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Complexing method and complexing product of Chinese cabbage soft-rot fungus HarpinEccs protein and zinc ions

A technology of protein and soft rot fungus, which is applied to the preparation method of peptides, chemical instruments and methods, botany equipment and methods, etc., can solve the problems of large differences in biological characteristics, achieve growth promotion, strong disease resistance, The effect of strong tolerance and storage resistance

Inactive Publication Date: 2012-11-14
吴伯骥
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are many kinds of plants, the biological characteristics of different species and varieties are quite different, the internal and external environments are also different, and the interaction between genes is different. There is a clear difference in the selectivity

Method used

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  • Complexing method and complexing product of Chinese cabbage soft-rot fungus HarpinEccs protein and zinc ions
  • Complexing method and complexing product of Chinese cabbage soft-rot fungus HarpinEccs protein and zinc ions
  • Complexing method and complexing product of Chinese cabbage soft-rot fungus HarpinEccs protein and zinc ions

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1——6, HarpinEccs zinc complex preparation:

[0021] (1) Harpin EccsThe mass ratio of protein and zinc sulfate is: between 1:1 to 6:1; (2) reaction concentration: Harpin Eccs Protein and zinc sulfate are mixed and diluted to a water content of 80% to 60%; (3) reaction pH and static treatment: adjust the pH to 6-8, and the static pretreatment time is 0-1 hour; (4) reaction temperature and time : The complexation reaction temperature is 30°C-40°C, and the complexation treatment time is 1-2 hours. During the complexation reaction, the reaction mixture needs to be stirred continuously, and the stirring speed is 100-300 revolutions per minute; (5) During the reaction, Use the EDTA method to monitor the complexation rate. After the complexation rate remains stable, it is determined by infrared spectrum KBr. If the stretching vibrations of COO(-1) and NH3(+1) are shifted to low wave numbers, it means that COO(-1) and NH3 (+1) Complex coordination with the added met...

Embodiment 7

[0026] Example 7, Harpin Eccs Stability testing of protein complexes:

[0027] The [Zn]-Harpin that we obtained by the method of embodiment 2 Eccs Protein complex and uncomplexed Harpin Eccs The stability or tolerance of proteins to various extreme conditions was compared, and the results are as follows:

[0028] At 100°C, Harpin Eccs The protein basically loses its activity after 20 minutes, while 〔Zn〕-Harpin Eccs The complex protein is still active after 40 minutes, and gradually loses its activity after 60 minutes.

[0029] Bare to the elements, Harpin Eccs After 3 days of protein electrophoresis, there is no Harpin Eccs protein band, the protein was basically disintegrated, and 〔Zn〕-Harpin Eccs After 5 days of complexing protein, [Zn]-Harpin Eccs Complexin bands are also clearly visible.

[0030] At pH 14, Harpin Eccs The protein lost its activity after 20 minutes, and 〔Zn〕-Harpin Eccs The complex protein is still active after 30 minutes and loses its activity a...

Embodiment 8

[0035] Embodiment 8, the dosage form design of protein complex:

[0036] Harpin Eccs The possible dosage forms of protein and related protein complexes in the preparation, storage and application include: the original drug includes solid or original powder and liquid or crude oil; the product dosage form for storage and application includes granules, wettable powder, water, slow Release agents and mixed preparations with other fertilizers or fungicides, insecticides, etc. Also, Harpin Eccs Related excipients and auxiliary agents for protein and related protein complex preparations, including a large amount of food-grade soluble nutrients and a small amount of key ingredients. Because this kind of protein complex and its preparation are non-toxic and pollution-free, no special protection is required during use.

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Abstract

The invention discloses a complexing method and a complexing product of Chinese cabbage soft-rot fungus HarpinEccs protein and zinc ions. Original production fungus of the HarpinEccs protein is a carrot soft-rot Erwinia carotovaora subsp. carotovora Chinese cabbage soft-rot fungus variant CSDS001 strain; the amino acid sequence of the HarpinEccs protein is shown in SEQ ID.No1; preferable complexing conditions and using ranges are given. The complexing product shows higher stability, is stronger tolerance and storability on high temperature, illumination, acid and alkaline and can be used for inducing more types of test plants subjected to allergic reaction. With the function of inducing the test plants, the test plants generally show stronger disease resistance, stronger insect prevention and other stronger stress resistance, and the complex product has the promotion function of test plant growth and development.

Description

Technical field [0001] The invention belongs to the field of biological genetic engineering, in particular to Erwinia carotovora subsp. carotovora variety CSDS001 strain hrpN Eccs The gene and its gene product, as well as the application of the gene to improve plant multifunctional activity and broad-spectrum resistance and improve plant quality through biotechnology methods. Background technique [0002] For a long time, plants have achieved co-evolution in the interaction with various living and growing environmental conditions (including biotic and abiotic) around them, and plants have formed a common adaptive ability, which is the common defense system formed in plant evolution And growth system, which is a kind of defense mechanism and growth mechanism shared by plants, and is realized by multiple signaling pathways and metabolic pathways. Studies have shown that Harpin protein, as the upstream signal substance of plants, binds to the receptor of Harpin protein widely ...

Claims

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Application Information

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IPC IPC(8): C07K14/27C07K1/00A01N47/44A01P1/00A01P3/00A01P17/00A01P21/00
Inventor 吴伯骥吴彦
Owner 吴伯骥
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