Breeding method of avermectin strains

Abstract

The invention relates to a breeding method of avermectin strains. The breeding method comprises the following steps: primarily screening by an agar block culture method, namely slant culture of a seed lot, preparing single spore suspension, coating, separating and cultivating a single bacterial colony; picking the single colony to be dibbled on a solid culture medium plate and picking the single bacterial colony to carry out slant culture; detecting pigment-producing capacity by an HPLC (high performance liquid chromatography) method as a primary screening titer after the bacterial colony to be dibbled is cultured maturely; picking a bevel bacterial colony flask which is higher than the primary screening titer to secondarily screen; detecting a secondary screen flask titer by the HPLC method, and secondarily screening a high-yielding strain and reserving seed for planting; inspecting the performance of the high-yielding strain; and finally reserving the seed for planting. According to the breeding method of the avermectin strains, the breeding cycle of the avermectin strain can be effectively shortened; the screening efficiency is improved, and the screening cost is saved. Compared with the traditional primarily screening and breeding method of the avermectin flask, the screening period can be reduced by about half a month, and the strain performance obtained by an agar block culture primary screening method effectively corresponds to a verification result obtained by corresponding proliferation slant.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation medicine, in particular to a method for breeding abamectin strains. Background technique [0002] Abamectin is a group of insecticidal antibiotics produced by Streptomyces avermanii, composed of a sixteen-membered macrolide and a dioleanose, which is used in the prevention and treatment of agricultural diseases and insect pests and animal husbandry parasitic diseases It has a unique effect and has been widely used in our country, and it is the second largest pesticide variety exported by our country. [0003] At present, in the breeding of abamectin strains in my country, the quality of its performance is judged by the titer of antibiotics produced by the shake flask culture fermentation liquid. Abamectin screening natural breeding process in the prior art is as follows: select starting strain, prepare separation plate culture medium, prepare single spore suspension, spread and is...

AI Technical Summary

Problems solved by technology

Because abamectin has no antibacterial activity, it cannot secrete substances that inhibit the growth of the test bacteria or secrete certain substances that are toxic to the test bacteria. When it is transferred to the medium containing the test bacteria, it will be inhibited or even suppressed by the test bacteria. Therefore, the detection of the potency of abamectin in the selection process cannot use the zone of inhibition method

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