Lactic acid bacteria used for silage corn and use method thereof

A technology of lactic acid bacteria and corn silage, applied in the field of Lactobacillus for silage, can solve the problems of affecting the quality of silage, secondary fermentation, loss of nutrients and the like

Active Publication Date: 2013-01-09
BEIJING SCITOP BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whole corn silage or corn stalk silage is currently the most common silage, which accounts for more than 90% of the silage. However, the conventional corn silage has a problem of success rate. In the silage process, there are insufficient lactic acid bacteria, acidification The loss of nutrients caused by too slow speed, as well as the secondary fermentation after opening the cellar, all directly or indirectly affect the quality of silage

Method used

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  • Lactic acid bacteria used for silage corn and use method thereof
  • Lactic acid bacteria used for silage corn and use method thereof
  • Lactic acid bacteria used for silage corn and use method thereof

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Experimental program
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Effect test

Embodiment 1

[0020] A strain of Lactobacillus plantarum, said Lactobacillus plantarum (L.plantarum Ps-8) is a lactic acid bacterium with good acid resistance, strong growth ability and fast acid production speed screened out from 130 strains of Lactobacillus plantarum; It has been preserved in the General Microorganism Culture Collection Center of China Microbiology Culture Collection Management Committee, and the preservation number is CGMCC No.5359.

[0021] Application of the plant lactobacillus in corn stalk silage.

[0022] Strain activation: inoculate frozen L. plantarum Ps-8 in MRS liquid medium, culture at 37°C for 18-22 hours, and subculture 2-3 times in this way to obtain the activated L. plantarum Ps-8 bacteria The composition of the MRS liquid medium is as follows: 10g peptone, 5g beef extract, 4g yeast extract powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1mL Tween 80, 0.2g Add magnesium sulfate and 0.05g manganese sulfate to...

Embodiment 2

[0030] After centrifuging the activated strains, add an appropriate amount of sterilized water to make a suspension of bacteria so that the number of live lactic acid bacteria is 1×10 8 cfu / mL. Take 1×10 5 The inoculation amount of cfu / g was inoculated into the corn raw material, and the same volume of sterilized distilled water was added as a control group. After being vacuum-packed by a vacuum packaging machine, it was placed in a storage room for fermentation. After 30 days of fermentation, that is, after the fermentation is mature, a part of the silage is taken out for sensory evaluation, microbial composition and fermentation quality analysis.

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Abstract

The invention discloses a corn silage lactobacillus plantarum, which can quicken corn to be ensiled and mature and improve the quality of the corn silage feed. The lactic acid bacteria used for the silage corn has the technical scheme that the lactobacillus plantarum (Lactobacillus plantarum Ps-10) is lactic acid bacteria which is screened from 130 plants of lactobacillus plantarum and has the advantages of favorable acid resistance, strong growth capability and high acid production speed. The bacterial strain of the bacteria is collected in the China general microbiological culture collection center with the collection number of CGMCC (China general microbiological culture collection center) No. 5359, and the collection data is 28th, Oct., 2011. The invention also discloses a use method for the lactic acid bacteria.

Description

technical field [0001] The invention relates to a strain of Lactobacillus for silage, in particular to a strain of Lactobacillus for corn silage plant capable of accelerating the maturation of corn silage and improving the quality of corn silage and its application method. Background technique [0002] Silage is a storage method that quickly reduces the pH value through lactic acid fermentation and maintains an anaerobic environment to facilitate the long-term preservation of silage crops. Compared with forage grass drying into hay, forage silage can greatly shorten the time from harvesting to storage, avoid the loss of forage grass caused by bad weather, and preserve the nutrients of green forage grass to the maximum extent. Whole corn silage or corn stalk silage is currently the most common silage, which accounts for more than 90% of the silage. However, the conventional corn silage has a problem of success rate. In the silage process, there are insufficient lactic acid ba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K3/03C12R1/25
Inventor 于洁高鹏飞
Owner BEIJING SCITOP BIO TECH CO LTD
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