Pseudomonas capable of removing nitrogen and phosphorus synchronously at low temperature and application thereof
A technology for Pseudomonas and denitrification and phosphorus removal, which is applied in the field of Pseudomonas strains for low-temperature biological denitrification and phosphorus removal, can solve the problem that nitrate nitrogen and nitrite nitrogen cannot be effectively removed, and the total nitrogen in effluent is difficult to reach the standard and increase. problems such as operating costs, to achieve the effect of saving infrastructure investment and operating costs, simplifying technological processes, and having broad application prospects
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Embodiment 1
[0033] Example 1. Temperature characteristics of bacterial strain HA11
[0034] Investigate the growth performance of the strain under different temperature conditions of 10°C, 15°C, 20°C, 25°C, and 30°C. The specific implementation steps are as follows:
[0035] Inoculate strain HA11 in 150ml of BM medium (containing sodium acetate per liter: 2.73g, NH 4 Cl: 0.306g, KH 2 PO 4 : 0.1g, MgSO 4 ·7H 2 O: 0.1g, FeSO 4 ·7H 2 O: 0.001g, pH: 7.0-7.3, steam sterilized at 121°C for 20 minutes), and pre-cultivated on a shaker at 10°C, 15°C, 20°C, 25°C, 30°C, 160r / min. When the strain grows to the late logarithmic phase, take 10ml of the bacterial liquid and put it into 150ml of fresh BM medium, and carry out shaking culture at 10°C, 15°C, 20°C, 25°C, 30°C, and 160r / min. Take the reaction solution at regular intervals and measure the OD 600 , to characterize the growth of the strain.
[0036] The result is as figure 1 As shown, the strain grows best at 15°C, and the growth rate ...
Embodiment 2
[0037] Example 2. Removal of Phosphate and Nitrogen by Strain HA11 at 10°C
[0038] Sodium acetate was used as the organic carbon source, ammonia nitrogen was used as the nitrogen source, and phosphate was used as the phosphorus source, and the nitrogen and phosphorus removal ability of the bacterial strain HA11 was determined. The specific implementation steps are as follows:
[0039] The strain HA11 was inoculated in 150ml of BM medium, and pre-cultured on a shaker at 10°C and 160r / min. When the strain grows to the late logarithmic growth phase, take 10ml of the bacterial liquid and put it into fresh 150ml BM medium, and carry out shaking culture at 10°C and 160r / min. The reaction solution was taken at regular intervals, and after being centrifuged at 8000 rpm for 10 minutes, the supernatant was taken to measure the concentration of various nitrogen-containing compounds and phosphate.
[0040] The result is as figure 2It can be seen that the concentrations of nitrogen an...
Embodiment 3
[0041] Example 3. Removal of nitrogen by bacterial strain HA11 at different temperatures
[0042] The denitrification performance of the strain was investigated at different temperatures of 10°C, 15°C, 20°C, and 25°C. The specific implementation steps are as follows:
[0043] The strain HA11 was inoculated in 150 ml of BM medium, and pre-cultured on a shaker at 10°C, 15°C, 20°C, 25°C, 160r / min. When the strain grows to the late logarithmic phase, take 10ml of the bacterial liquid and put it into fresh 150ml BM medium, and carry out shaking culture at 10°C, 15°C, 20°C, 25°C and 160r / min respectively. The reaction solution was taken at regular intervals, and after being centrifuged at 8000 rpm for 10 minutes, the supernatant was taken to measure the concentration of various nitrogen-containing compounds.
[0044] The result is as image 3 As shown, the strain can effectively remove total nitrogen in the temperature range of 10°C-25°C. At 15°C, the average removal rate of tota...
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