Rapid purification method of apolipoprotein B

A technology of apolipoprotein and purification method, which is applied in the direction of preparation method of apolipoprotein and peptide, chemical instruments and methods, etc., can solve the problems of extended production cycle, cumbersome operation, low purification efficiency, etc., and achieve reduction of purification cost, operation Simple steps and high purification yield
CN102766205AActive Publication Date: 2012-11-07广东派特埃尔生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
广东派特埃尔生物科技有限公司
Publication Date
2012-11-07

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Abstract

The invention provides a rapid purification method of apolipoprotein B, comprising the following steps of: A) taking human plasma and adding a buffering solution to be diluted and uniformly mixed; B) placing an obtained diluted plasma sample into a first water bath with the temperature of 65-75 DEG C to be heated; centrifuging and taking liquid supernatant; C) adding a surfactant with the volume percentage of 0.5-3% into the liquid supernatant and uniformly mixing to obtain processed liquid supernatant; D) placing the treated liquid supernatant into a second water bath with the temperature of50-60 DEG C to be heated; centrifuging, removing the liquid supernatant and collecting sediments; and E) washing the sediments by using a Tris-HCl buffering solution with the concentration of 0.01-0.1 mol / L for two times, and filtering to obtain purified apolipoprotein B. The rapid purification method of the apolipoprotein B, disclosed by the invention, has the advantages of being simple in operation steps, simple in needed equipment and high in purity of a purified product, and effectively reduces the purification cost.
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Description

technical field

[0001] The invention relates to a purification method of apolipoprotein, in particular to a rapid purification method of apolipoprotein B. Background technique

[0002] Apolipoprotein B is the main structural protein of low-density lipoprotein cholesterol (LDL-CHOL), accounting for about 97% of the total protein content of LDL-CHOL. The determination of apolipoprotein B can directly reflect the level of LDL-CHOL. Apolipoproteins combine with lipids to form micromicelle-like spheres, where apolipoproteins function to stabilize the sphere structure and transport lipids. The colloidal ball composed of apolipoprotein B and lipids is called low-density lipoprotein, and its diameter is 20 to 40 times that of ordinary protein. Apolipoprotein B is considered to be a risk factor for atherosclerosis, and the determination of its concentration provides a valuable indicator for the judgment and prediction of atherosclerosis and cardiovascular diseases, and has important...

Claims

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