Preparation method and application of ecdysone-rich plant standardized extract

Abstract

The invention discloses a preparation method and an application of an ecdysone-rich plant standardized extract. The preparation method comprises the steps of: extracting ecdysone-rich plant materials using ethanol under reflux, filtering, concentrating and centrifuing; decolorizing the centrifuged supernatant, purifying by macroporous resin column chromatography, concentrating and drying to obtain the standardized extract. The method is simple and feasible, short in production period, and stable and controllable in quality, and is easy for preparation and industrial production in a standardized and batch manner; and the similarity of the extract obtained by the method is more than 99% by HPLC (high performance liquid chromatography) fingerprint spectrum detection. The extract can be applied in preparing fixed preparations serving as drugs for reducing blood sugar and resisting rheumatoid arthritis, can be used as a raw material for natural cosmetics and can be applied in animal breeding industry.

Description

Technical field The invention belongs to the field of natural product chemistry and health products, and specifically relates to a preparation method and application of a plant standard extract rich in ecdysone. Background technique Ecdysterone (ecdysterone) is a naturally occurring sterone compound, its structure is shown in Figure 1, mainly β-ecdysone (β-ecdysone). It also includes natural products with the same 6-keto-7-en-27-steroid as the basic skeleton. Ecdysone has special physiological functions and is an active and effective substance in natural medicines, health foods, and natural cosmetics. The discovery of ecdysone and its analogues in plants provides natural resources for the mass production of ecdysone. At present, most of the methods for extracting ecdysone from plants are extraction with water and heating, extraction with organic solvents such as ethyl acetate, and purification by silica gel column chromatography. Not only the extraction rate is low, but also...

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Problems solved by technology

This method only uses dew grass as raw material and uses various organic solvents for extraction, which is complicated in operation and high in cost, which is not conducive to industrial production and environmental protection

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