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Strain of endophytic Streptomyces lydicus DOR3-2 of Chinese yam and its application

A technology of Streptomyces lidi and yam, applied in the application, bacteria, biocide and other directions, can solve the problems such as yam anthracnose that have not yet been seen, and achieve the effect of broad-spectrum anti-filamentous fungi activity and good control effect.

Inactive Publication Date: 2013-11-27
HAINAN UNIVERSITY
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Problems solved by technology

There have been some successful reports on the use of plant endophytic actinomycetes for plant disease control, but there is no research report on the use of yam endophytic actinomycetes to control yam anthracnose

Method used

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  • Strain of endophytic Streptomyces lydicus DOR3-2 of Chinese yam and its application
  • Strain of endophytic Streptomyces lydicus DOR3-2 of Chinese yam and its application
  • Strain of endophytic Streptomyces lydicus DOR3-2 of Chinese yam and its application

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Experimental program
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Effect test

Embodiment 1

[0035] The isolation culture method of embodiment one Streptomyces lydidi DOR3-2

[0036] 1. Take a healthy yam root sample, rinse it with tap water, dry it at room temperature, cut it into 1-2 cm small pieces, rinse it with 0.1% Tween 20 for a few seconds, disinfect it with 5% sodium hypochlorite for 10 minutes, and disinfect it with 2.5% sterile Na 2 S 2 o 3 Wash with solution for 10 minutes, disinfect with 70% alcohol for 5 minutes, rinse with sterile water twice, and disinfect with 10% sterile NaHCO 3 Wash the solution for 10 minutes, and after air-drying, add a small amount of sterile water and quartz sand to grind the tissue into a homogenate with a sterile mortar,

[0037] 2. After filtering the homogenate with two layers of gauze, dilute it 10, 100 times, take 0.2 ml of each dilution and spread it on the IMA-2 separation medium, and culture it at 28°C for 1-4 weeks.

[0038] 3. According to the culture characteristics of the strains on the isolation medium, pick a s...

Embodiment 2

[0040] Embodiment two Streptomyces lydidis DOR3-2 plate confrontation anti-filamentous pathogenic fungus test

[0041] 1. Inoculate Streptomyces lydida DOR3-2 on the ISP2 plate and cultivate it in a 28°C incubator for 1 week, and inoculate the pathogenic filamentous fungus on the PDA medium for 1 week in a 28°C incubator.

[0042] 2. Use an inoculation loop to pick a single actinomycetes colony block with a diameter of 2 mm and inoculate it on the side of the PDA medium solid medium (1.5 cm away from the center of the plate), and place it in a 28°C incubator for 3-4 days.

[0043] 3. When the actinomycete colony grows to the size of mung bean, inoculate a filamentous fungal mycelium block with a diameter of 6 mm at a distance of 3 cm from the actinomycete (the two inoculation points are centrally symmetrical). The strains not inoculated with actinomycetes were used as the control, and each strain was repeated three times. After culturing at 28°C for 5-7 days, the distance betw...

Embodiment 3

[0048] Example 3 Anti-Yam Anthracnose Test of Streptomyces lydidis DOR3-2 Back-grafted with Chinese Yam Tissue Culture Seedlings

[0049] 1. Inoculate Streptomyces lydida DOR3-2 on the ISP2 plate and culture it in an incubator at 28°C for 1 week, scrape the colony of Streptomyces lydida DOR3-2 from the plate with a sterile knife, and wash it with sterile water to make Mycelia or spore suspension (3-4×10 6 cfu / ml).

[0050] 2. Take 1-2ml of bacterial suspension and inject it on the surface of the growth medium of yam tissue culture seedlings, use sterile water as a control, cultivate in the light incubator for 14 days, take out the stems and leaves of the tissue culture seedlings, and cut them under aseptic conditions. into small pieces, then inoculated into the solid medium of IMA-2 medium, and cultured for 1-4 weeks to observe whether the actinomycetes grew out of the tissue pieces, and judge whether the actinomycetes were successfully inoculated with the yam tissue culture ...

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Abstract

The invention discloses a strain of endophytic Streptomyces lydicus DOR3-2 of Chinese yam and application of the same in biological control of anthracnose of Chinese yam. The strain of endophytic Streptomyces lydicus DOR3-2 of Chinese yam provided by the invention is preserved in China Center for Type Culture Collection with an accession number of CCTCC NO: M2012237 and is prepared by carrying out isolation and culture on roots of Chinese yam. The Streptomyces lydicus DOR3-2 provided by the invention has a broad spectrum of activity against filamentous fungi and a good control effect on Dioscorea lianas in Dioscoreaceae, particularly on anthracnose of Chinese yam, and provides technical supports for mass production of Chinese yam and an effective control approach for biological control of Chinese yam.

Description

technical field [0001] The invention relates to a yam endophytic Streptomyces lydidae and its application, in particular to a yam endophytic Streptomyces lydida DOR3-2 for the biological control of yam anthracnose and its application. Background technique [0002] Yam (Dioscorea opposita Thunb.), also known as yam and yam, is a vine of the genus Dioscorea in the family Dioscorea, and is a traditional Chinese medicinal material in my country. It has pharmacological effects such as treating kidney deficiency, high blood pressure, diabetes, asthma, neurasthenia and low back pain. Yam is rich in nutrition, unique in flavor, good in taste, and does not contain any harmful substances. It is the king of green health food in the world today and one of the important specialty export vegetables in my country. China's tropical and subtropical regions are the origin and domestication center of yam, and it is currently cultivated in most parts of our country. In recent years, with the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/465
Inventor 黄小龙周双清黄东益胡新文
Owner HAINAN UNIVERSITY
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