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System for detecting breast cancer

A breast cancer and buffer technology, applied in the field of medicine, can solve the problems of quantification, inability to generate, increase the detection cost and detection difficulty, etc., and achieve the effect of simple operation and short detection time.

Active Publication Date: 2014-07-09
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are many clinical diagnostic methods for breast cancer, such as inspection, palpation, mammography, B-ultrasound examination, breast light-scattering imaging, mammary duct endoscopy, breast MRI examination, and pathological biopsy, etc., but these methods require Good operating skills and rich clinical experience are a relatively subjective judgment method, which cannot produce a quantitative result, which increases the cost and difficulty of detection

Method used

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  • System for detecting breast cancer
  • System for detecting breast cancer
  • System for detecting breast cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Feed 50 nude mice, 20 of which were used as blank controls, and 30 of them were inoculated with breast cancer cells (MCF-7) in the nude mice. After the tumors grew, tumor tissues were taken from the nude mice inoculated with MCF-7, and the blank control group was directly Take mammary gland and nearby muscle cells, make a single cell suspension, wash and suspend with sodium phosphate-sodium hydrogen phosphate buffer solution with a pH value of 6, and obtain 20 parts with a concentration of 10 4 ~10 9 cells / mL of normal cell suspension samples and 30 copies with a concentration of 10 4 ~10 9 cells / mL of tumor cell suspension samples.

[0038] Add the compound of the following formula to each sample so that the concentration of the compound in each sample is 0.5 μM

[0039]

[0040] Each sample was incubated in a 37°C incubator for 1 hour, followed by an additional 1 hour in a 4°C refrigerator.

[0041]After the incubation, the samples were centrifuged to remove th...

Embodiment 2

[0046] The same steps as in Example 1 were used to detect the tumor cells and normal cells of the above 50 nude mice, the difference being that the compounds used were:

[0047]

[0048] The concentration of the compound in the sample was 50 μM, and the incubation temperature and time were 15 minutes at 37° C. and 15 minutes at 4° C., and then the staining rate of each sample was obtained by using the fluorescence signal above 670 nm of the flow cytometer sample.

[0049] Test results: the staining rates of all breast cancer samples are above 90%, and the staining rates of all control samples are below 20%.

Embodiment 3

[0051] The same steps as in Example 1 were used to detect the tumor cells and normal cells of the above 50 nude mice, the difference being that the compounds used were:

[0052]

[0053] The compound concentration was 10 μM, the incubation temperature and time were 7.5 hours at 37°C, and 0.5 hours at 4°C, and the fluorescence signal above 670nm was detected by flow cytometry to obtain the staining rate of each sample.

[0054] Test results: the staining rates of all breast cancer samples are above 80%, and the staining rates of all control samples are below 25%.

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Abstract

The invention provides a system for detecting breast cancer. The system comprises reagent I, reagent II and a flow cytometer, wherein the reagent I is buffer liquid of which the pH value is 6-8; the reagent II is a compound comprising a structure described in the formula I. The sensitivity of a diagnostic system is over 100 times that of a traditional single-molecule probe, and the accuracy rate is about 97-99%; the system has the advantages of simple operation and short detection time; the result is quantized numerical values, no description or experience is needed, and the result is more objective and accurate.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a supramolecular probe that can be used for diagnosing breast cancer. Background technique [0002] Breast cancer is one of the most common malignant tumors in women, and it is the most common malignant tumor that seriously affects women's physical and mental health and even threatens their lives. The latest statistics show that the incidence of breast cancer ranks first among all types of cancer, and the mortality rate is second only to lung cancer. The death toll accounts for 14% of various cancers, and it is a very serious tumor disease. [0003] The etiology of breast cancer has not been fully clarified, but factors such as infertility, low number of births, late age at full-term birth of the first child, early age at menarche, history of benign breast disease, family history of breast cancer, oral contraceptives, and radiation exposure have been identified. Confirmed to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/14
CPCC09B23/0008C09B23/06G01N15/1459G01N2015/1006G01N2015/1488
Inventor 唐亚林姜薇杨千帆孙红霞尚倩盖伟向俊锋
Owner INST OF CHEM CHINESE ACAD OF SCI
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