Preparation method of dry powder and fungus powder containing Tricholoma lobayense Heim oxalate decarboxylase

A technology of oxalate decarboxylase and acid decarboxylase, which is applied in the field of preparation of dry powder and bacterial powder, can solve the problem that oxalate cannot be degraded, prevent or treat urinary hyperoxalate and calcium oxalate stones, and is easy to prepare, low cost effect

Active Publication Date: 2012-10-03
WUHAN KANGFUDE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] A simple and effective way to degrade oxalate in the gut has not yet been found

Method used

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preparation example Construction

[0030] The preparation method of the dry powder and bacterial powder containing jinfu mushroom oxalate decarboxylase provided by the embodiment of the present invention comprises the following steps:

[0031] A. Using edible fungi such as Tricholoma Lobayensc Heim or its genus as raw materials, a large number of bacteria are cultivated by liquid fermentation, and then the pH value of the fermentation broth is adjusted to between 2.0 and 5.0 to induce the production of oxalate decarboxylase , and then collect the fermentation broth and cells. The process of liquid fermentation is as follows: the composition of the liquid medium is: yeast powder, 7 g / L; soybean peptone, 6 g / L; KH 2 PO 4 , 2 g / L; MgSO 4 ·7·H 2 O, 0.5 g / L; CaCl 2 , 0.2 g / L; glucose, 10 g / L; sucrose, 20 g / L; after sterilizing the liquid medium at 121°C for 30 minutes, cultivate the mycelium of golden phlox at 25°C. 10% at a rotational speed of 150-200 rpm, or fermenter culture at a temperature of 25°C and a ro...

Embodiment 1

[0036] Embodiment 1, steps are as follows:

[0037] A. Using edible fungus such as Jinfu mushroom or its similar fungi as raw materials, a large number of bacteria are cultivated by liquid fermentation. The composition of the liquid medium is: yeast powder, 7 g / L; soybean peptone, 6 g / L; KH 2 PO 4 , 2 g / L; MgSO 4 ·7·H 2 O, 0.5 g / L; CaCl 2 , 0.2 g / L; glucose, 10 g / L; sucrose, 20 g / L; after sterilizing the liquid medium at 121°C for 30 minutes, cultivate the mycelium of golden phlox at 25°C. 10% at a rotational speed of 150-160 rpm, or fermenter culture at a temperature of 25°C and a rotational speed of 200-220 rpm; the inoculum size is 10-15%, and after 3-4 days of cultivation, the fermentation broth Adjust the pH to 4.5~5.0 to induce the production of oxalate decarboxylase, and add MnCl at a concentration of 0.001~1 mM to the culture medium 2 . After 5-7 days of induced production, the fermentation broth and cells were collected. Both the bacteria and the fermentation b...

Embodiment 2

[0040] Embodiment 2, steps are as follows:

[0041] A. Using edible fungus such as Jinfu mushroom or its similar fungi as raw materials, a large number of bacteria are cultivated by liquid fermentation. The composition of the liquid medium is: yeast powder, 7 g / L; soybean peptone, 6 g / L; KH 2 PO 4 , 2 g / L; MgSO 4 ·7·H 2 O, 0.5 g / L; CaCl 2 , 0.2 g / L; glucose, 10 g / L; sucrose, 20 g / L; after sterilizing the liquid medium at 121°C for 30 minutes, cultivate the mycelium of golden phlox at 25°C. 10% at a rotational speed of 165-175 rpm, or fermenter culture at a temperature of 25°C and a rotational speed of 230-250 rpm; the inoculum size is 16-20%, and after 5-6 days of cultivation, the fermentation broth Adjust the pH to 4.0~4.5, induce the production of oxalate decarboxylase, and add MnCl at a concentration of 2~30 mM to the culture medium 2 . After 8-10 days of induced production, the fermentation broth and cells were collected. Both the bacteria and the fermentation broth...

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Abstract

The invention discloses a preparation method of dry powder and fungus powder containing Tricholoma lobayense Heim oxalate decarboxylase and relates to the field of degradation of oxalate in stomachs and intestines of human bodies or animal bodies. The preparation method comprises the following steps of: using Tricholoma lobayense Heim or sibling fungi thereof as raw materials, culturing thalli by adopting a liquid fermentation method, regulating pH (potential of hydrogen) of fermentation solution to 2.0-5.0, inducing to produce oxalate decarboxylase, collection the fermentation solution and the thalli, crushing the thalli, conducting solid-liquid separation to respectively obtain liquid and solid containing the Tricholoma lobayense Heim oxalate decarboxylase, and respectively drying the obtained liquid and solid after separation to obtain the dry powder and the fungus powder containing the Tricholoma lobayense Heim oxalate decarboxylase. The dry powder and the fungus powder containing the Tricholoma lobayense Heim oxalate decarboxylase can be used for oxalic acid removal of food, beverage, feed and the like, can also be used as raw materials to prepare healthcare products or medicines, is suitable for oral taking, can effectively degrade oxalic acid and oxalate and can prevent or cure urine oxalic acid excess symptoms and calcium oxalate calculi.

Description

technical field [0001] The invention relates to the field of oxalate degradation in the gastrointestinal tract of a human body or an animal body, in particular to a method for preparing dry powder and bacterial powder containing jinfu mushroom oxalate decarboxylase. Background technique [0002] Stone disease afflicts tens of millions of people worldwide each year and causes economic losses in the tens of billions of dollars. Calcium oxalate stones are the most common stones, accounting for about 75% of all stones. Oxalate in the human body mainly comes from two ways: absorption from diet and physiological metabolism. Except for a very small number of patients with primary oxalic acid disease, the oxalate produced by the physiological metabolism of normal people is generally within a certain range, with little change, but the amount of oxalate absorbed from the diet varies from person to person. Patients with calcium oxalate stones absorb an average of 50% more oxalate fro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A23L1/28C12N9/88C12R1/645A61K36/06A61K9/14A61P13/04A23L33/00
Inventor 李青山阮荣刘海峰宋保平陈先桥
Owner WUHAN KANGFUDE BIOTECH CO LTD
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