A kind of method for producing yeast ribonucleic acid
A yeast ribonucleic acid, yeast technology, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve problems such as unfavorable 5'-nucleotide preparation, dark nucleic acid color, affecting yield, etc. The drying process time is shortened, the acid-base wastewater is reduced, and the solubility is excellent.
Active Publication Date: 2016-12-21
青岛大波高科生物有限公司
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Problems solved by technology
On the one hand, the use of dilute alkali method produces a large amount of alkali-containing wastewater in the production process, and it needs to consume a larger amount of acid than the concentrated salt method in the subsequent isoelectric point acid precipitation link. More importantly, many " Nucleic acid with the absence of "headless" phosphorus terminal is not conducive to the preparation of 5'-nucleotides in the nucleotide industry, which has the greatest demand for nucleic acids, and the color of dilute alkali nucleic acid is quite dark
The dilute alkali method needs to be very careful. The alkali can easily break the ester bond in the ribonucleic acid molecule and become 2', 3'-nucleotide, which will degrade the ribonucleic acid and affect the yield.
Method used
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Embodiment 1
[0041] Select the yeast mud within the 5th generation of the brewery, and transport it to the workshop with a heat-insulated tanker within 10°C. Within 5 hours, use the drum drying or spray drying process to dry the beer yeast mud to make beer yeast powder, and put it in the feeding tank with water at room temperature. The yeast powder is formulated into a 10% yeast powder slurry, and the pH of the slurry is adjusted to about 3.0 with 6mol / L hydrochloric acid, and left to stand for 30 minutes.
[0042] Then use 6mol / L sodium hydroxide to adjust the pH of the slurry to 7.0, and the plate heat exchanger (Shanghai Hutao Heat Exchange Equipment Co., Ltd., model BR6) instantly raises the temperature of the material to 95 degrees, and keeps it warm in an extraction tank containing a steam jacket. Extract for 2 hours.
[0043] After the extraction is completed, use a plate heat exchanger (internal flow of ice and brine) to exchange the heat of the material to about 10°C, and adjust t...
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The invention provides a method for producing a ribonucleic acid of yeast. A ribonucleic acid extraction process of the method does not utilize sodium chloride and ammonium chloride to change osmotic pressure of a cell, and does not utilize an alkali material such as high-concentration sodium hydroxide to extract a ribonucleic acid. A purification process of the method utilizes two pH adjustment processes to remove most of acidic proteins and basic proteins, utilizes an activated clay adsorbent to adsorb hybrid proteins and polysaccharides, and re-utilizes isoelectric points to precipitate a ribonucleic acid. Ribonucleic acid-free yeast powder obtained by ribonucleic acid extraction has low salt content and is suitable as feed. Acid-alkali wastewater produced in production is reduced obviously. A ribonucleic acid produced by the method has high purity, a very light ivory color and an integrated end structure, can be used for preparation of 5'-nucleotide used for nucleic acid downstream industries, and also can be used for preparation of a high-purity sodium ribonucleate which has excellent solubility, can be dissolved at a low temperature and is transparent after being dissolved.
Description
technical field [0001] The present invention relates to a method for producing yeast ribonucleic acid. Background technique [0002] Ribonucleic acid (RNA) is ubiquitous in animal, plant and microbial cells, and is closely related to protein biosynthesis. Microbial-derived RNA and its degradation product nucleotides have important application value in the fields of medicine, food, health care products and agriculture. Yeast nucleic acid, also known as yeast RNA, is ribonucleic acid extracted from natural yeast. Due to the different species of yeast, the nucleic acid (RNA) contained in it varies from 3-15%. Nucleic acid is an important genetic material, which is of great significance to living organisms. Therefore, in recent years, some large companies producing yeast at home and abroad, They have successively relied on their own yeast resources to carry out deep processing and comprehensive utilization of yeast to produce yeast nucleic acid products. [0003] There are ma...
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IPC IPC(8): C12N15/10C07H21/02C07H1/08
Inventor 楚大波
Owner 青岛大波高科生物有限公司