Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof
A technology of pseudomonas and screening method, applied in the fields of environmental engineering and bioengineering, can solve the problems of high cost, difficult to meet the standard, long processing period, etc., and achieve the effect of improving the denitrification effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
Specific embodiment one:
[0020] (1) Take 15mL of fresh activated sludge from the Xinyigou Municipal Wastewater Treatment Plant in Harbin City and add it to a 100mL aerobic denitrification medium Erlenmeyer flask, and place it at a temperature of 10 o C, culture in a shaker with a rotation speed of 150r / min for 4~5d to obtain an enriched bacterial solution
[0021] (2) Take 15 mL of the enriched bacteria solution and add it to a 100 mL aerobic denitrification medium Erlenmeyer flask, repeat the culture conditions of step (1) 3~4 times to obtain the domesticated bacteria solution, and draw the growth curve;
[0022] (3) Dilute the domesticated bacteria liquid in a gradient, the dilution gradient is 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 , Take 100μL of the domesticated bacteria liquid and add it to the solid aerobic denitrification medium plate, spread it evenly by spreading method, and place it in an incubator at a temperature of 10℃ for 3~4 days to obtain a single colony;
[0...
Example Embodiment
[0027] Specific implementation manner 2: The strain is expanded and cultivated and then added to the SBR reactor. Method 10 o C. Centrifuge the bacteria solution for 5 minutes under the condition of 3000×g, add the bacteria to the reactor, and add 200mLOD per cycle 660 The bacterial cells obtained by centrifuging the bacterial liquid at 1.0, continuously adding the bacterial cells for 4 cycles, and determining the NO in the reactor 3 - content. The volume of the SBR reactor is 12L, the period is 8h, the precipitation time is 2h, the aeration time and the stirring time are 4h and 2h respectively, and the operation mode is aeration 2h followed by stirring for 1h. Water composition: 25.58g sodium acetate and 10g NH are contained in every 20L of water 4 Cl, 2g MgSO 4 ·7H 2 O, 2g CaCl 2 ·2H 2 O, 2g KH 2 PO 4 , 2g K 2 HPO 4 . Add a certain amount of KNO 3 , Measured NO for 20 consecutive days 3 - Concentration, data such as figure 2 .
[0028] The concentration of nitrate nitrogen i...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2023 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap