Method for simultaneously preparing ferulic acid, xylo-oligosaccharides and ethanol from spent grains

A technology of xylo-oligosaccharide and ferulic acid, which is applied in the field of xylo-oligosaccharide and ethanol, and the preparation of ferulic acid at the same time, can solve the problems of high consumption of chemical reagents, secondary pollution, etc., and achieve environmental friendliness and low consumption of chemical reagents. , the effect of simple preparation process

Inactive Publication Date: 2013-01-09
HUAQIAO UNIVERSITY
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Problems solved by technology

However, these preparation methods all use pure chemical methods, which require high consumption of chemical reagents and easily cause secondary pollution.

Method used

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Examples

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Embodiment

[0018] (1) Preparation of ethanol-insoluble matter of wheat grains: first immerse 100g of wheat grains in ethanol with a volume fraction of 85% and boil for 5 minutes, then cool and filter, repeat twice; then wash and filter the grains with absolute ethanol and ether in turn The wheat grains were dried; then the dried wheat grains were pulverized with a bead mill and passed through a 50-mesh soil sample sieve to obtain 81 g of ethanol-insoluble matter of the wheat grains;

[0019] (2) Preparation of ferulic acid and xylo-oligosaccharides: Weigh 10g of the ethanol-insoluble matter of wheat grains obtained in step (1), add 80mL of 0.1mol / L NaOH solution, soak for 24h, and then use a power of 264w After microwave treatment for 20 minutes, centrifuge to obtain 68mL of centrifuge (that is, the solution after centrifugation). Adjust the pH value of the centrifuge to 5.0 and cool it to 45°C. After reacting with 3400U crude xylanase solution for 12h, add 2720mU The ferulic acid estera...

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Abstract

The invention provides a method for simultaneously preparing ferulic acid, xylo-oligosaccharides and ethanol from spent grains. The method comprises the following steps: soaking spent grains in 85 vol.% ethanol to boil for 5 minutes, cooling and filtering twice; washing the filtered spent grains with absolute alcohol and ethylether in turn, drying, grinding, sieving with a 50 meshes of sieve to obtain the ethanol insolubles of spent grains; and then soaking the ethanol insolubles of spent grains in a 0.1mol / L NaOH solution, performing microwave treatment, centrifuging, then using feruloyl esterase and xylanase to treat and obtain ferulic acid and xylo-oligosaccharides, and saccharifying and fermenting the treated ethanol insolubles of spent grains in turn to finally obtain ethanol. By adopting the method, the consumptions of the chemical reagents are low, thus the aim of environmental protection can be realized; and the three main components of wood fiber can be utilized simultaneously.

Description

【Technical field】 [0001] The invention relates to a preparation method in the field of biochemical industry, in particular to a method for simultaneously preparing ferulic acid, xylooligosaccharides and ethanol from wheat grains. 【Background technique】 [0002] At home and abroad, the biotransformation research of plant cellulosic raw materials has made gratifying progress, but the three main components of plant cellulosic materials have not been fully and comprehensively utilized, often for the purpose of producing a single product, and the economic benefits are low. For example, the use of plant cellulose to produce fuel ethanol, xylose, xylitol, furfural, etc. only uses one of the components, while ignoring the use of other components; due to the limited value of these single products, bio-based products Cannot compete with petroleum based products. The purpose of the current pretreatment method is more to consider the utilization of cellulose in lignocellulosic raw mate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/42C12P19/14C12P7/10C12R1/865
CPCY02E50/16Y02E50/10
Inventor 李夏兰方柏山程珊影张兰
Owner HUAQIAO UNIVERSITY
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