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Chip, preparation method, application and method for screening drugs

A chip and selected technology, applied in the fields of drug screening, chip, and preparation, can solve the problems of immobilization of various small molecules and lack of connecting arms

Active Publication Date: 2015-07-08
苏州普芯生命科学技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this specific immobilization strategy is very suitable for immobilizing synthetic compounds, the structures of natural compounds are usually diverse, and it is difficult to immobilize a variety of small molecules on the same chip with a single immobilization method, especially some circular small molecules and without connecting arm for fixing
Therefore, immobilization of natural compounds is a technical bottleneck for small molecule arrays

Method used

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  • Chip, preparation method, application and method for screening drugs
  • Chip, preparation method, application and method for screening drugs
  • Chip, preparation method, application and method for screening drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0152] Embodiment 1 The preparation of the chip provided by the present invention

[0153] Evaporate or magnetron sputter a 1-2nm chromium film on the surface of a clean glass substrate, and then evaporate or magnetron sputter an about 50nm gold film on the surface of the chromium film.

[0154] Take 30mL of distilled water and heat it to 70~80°C, then add 6mL of ammonia water and 6mL of hydrogen peroxide; put the gold-plated glass substrate prepared above, and treat it at 70~80°C for 10 minutes; after cooling for 10 minutes, wash it with distilled water and ethanol, and dry it with nitrogen , to produce a solid support.

[0155] The dithiol initiator (such as the compound shown in formula VI) was mixed with the diluent mercaptopolyethylene glycol at a molar ratio of 1:20 to prepare the self-assembly initiating compound, with ethanol as the solvent, and the total concentration was 1 mM. The solid support prepared above was soaked in the self-assembly initiating compound solut...

Embodiment 2

[0158] Embodiment 2 Preparation of the chip provided by the present invention

[0159] Evaporate or magnetron sputter a 1-2nm chromium film on the surface of a clean glass substrate, and then evaporate or magnetron sputter an about 50nm gold film on the surface of the chromium film.

[0160] Take 30mL of distilled water and heat it to 70~80°C, then add 6mL of ammonia water and 6mL of hydrogen peroxide; put the gold-plated glass substrate prepared above, and treat it at 70~80°C for 10 minutes; after cooling for 10 minutes, wash it with distilled water and ethanol, and dry it with nitrogen , to produce a solid support.

[0161] The dithiol initiator (such as the compound shown in formula VII) was mixed with the diluent mercaptopolyethylene glycol at a molar ratio of 1:2000 to prepare the self-assembly initiating compound, with ethanol as the solvent, and the total concentration was 1mM. The solid support prepared above was soaked in the self-assembly initiating compound solutio...

Embodiment 3

[0164] Embodiment 3 Preparation of the chip provided by the present invention

[0165] Evaporate or magnetron sputter a 1-2nm chromium film on the surface of a clean glass substrate, and then evaporate or magnetron sputter an about 50nm gold film on the surface of the chromium film.

[0166] Take 30mL of distilled water and heat it to 70~80°C, then add 6mL of ammonia water and 6mL of hydrogen peroxide; put the gold-plated glass substrate prepared above, and treat it at 70~80°C for 10 minutes; after cooling for 10 minutes, wash it with distilled water and ethanol, and dry it with nitrogen , to produce a solid support.

[0167] The dithiol initiator (such as the compound shown in formula VIII) was mixed with the diluent mercaptopolyethylene glycol at a molar ratio of 1:200 to prepare the self-assembly initiating compound, with ethanol as the solvent and a total concentration of 1 mM. The solid support prepared above was soaked in the self-assembly initiating compound solution a...

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Abstract

Disclosed is a chip and preparation method thereof and method for screening drug, and the chip comprises a solid support, an initiating composition, an initiating monomer and a photo-crosslinker. The preparation method comprises obtaining initiating composition by mixing initiator and diluent, and obtaining a high-molecular polymer by polymerizing the initiating monomer of the initiating composition after pretreating the solid support, and activating the high-molecular polymer to react and couple with the photo-crosslinker. The method for screening a drug comprises mixing the substance to be examined with the chip, and obtaining the first response value by surface plasmon resonance detection, and mixing the negative control with the chip to obtain the second response value for detection, and judging whether the substance to be examined is combined with the target by comparing the two values. The chip has a good capacity for resisting nonspecific protein adsorption, and can immobilize a variety of small molecular substances in parallel, and can be used for screening drugs having complicated ingredients.

Description

technical field [0001] The invention relates to the field of drug screening, in particular to a chip, a preparation method, use and a method for screening drugs. Background technique [0002] Traditional Chinese medicine is the cultural precipitation of our country for thousands of years, and has a long history and cultural origin. However, the development of traditional Chinese medicine still relies on traditional methods for many years and is based on raw materials, lacking systematic modern scientific basic research. The mechanism of action of most traditional Chinese medicines is unclear, the key components of traditional Chinese medicine prescriptions are not clear, and there may be unexpected side effects. These factors have hindered traditional Chinese medicine from entering the mainstream of the international natural medicine market. In order for traditional Chinese medicine to go global and enter the international market, it must realize the modernization of tradi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/15G01N21/552
CPCG01N33/54373G01N21/553G01N33/54353
Inventor 朱劲松何建安陈新颖彭开美
Owner 苏州普芯生命科学技术有限公司
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