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Preparation method of microorganism immobilization particles for removing residual pesticide in soil

A technology of immobilization of microorganisms and residual pesticides, applied in the field of agricultural biology, can solve the problems of less research on the degradation of organic phosphorus pesticides in pesticide production wastewater, and achieve the effects of good ventilation, low cost and low price

Inactive Publication Date: 2012-09-05
中华人民共和国沈阳出入境检验检疫局 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0020] In recent decades, applied research on immobilization technology has been carried out at home and abroad, especially in the treatment of refractory organic wastewater, high-concentration organic wastewater, ammonia nitrogen wastewater, wastewater containing heavy metals, and printing and dyeing wastewater. There are relatively few studies on the degradation of organophosphorus pesticides and pesticide production wastewater. The application of immobilized microbial technology to the repair of organic-contaminated soil is still in its infancy at home and abroad, and there are few reports on the treatment of organophosphorus pesticide-contaminated soil.

Method used

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  • Preparation method of microorganism immobilization particles for removing residual pesticide in soil
  • Preparation method of microorganism immobilization particles for removing residual pesticide in soil
  • Preparation method of microorganism immobilization particles for removing residual pesticide in soil

Examples

Experimental program
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Effect test

Embodiment 1

[0037] A preparation method for removing microbial particles of pesticide residues in soil, according to the following steps:

[0038] (1) Crush the zeolite into particles with a particle size of 60 mesh, dry the corncobs at constant temperature to a constant weight, and then crush them into particles with a particle size of 40 mesh. The core is mixed at a ratio of 1:5 and used as a carrier, dried for later use;

[0039] (2) Fully mix the volume of the Clostridium strain with the weight of the carrier according to the ml:g ratio of 1:0.1, and use glutaraldehyde with a concentration of 0.5% as the cross-linking agent to stir evenly. The volume ratio of the cross-linking agent is 3:7, stir evenly, place at 4°C for 2 hours for cross-linking, prepare immobilized particles with a particle size of 2.5 mm after cross-linking, wash and dry with distilled water, that is, the described Microbial particles.

[0040] The composite immobilized microorganisms carried out the soil remediat...

Embodiment 2

[0042] A preparation method for removing microbial particles of pesticide residues in soil, according to the following steps:

[0043] (1) Crush zeolite into particles with a particle size of 60 mesh, dry corncobs at constant temperature to constant weight, and then crush them into particles with a particle size of 80 mesh. The core is 1:1 mixed and used as a carrier, dried for later use;

[0044] (2) Fully mix the volume of the Clostridium strain with the weight of the carrier at a ratio of 1:0.5 in ml:g, and stir evenly with 0.5% glutaraldehyde as the cross-linking agent. The volume ratio of the cross-linking agent is 1:5, stir evenly, place at 4°C for 6 hours for cross-linking, prepare immobilized particles with a particle size of 5.0mm after cross-linking, wash and dry with distilled water, and then make the described Microbial particles.

[0045] The composite immobilized microorganisms carried out soil remediation experiments on methyl parathion. The microbial particle...

Embodiment 1 Embodiment 2 and comparative example 1

[0049] Example 1, Implementation 2 and Comparative Example 1, the volumes of the bacterial liquid of Clostridium strains were large-diameter corncobs (40 mesh, S2), small-particle-diameter corncobs (80 mesh, S3) and sodium alginate (40 mesh , S1) The effect of different immobilized carriers on the degradation of methyl parathion by Clostridium strain A3 as shown in figure 1 shown. In soil remediation, the degradation effect of bacteria S1 immobilized with sodium alginate was lower than that of bacteria S2 immobilized with large-sized corncobs, while the degradation effect of bacteria S2 immobilized with large-sized corncobs was less than that of bacteria immobilized with small-sized corncobs. Degradation effect of bacteria S3. And as far as the carrier is concerned, the corn cob is non-toxic and harmless to the bacteria and soil, and can be used as a C source in the soil environment, while the sodium alginate carrier is a toxic substance to the soil.

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Abstract

The invention discloses a preparation method of microorganism immobilization particles for removing residual pesticide in soil, belonging to the field of agricultural biotechnology. The method comprises the following steps of: crushing the environment-friendly mineral particles and the plant waste dried at constant temperature to constant weight into particles, and mixing the particles proportionally to obtain a carrier; sufficiently mixing the liquid of the microorganism strain and the carrier according to the volume-to-weight ratio ml:g of (1:0.1)-(1:0.5); uniformly stirring by taking glutaraldehyde as a cross-linking agent and standing at 4 DEGE C for 2-6 hours, and performing cross-linking to obtain immobilization particles; and washing the immobilization particles with distilled water and drying to obtain the microorganism particles. According to the invention, the carrier material is low in cost, widely available and non-toxic to organisms, has good air permeability and does not cause secondary pollution to the soil; and the carrier and the immobilization technology thereof can be applied to the immobilization of the microorganisms of multiple strains such as fusobacterium and the like, and the microorganism immobilization particles are directly spread into the soil without damaging the original soil structure.

Description

technical field [0001] The invention belongs to the field of agricultural biology, in particular to a method for preparing microorganism immobilized particles for removing residual pesticides in soil. Background technique [0002] Pesticides are an important category of chemicals, which play an extremely important role in preventing, killing, repelling or reducing crop pests and weeds and ensuring stable and high yields. However, a large number of facts show that the widespread use of pesticides has also become one of the important factors causing environmental pollution and human health. In 1970, the United States established the National Environmental Protection Agency (US EPA) and began to conduct comprehensive environmental monitoring of products including pesticides, which marked the gradual evolution of the bipolar relationship between agriculture and pesticides into a tripolar relationship between agriculture-pesticides-environment Not only to promote the development...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/02B09C1/10C12R1/145C12R1/07C12R1/01
Inventor 姜莉姜彬慧王琳琳付丽丽冯迪
Owner 中华人民共和国沈阳出入境检验检疫局
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