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Apparatuses and methods for portable mass spectrometry

一种质谱分析、质量分析器的技术，应用在便携式光谱仪、质谱仪、动态光谱仪等方向，能够解决限制等问题

Active Publication Date: 2012-10-24
ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Prior to the present invention, the upper limit of analyte size for MS suitable for use with portable instruments was limited, and even more limited in non-high vacuum (e.g., -5 ability to analyze macromolecules under

Method used

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  • Apparatuses and methods for portable mass spectrometry
  • Apparatuses and methods for portable mass spectrometry
  • Apparatuses and methods for portable mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0123] Embodiment 1: the structure of portable mass spectrometer

[0124] A portable mass spectrometer was constructed using synthetic poly(methyl methacrylate) for the vacuum chamber. The instrumentation includes a KNF diaphragm pump and an Alcatel turbomolecular pump to provide the vacuum. All parts of the instrument are contained in a space 30 cm long, 28 cm high and 25 cm wide. The total mass of the instrument is about 16 kg. The mass analyzer is capable of measuring m / z ratios from about 500 to about two million. The instrument consists of dimensions r 0 =10mm and z 0 =7.07mm ion trap. exist figure 1 An exemplary layout representing the components of the instrument is shown in , except that the instrument does not include the mirror 3 or LIAD board 5 . Use a wall outlet to power the device.

[0125] Two DC power supplies (Matsusada Precision Inc., models S3-25N and S3-25P) provide ±25kV to the switching dynodes. Another DC power supply (Matsusada Precision Inc., ...

Embodiment 2

[0131] Example 2: MALDI using a portable mass spectrometer

[0132] 5 fmol, 100 fmol, or 100 pmol of angiotensin were placed on a MALDI sample plate of a portable mass spectrometer using a 2,5-dihydroxybenzoic acid matrix. Desorption-ionization was achieved using 20 laser pulses and the analytes were introduced to a quadrupole ion trap mass analyzer with an internal pressure of 2 mTorr. Figure 4 MALDI mass spectra obtained by frequency sweep are shown using each of these amounts of angiotensin ((a) 5 fmol; (b) 100 fmol; (c) 100 pmol). In each mass spectrum, the main peak is protonated angiotensin. The minimum mass of angiotensin detectable by a portable mass analyzer is 5 fmol ( Figure 4 (a)).

Embodiment 3

[0133] Example 3: ESI using a portable mass spectrometer

[0134] Pulse-mode atmospheric ESI was performed using insulin, angiotensin, cytochrome c, and myoglobin at 10% each in 45% methanol / 45% water / 10% acetic acid. -5M. For electrospray ionization, a 30 μm Picotip emitter was used with a KDS-100 syringe pump. Samples were introduced into the source using a 100 μl Hamilton syringe. The injector flow rate was 60 μl / h, the emitter voltage was 2.5 kV, and the ion introduction time was 5 s. The inlet of a 127 μm inner diameter stainless steel capillary connected to a 2-way normally closed pinch valve is controlled by a pulse signal generator; the pinch valve opens when a 24V DC signal is present. Using 1 / ±16” ID silicon tubing, connect the pinch valve and capillary. After sample introduction, the internal pressure of the quadrupole ion trap mass analyzer is reduced to 0.8 mTorr. During the scan time of 1s, from 300kHz to 100kHz Carry out a frequency scan. The ESI mass spectr...

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Abstract

Methods and apparatuses for portable mass spectrometry are disclosed. The apparatuses comprise at least one source of ionized analyte, at least one frequency scanning subsystem, at least one detector, and optionally at least one vacuum pump, and are portable. In some embodiments, the apparatuses comprise multiple sources of ionized analyte and / or are configured to obtain mass spectra of a large analyte, such as analyte with an m / z ratio of at least 105, or analyte with a molecular weight of at least 105 Da, as well as mass spectra of small molecule analyte. In some embodiments, the methods comprise obtaining mass spectra with a portable apparatus described above.

Description

[0001] This application claims the benefit of priority to US Provisional Patent Application No. 61 / 289,531, filed December 23, 2009, the entire contents of which application are incorporated herein by reference. field of invention [0002] The present invention relates to the field of mass spectrometry and, in particular, to mass spectrometry including portable instruments, including mass spectrometry using samples containing small molecule analytes or analytes with high molecular weight or mass-to-charge (m / z) ratios. Background of the invention [0003] Spectral analysis is a technique for inferring information about an analyte based on its interaction with electromagnetic fields and radiation. Mass spectrometry (MS), as its name suggests, is a measurement that involves mass. Mass spectrometers are known as the world's smallest balances because some of them can "weigh" individual atoms. Over time, the use of mass spectrometry has expanded to larger and larger molecules, i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01J49/42
CPCH01J49/0022H01J49/424H01J49/429
Inventor 陈仲瑄林俊利朱明礼
Owner ACAD SINICA
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