Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof

A technology of pseudomonas and screening method, applied in the fields of environmental engineering and bioengineering, can solve the problems of high cost, difficult to meet the standard, long processing period, etc., and achieve the effect of improving the denitrification effect

Active Publication Date: 2012-11-14
HIT YIXING ACAD OF ENVIRONMENTAL PROTECTION
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Problems solved by technology

[0004] Aiming at the problems that the total N in sewage treatment in winter is difficult to reach the standard, the treatment cycle is long and the cost is high, this patent screens out the strain Pseudomonas psychrophilia Den-05 which can significantly improve the efficiency of biological denitrification under low temp

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  • Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof
  • Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof

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Example Embodiment

Specific embodiment one:

[0020] (1) Take 15mL of fresh activated sludge from the Xinyigou Municipal Wastewater Treatment Plant in Harbin City and add it to a 100mL aerobic denitrification medium Erlenmeyer flask, and place it at a temperature of 10 o C, culture in a shaker with a rotation speed of 150r / min for 4~5d to obtain an enriched bacterial solution

[0021] (2) Take 15 mL of the enriched bacteria solution and add it to a 100 mL aerobic denitrification medium Erlenmeyer flask, repeat the culture conditions of step (1) 3~4 times to obtain the domesticated bacteria solution, and draw the growth curve;

[0022] (3) Dilute the domesticated bacteria liquid in a gradient, the dilution gradient is 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 , Take 100μL of the domesticated bacteria liquid and add it to the solid aerobic denitrification medium plate, spread it evenly by spreading method, and place it in an incubator at a temperature of 10℃ for 3~4 days to obtain a single colony;

[0...

Example Embodiment

[0027] Specific implementation manner 2: The strain is expanded and cultivated and then added to the SBR reactor. Method 10 o C. Centrifuge the bacteria solution for 5 minutes under the condition of 3000×g, add the bacteria to the reactor, and add 200mLOD per cycle 660 The bacterial cells obtained by centrifuging the bacterial liquid at 1.0, continuously adding the bacterial cells for 4 cycles, and determining the NO in the reactor 3 - content. The volume of the SBR reactor is 12L, the period is 8h, the precipitation time is 2h, the aeration time and the stirring time are 4h and 2h respectively, and the operation mode is aeration 2h followed by stirring for 1h. Water composition: 25.58g sodium acetate and 10g NH are contained in every 20L of water 4 Cl, 2g MgSO 4 ·7H 2 O, 2g CaCl 2 ·2H 2 O, 2g KH 2 PO 4 , 2g K 2 HPO 4 . Add a certain amount of KNO 3 , Measured NO for 20 consecutive days 3 - Concentration, data such as figure 2 .

[0028] The concentration of nitrate nitrogen i...

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Abstract

A cold-tolerant pseudomonas strain Den-05 belongs to the technical field of the biological engineering and environmental engineering, and is Pseudomonas psychrophilia Den-05, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on March 27, 2012 with the preservation number of CGMCC NO.5942. Low-temperature aerobic denitrifying bacteria Pseudomonas psychrophilia Den-05 is screened from the natural environment by a microbial pure culture technique, so that the preparation of a biological denitrifying bacterial agent is realized and a high-efficiency denitrifying bacterial agent is provided for a biological enhancement system; besides, the winter denitrification effect of an urban sewage plant is improved in the control and regulation way of shortening the period of aeration by shortening the anaerobic period and the removal of nitrate nitrogen under the aerobic condition is realized; as a result, the water quality discharged is capable of meeting the requirements.

Description

technical field [0001] The invention belongs to the technical fields of biological engineering and environmental engineering. It involves the strain Pseudomonas psychrophilia Den-05 (Pseudomonas psychrophilia Den-05) and its screening method and its application in urban sewage treatment under low temperature conditions. Background technique [0002] An appropriate amount of nitrogen in water is a basic element for synthetic algae and other microorganisms, an important material condition for the formation of an aquatic ecological environment, and the basis for a virtuous cycle of the water environment. However, at present, the total amount of nitrogen in some water bodies in my country has exceeded the capacity of the water body. Excessive nitrogen causes the abnormal proliferation of algae and other organisms in the water body, causing water quality to deteriorate. At the same time, ammonia nitrogen and nitrite nitrogen are toxic to fish and other organisms. Since the vari...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/02C02F3/34C12R1/38
CPCY02W10/10
Inventor 马放庞长泷崔迪李昂邱天孔祥震蔡蕊
Owner HIT YIXING ACAD OF ENVIRONMENTAL PROTECTION
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