Manufacture method and application of silver hybridization SBA-15 electrochemical luminescence immunosensor

A technology of luminescence immunity and electrochemistry, which is applied in the fields of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis through chemical reactions of materials, etc. problem, to achieve the effect of improving electron transfer efficiency, excellent synergistic sensitization effect, and short response time

Inactive Publication Date: 2012-11-14
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1. Radioimmunoassay: It has high sensitivity and accuracy, and the reaction conditions are generally required, but the reagents used in this technique are radioactive and have certain harm to the human body
[0007] 2. Enzyme-linked immunoassay: It has high sensitivity and the equipment used is s

Method used

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  • Manufacture method and application of silver hybridization SBA-15 electrochemical luminescence immunosensor
  • Manufacture method and application of silver hybridization SBA-15 electrochemical luminescence immunosensor
  • Manufacture method and application of silver hybridization SBA-15 electrochemical luminescence immunosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] (1) Preparation of AgSBA-15 nanocomposite, the steps are as follows:

[0052] 1) Preparation of silver nanoparticles: add 48mL ultrapure water to the Erlenmeyer flask, add 1mL 50mmol / L AgNO under stirring 3 solution and 1 mL of 5% sodium citrate solution. Then, in above-mentioned solution, add a small amount of sodium borohydride solid, continue stirring 10min, solution turns brownish yellow, shows to form silver nanoparticle. Continue stirring until the color of the silver nanoparticle colloidal solution no longer changes. Centrifuge and store the supernatant in the dark;

[0053] 2) Preparation of AgSBA-15 nanocomposite material: Take 2mL silver nanoparticle colloidal solution and 4mg aminated SBA-15, shake for 24h, centrifuge, remove the supernatant to obtain AgSBA-15.

[0054] (2) Ru(bpy) 3 2+ -The preparation of AgSBA-15, the steps are as follows:

[0055] 5mg of sodium dodecylsulfonate was ultrasonically dissolved in 3mL of ultrapure water, AgSBA-15 was adde...

Embodiment 2

[0064] (1) Preparation of AgSBA-15 nanocomposite, the steps are as follows:

[0065] 1) Preparation of silver nanoparticles: add 48mL ultrapure water to the Erlenmeyer flask, add 1mL 50mmol / L AgNO under stirring 3 solution and 1 mL of 5% sodium citrate solution. Then, a small amount of sodium borohydride solid was added to the above solution, and the stirring was continued for 10 min, and the solution turned brownish yellow, indicating the formation of silver nanoparticles. Continue stirring until the color of the silver nanoparticle colloidal solution no longer changes. Centrifuge and store the supernatant in the dark;

[0066] 2) Preparation of AgSBA-15 nanocomposite material: take 2mL of silver nanoparticle colloidal solution and 5mg of aminated SBA-15, shake for 24h, centrifuge, remove the supernatant to obtain AgSBA-15.

[0067] (2) Ru(bpy) 3 2+ -The preparation of AgSBA-15, the steps are as follows:

[0068] 10 mg of sodium dodecyl sulfonate was ultrasonically diss...

Embodiment 3

[0077] (1) Preparation of AgSBA-15 nanocomposite, the steps are as follows:

[0078] 1) Preparation of silver nanoparticles: add 48mL ultrapure water to the Erlenmeyer flask, add 1mL 50mmol / L AgNO under stirring 3 solution and 1 mL of 5% sodium citrate solution. Then, a small amount of sodium borohydride solid was added to the above solution, and the stirring was continued for 10 min, and the solution turned brownish yellow, indicating the formation of silver nanoparticles. Continue stirring until the color of the silver nanoparticle colloidal solution no longer changes. Centrifuge and store the supernatant in the dark;

[0079] 2) Preparation of AgSBA-15 nanocomposite material: take 2mL silver nanoparticle colloidal solution and 6mg aminated SBA-15, shake for 24h, centrifuge, remove the supernatant to obtain AgSBA-15.

[0080] (2) Ru(bpy) 3 2+ -The preparation of AgSBA-15, the steps are as follows:

[0081] 15mg of sodium dodecylsulfonate was ultrasonically dissolved in...

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Abstract

The invention relates to a manufacture method and application of a silver hybridization SBA-15 electrochemical luminescence immunosensor. Ru(bpy)32+ is used as a luminescent material, a mesoporous nanometer material SBA-15 has large specific surface area and good biological compatibility and can be used as a substrate material fixing and carrying the Ru(bpy)32+ and tumor marker antibody, an anionic surfactant of sodium dodecyl sulfate is wrapped on the surface of the SBA-15, more Ru(bpy)32+ is absorbed through electrostatic interaction, silver nano particles are hybridized in the SBA-15, and luminescence of the Ru(bpy)32+ is promoted. The electrochemical luminescence immunosensor for a tumor marker has the advantages of being low in cost, high in sensitivity, good in specificity, fast in detecting and simple in preparation process and is applicable to fast detecting of various tumor markers in serum.

Description

technical field [0001] The invention relates to the preparation and application of silver hybridized SBA-15 electrochemiluminescence immunosensor. Specifically, the silver-hybridized mesoporous SBA-15 (AgSBA-15 composite material) is used to prepare a label-free electrochemiluminescent immunosensor for detecting various tumor markers, which belongs to the technical field of new functional materials and biosensing detection. Background technique [0002] Tumor markers are a class of biochemical substances produced by tumor tissue itself that can reflect the existence and growth of tumors. [0003] They may not exist in normal adult tissues but are only found in embryonic tissues, or their content in tumor tissues greatly exceeds that in normal tissues. Their existence or quantitative changes can indicate the nature of tumors, so as to understand the histogenesis and cell differentiation of tumors , Cell function, thereby helping the diagnosis, classification, prognosis judgm...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N27/327G01N27/36
Inventor 魏琴李小建李贺张勇于海琴吴丹朱宝存杜斌李玉阳罗川南曹伟马洪敏
Owner UNIV OF JINAN
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