extraction method technology, applied in the field of glucan extraction, can solve the problems of fig leaf waste and failure to be developed and utilized, and achieve the effects of mild extraction conditions, effective value-added utilization, and low production cost

Inactive Publication Date: 2012-08-22
WEIHAI XINYI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The amount of fig planting in my country is increasing year by year, and a fig planting base with considerable advantages has been formed. Its fig leaf resources are relatively rich, and the scientific and timely picking of fig leaves has little effect on the original plants. However, a large number of fig leaves are currently discarded and cannot be developed and utilized. , there is no report of extracting dextran from fig leaves

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Harvest the fresh fig leaves with stalks in August, wash them with flowing water, send them to a tissue grinder for grinding, and then grind the ground fig leaves with a colloid mill, add 2 times of water and grind them into 100 The target slurry is then transferred to an ultrasonic reaction kettle, add 7 times of water, heat to 55°C for ultrasonic treatment, ultrasonic power is 800W, ultrasonic treatment is performed for 3 hours, and then the supernatant is collected by centrifugation and concentrated under reduced pressure to obtain a concentrated solution. Then add ethanol until the volume concentration of ethanol is 80% for alcohol precipitation, take the precipitate and add 9 times the mass of water to redissolve, perform ultrafiltration with a membrane with a molecular cut-off of 10,000, and concentrate the retentate under reduced pressure to 1 / 3 of its original volume Afterwards, freeze-dry to a moisture content of 3% to obtain the β-glucan of the pres...

Embodiment 2

[0020] Example 2: Harvest the fresh fig leaves with stalks in July, wash them with flowing water, send them to a tissue grinder for grinding, then grind the ground fig leaves with a colloid mill, add 2 times of water and grind them into 100 The target slurry is then transferred to an ultrasonic reactor, add 6 times of water, heat to 52°C for ultrasonic treatment at a constant temperature, the ultrasonic power is 500W, and the ultrasonic is applied for 4 hours, then the supernatant is collected by centrifugation and concentrated under reduced pressure to obtain a concentrated solution. Then add ethanol until the volume concentration of ethanol is 80% to carry out alcohol precipitation. After alcohol precipitation, the slurry is vacuum-dried to a moisture content of 4% at a temperature of 70°C and a pressure of 0.08Mpa to obtain the dextran of the present invention.

Embodiment 3

[0021] Example 3: Harvest the fresh fig leaves with stalks in September, wash them with running water and send them to a tissue grinder for grinding, then grind the ground fig leaves with a colloid mill, add water 3 times and grind them into 100 The target slurry is then transferred to an ultrasonic reaction kettle, added 5 times of water, heated to a constant temperature of 52°C for ultrasonic treatment, and the ultrasonic power is 800W, and the ultrasonic is applied for 3 hours, and then the supernatant is collected by centrifugation and concentrated under reduced pressure to obtain a concentrated solution. Then add ethanol until the volume concentration of ethanol is 80% for alcohol precipitation, take the precipitate and add 7 times the mass of water to redissolve, perform ultrafiltration with a membrane with a molecular cut-off of 10,000, and concentrate the retentate under reduced pressure to 1 / 3 of its original volume Afterwards, freeze-dry to a moisture content of 3% ...

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PUM

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Abstract

The invention relates to an extraction method of glucan in a fig leaf. The extraction method is characterized by comprising the following steps of: cleaning and rubbing a collected fresh fig leaf, and then, adding water to mill the fig leaf into thick liquid by using a colloid mill, afterwards, feeding the thick liquid into an ultrasonic reaction kettle, adding 5-8 times of water, carrying out ultrasonic processing for the ultrasonic acting time of 3-5 hours at a constant temperature of 45-55 DEG C, and finally, preparing the glucan through process steps such as centrifugation, concentration under reduced pressure, alcohol precipitation and vacuum drying, and the extraction method can further adopt the following steps of: adding 7-9 times of water in mass to redissolve after the alcohol precipitation, carrying out ultrafiltration by a membrane with a molecular interception amount of 10000, subjecting an intercepted solution to the concentration under reduced pressure again, and then, carrying out freeze drying on the intercepted solution to obtain beta-glucan with better activity. The extraction method is simple in process; the production cost is low; extraction conditions are mild; the pollution does not exist; the purity is high; the effective value-added utilization of the fig leaf is realized; the abundant abandoned fig leaves are changed from the waste into the valuable things; the market development prospect of the extraction method is wide; and the meaning is significant.

Description

technical field [0001] The invention belongs to the technical field of food and medicine processing, and in particular relates to a method for extracting glucan from fig leaves. Background technique [0002] In the 21st century, scientists have discovered that sugar plays a more important role in living organisms than protein, especially in the field of biomedicine. Polysaccharides can serve as very good drug carriers, and are vividly called "biological missiles" by scientists. In polysaccharide products, dextran occupies an important position, and the core substance of polysaccharides in natural medicine is dextran. [0003] Dextran is usually a variety of naturally occurring blood polysaccharides or polydextrose, including polymers such as cellulose, amylose, glycogen, laminarin, starch, etc., dextran contains branched and unbranched chains, composed of 1 Glucose connected by →3, 1→4, and 1→6 glycoside chains. The glycoside chains can be α and β types. It is a macromolecu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02
Inventor 方华王华丽王生虎王金玲李增绪周少华
Owner WEIHAI XINYI BIOLOGICAL TECH
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