Detection method for twelve kinds of pyrethroids pesticide residues in capsanthin
A technology for pyrethroids and detection methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to achieve effective detection, matrix interference, complex matrix, etc., and achieve easy mass sample detection, high accuracy, The effect of reducing interference
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Embodiment 1
[0020] Embodiment 1: the specific implementation steps of the detection method of twelve kinds of pyrethroid pesticide residues in this capsanthin are as follows:
[0021] (1) Standard curve: Precisely measure an appropriate amount of standard stock solutions of twelve pyrethroid pesticide residues, and dilute them with Fisher chromatography-pure hexane stepwise to a mass concentration of 0.01, 0.05, 0.1, 0.2, 0.5, and 1.0 mg, respectively. / L series of standard solutions were injected and measured according to the above test conditions, and the peak areas Y of twelve pyrethroid pesticide residues were plotted against their mass concentrations X respectively.
[0022] (2) Sample extraction: accurately weigh 1.0g (accurate to 0.0001g) =200 capsanthin samples were placed in a 25mL volumetric flask, 12.5mL of cyclohexane-ethyl acetate mixed solvent with a volume ratio of 50:50 was added, ultrasonicated for 15min, twice for 2 times, cooled to room temperature, and added with a vo...
Embodiment 2
[0031] Embodiment 2: the specific implementation steps of the detection method of twelve kinds of pyrethroid pesticide residues in this capsanthin are as follows:
[0032] (1) Standard curve: same as Example 1.
[0033] (2) Sample extraction: accurately weigh 0.5g (accurate to 0.0001g) =50 capsanthin samples were placed in a 10mL volumetric flask, 10mL of cyclohexane-ethyl acetate mixed solvent with a volume ratio of 50:50 was added, ultrasonicated for 10min, cooled to room temperature, and cyclohexane-acetic acid with a volume ratio of 50:50 was added Dilute ethyl ester mixed solvent to volume. The extract was transferred to a centrifuge tube, centrifuged at 4000 r / min for 5 min at 5°C, the supernatant was filtered with a 0.45 μm filter membrane, and the filtrate was transferred to a GPC sample bottle.
[0034] (3) GPC purification treatment: The GPC detection wavelength was 254 nm, the mobile phase was a cyclohexane-ethyl acetate mixed solvent with a volume ratio of 50:50...
Embodiment 3
[0039] Embodiment 3: the specific implementation steps of the detection method of twelve kinds of pyrethroid pesticide residues in this capsanthin are as follows:
[0040] (1) Standard curve: same as Example 1.
[0041] (2) Sample extraction: accurately weigh 1.5g (accurate to 0.0001g) of =50 capsanthin samples were placed in a 25mL volumetric flask, 25mL of cyclohexane-ethyl acetate mixed solvent with a volume ratio of 50:50 was added, ultrasonicated for 30 min, three times, cooled to room temperature, and cyclohexane with a volume ratio of 50:50 was added Dilute to volume with hexane-ethyl acetate mixed solvent. The extract was transferred to a centrifuge tube, centrifuged at 10,000 r / min for 10 min at 5°C, and the supernatant was filtered with a 0.45 μm membrane filter, and the filtrate was transferred to a GPC sample bottle.
[0042] (3) GPC purification treatment: The GPC detection wavelength was 254 nm, the mobile phase was a cyclohexane-ethyl acetate mixed solvent wi...
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