Method for simultaneously preparing ferulic acid, xylo-oligosaccharides and ethanol from spent grains

Abstract

The invention provides a method for simultaneously preparing ferulic acid, xylo-oligosaccharides and ethanol from spent grains. The method comprises the following steps: soaking spent grains in 85 vol.% ethanol to boil for 5 minutes, cooling and filtering twice; washing the filtered spent grains with absolute alcohol and ethylether in turn, drying, grinding, sieving with a 50 meshes of sieve to obtain the ethanol insolubles of spent grains; and then soaking the ethanol insolubles of spent grains in a 0.1mol / L NaOH solution, performing microwave treatment, centrifuging, then using feruloyl esterase and xylanase to treat and obtain ferulic acid and xylo-oligosaccharides, and saccharifying and fermenting the treated ethanol insolubles of spent grains in turn to finally obtain ethanol. By adopting the method, the consumptions of the chemical reagents are low, thus the aim of environmental protection can be realized; and the three main components of wood fiber can be utilized simultaneously.

Description

【Technical field】 [0001] The invention relates to a preparation method in the field of biochemical industry, in particular to a method for simultaneously preparing ferulic acid, xylooligosaccharides and ethanol from wheat grains. 【Background technique】 [0002] At home and abroad, the biotransformation research of plant cellulosic raw materials has made gratifying progress, but the three main components of plant cellulosic materials have not been fully and comprehensively utilized, often for the purpose of producing a single product, and the economic benefits are low. For example, the use of plant cellulose to produce fuel ethanol, xylose, xylitol, furfural, etc. only uses one of the components, while ignoring the use of other components; due to the limited value of these single products, bio-based products Cannot compete with petroleum based products. The purpose of the current pretreatment method is more to consider the utilization of cellulose in lignocellulosic raw mate...

AI Technical Summary

Problems solved by technology

However, these preparation methods all use pure chemical methods, which require high consumption of chemical reagents and easily cause secondary pollution.