Complexing method and complexing product of Chinese cabbage soft-rot fungus HarpinEccs protein and zinc ions

A technology of protein and soft rot fungus, which is applied to the preparation method of peptides, chemical instruments and methods, botany equipment and methods, etc., can solve the problems of large differences in biological characteristics, achieve growth promotion, strong disease resistance, The effect of strong tolerance and storage resistance

Inactive Publication Date: 2012-11-14
吴伯骥
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are many kinds of plants, the biological characteristics of different species and varieties are quite different, the inter

Method used

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  • Complexing method and complexing product of Chinese cabbage soft-rot fungus HarpinEccs protein and zinc ions
  • Complexing method and complexing product of Chinese cabbage soft-rot fungus HarpinEccs protein and zinc ions
  • Complexing method and complexing product of Chinese cabbage soft-rot fungus HarpinEccs protein and zinc ions

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0020] Examples 1-6, HarpinEccs zinc complex preparation:

[0021] (1)Harpin Eccs The mass ratio of protein and zinc sulfate is between 1:1 and 6:1; (2) reaction concentration: Harpin Eccs Protein and zinc sulfate are mixed and diluted to a water content of 80% to 60%; (3) Reaction pH and standing treatment: adjust pH to 6-8, standing pretreatment time 0-1 hour; (4) Reaction temperature and time : The complexation reaction temperature is 30°C-40°C, and the complexation treatment time is 1-2 hours. During the complexation reaction, the reaction mixture needs to be continuously stirred, and the stirring speed is 100-300 revolutions per minute; (5) During the reaction, Use the EDTA method to monitor the complexation rate. After the complexation rate remains stable, it is determined by infrared spectroscopy KBr. If the extensional vibrations of COO(-1) and NH3(+1) are shifted to a low wave number, it indicates that COO(-1) and NH3 (+1) It is complexed and coordinated with the added m...

Example Embodiment

[0026] Example 7, Harpin Eccs Stability test of protein complex:

[0027] [Zn]-Harpin obtained by the method in Example 2 Eccs Protein complex and uncomplexed Harpin Eccs The stability or tolerance of the protein to various extreme conditions was compared, and the results are as follows:

[0028] At 100℃, Harpin Eccs The protein basically loses its activity after 20 minutes, and [Zn]-Harpin Eccs The complex protein is still active after 40 minutes and gradually loses activity after 60 minutes.

[0029] Exposed in natural conditions, Harpin Eccs After 3 days of protein, there is no Harpin in electrophoresis Eccs The protein band, the protein basically disintegrates, and [Zn]-Harpin Eccs After 5 days of complexing the protein, during electrophoresis [Zn]-Harpin Eccs The complex protein band is still clearly visible.

[0030] At pH14, Harpin Eccs The protein loses activity after 20 minutes, and [Zn]-Harpin Eccs The complex protein is still active after 30 minutes and loses activity after ...

Example Embodiment

[0035] Example 8. Dosage form design of protein complex:

[0036] Harpin Eccs The possible dosage forms of protein and related protein complexes in the preparation, storage and application are: original medicines include solid or raw powder and liquid or crude oil; product dosage forms for storage and application include granules, wettable powders, water, and slow Release agents and mixed preparations with other fertilizers or fungicides, insecticides, etc. In addition, Harpin Eccs Related excipients and auxiliaries for protein and related protein complex preparations, including a large number of food-grade soluble nutrients and a small amount of key ingredients. Because this kind of protein complex and its preparation are non-toxic and pollution-free, no special protection is needed when used.

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Abstract

The invention discloses a complexing method and a complexing product of Chinese cabbage soft-rot fungus HarpinEccs protein and zinc ions. Original production fungus of the HarpinEccs protein is a carrot soft-rot Erwinia carotovaora subsp. carotovora Chinese cabbage soft-rot fungus variant CSDS001 strain; the amino acid sequence of the HarpinEccs protein is shown in SEQ ID.No1; preferable complexing conditions and using ranges are given. The complexing product shows higher stability, is stronger tolerance and storability on high temperature, illumination, acid and alkaline and can be used for inducing more types of test plants subjected to allergic reaction. With the function of inducing the test plants, the test plants generally show stronger disease resistance, stronger insect prevention and other stronger stress resistance, and the complex product has the promotion function of test plant growth and development.

Description

Technical field [0001] The invention belongs to the field of biological genetic engineering, in particular to Erwinia carotovora subsp. carotovora variety CSDS001 strain hrpN Eccs The gene and its gene product, as well as the application of the gene to improve plant multifunctional activity and broad-spectrum resistance and improve plant quality through biotechnology methods. Background technique [0002] For a long time, plants have achieved co-evolution in the interaction with various living and growing environmental conditions (including biotic and abiotic) around them, and plants have formed a common adaptive ability, which is the common defense system formed in plant evolution And growth system, which is a kind of defense mechanism and growth mechanism shared by plants, and is realized by multiple signaling pathways and metabolic pathways. Studies have shown that Harpin protein, as the upstream signal substance of plants, binds to the receptor of Harpin protein widely ...

Claims

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Application Information

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IPC IPC(8): C07K14/27C07K1/00A01N47/44A01P1/00A01P3/00A01P17/00A01P21/00
Inventor 吴伯骥吴彦
Owner 吴伯骥
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