Process for industrial production of hydrolyzed wheat protein

A wheat hydrolyzed protein and process technology, which is applied in protein food processing, protein food ingredients, food science, etc., can solve the problems of single hydrolysis process, non-modification, and singleness, and achieve low free amino acid content, low nutrient loss, and production The effect of simple process

Inactive Publication Date: 2013-02-06
温志明
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] From the above research reports, it can be seen that most of the studies on enzymatic modification at the present stage use a single enzyme preparation an

Method used

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  • Process for industrial production of hydrolyzed wheat protein
  • Process for industrial production of hydrolyzed wheat protein
  • Process for industrial production of hydrolyzed wheat protein

Examples

Experimental program
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Example Embodiment

[0035] Example 1

[0036] 1) Add 10,000 kg of water to the reactor, heat it to 55°C, first add alkaline protease to the reactor at the ratio of 20 U / g wheat gluten powder, stir well, and then add 1000 kg to the reactor. For wheat gluten protein powder, add 1mol / L sodium hydroxide to the reaction system at the same time, adjust the pH of the system to 9.5, and stop adding sodium hydroxide after 15 minutes of reaction;

[0037] 2) When the pH value of the system is 8.5, trypsin is added to the system in an amount of 55 U / g wheat gluten powder, hydrolyzed for 60 min at a temperature of 50°C;

[0038] 3) Adjust the pH value to 8.0, add 0.8 kg of complex enzyme preparation to the system, hydrolyze for 3 h at a temperature of 60 ℃, the complex enzyme preparation is papain, soybean peptide hydrolase, flavor protease and enzyme stabilizer at a ratio of 1: 1.5 : 0.08: 0.01 weight ratio compound;

[0039] 4) Inactivate the enzyme at 95°C for 5 minutes, then filter with a 100 mesh filter;

[004...

Example Embodiment

[0041] Example 2

[0042] 1) Add 10,000 kg of water to the reactor, heat it to 60°C, first add alkaline protease to the reactor at the ratio of 30 U / g wheat gluten protein powder, stir well, and then add 2000 kg to the reactor. For wheat gluten protein powder, add 2mol / L potassium hydroxide to the reaction system at the same time, adjust the pH of the system to 10, and stop adding potassium hydroxide after 20 minutes of reaction;

[0043] 2) When the pH of the system is 9.0, trypsin is added to the system, the amount of which is 40 U / g wheat gluten powder, and the hydrolysis is carried out at a temperature of 55°C for 35 min;

[0044] 3) Adjust the pH value to 8.0, add 1.8 kg of complex enzyme preparation to the system, and hydrolyze it for 4 hours at a temperature of 60°C. The hydrolysis complex enzyme preparation is papain, soybean peptide hydrolase, flavor protease and enzyme stabilizer at a rate of 1 : 2: 0.12: 0.02 weight ratio compound;

[0045] 4) Inactivate the enzyme at 95°C...

Example Embodiment

[0047] Example 3

[0048] Add 10,000 kg of water to the reactor, heat it to 55°C, first add alkaline protease to the reactor at the ratio of 40 U / g wheat gluten protein powder, stir well, and then add 2500 kg of wheat gluten to the reactor For protein powder, add 4mol / L disodium hydrogen phosphate to the reaction system at the same time, adjust the pH of the system to 9, and stop adding disodium hydrogen phosphate after 25 minutes of reaction;

[0049] 2) When the pH value of the system is at 8.5, add trypsin to the system at an amount of 60 U / g wheat gluten powder, and hydrolyze it for 60 min at a temperature of 50°C;

[0050] 3) Adjust the pH value to 7.5, add 2.3 kg of complex enzyme preparation to the system, hydrolyze it for 3 hours at a temperature of 60 ℃, the hydrolysis complex enzyme preparation is papain, soybean peptide hydrolase, flavor protease and enzyme stabilizer : 2.2: 0.18: 0.03 weight ratio compound;

[0051] 4) Inactivate the enzyme at 100°C for 5 minutes, then fi...

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Abstract

The invention discloses a process for industrial production of hydrolyzed wheat protein. The hydrolyzed wheat protein is prepared by the following steps of: a, adding water into a reaction kettle, adding alkaline protease into the reaction kettle in a proportion of 20-40U/g wheat gluten protein powder, stirring to be uniform, adding the wheat gluten protein powder into the reaction kettle while alkaline is added to a reaction system to regulate pH value of the system to be 9-10.0, and stopping addition of the alkaline after reaction is carried out for 5-25min; b, when the pH value of the system is 8.5-9.0, adding trypsin into the system, and hydrolyzing for 30-80min at the temperature of 40-65 DEG C, wherein the added amount of the trypsin is 35-60U/g wheat gluten protein powder; c, regulating the pH value to be 7.5-8.0, adding complex enzyme preparation accounting for 0.08-0.5% of the wheat gluten protein powder, and hydrolyzing 1-6h at the temperature of 25-60 DEG C; and d, after hydrolyzing is completed, deactivating enzymatic hydrolysate, then filtering, and finally carrying out spray drying on filtrate. The process for industrial production of the hydrolyzed wheat protein disclosed by the invention has the advantages that protein digestibility is high, the hydrolyzed wheat protein is easy to absorb, the production process is simple and the production cycle is short.

Description

technical field [0001] The invention relates to a processing method of a protein additive for feed, in particular to a process for the industrial production of wheat hydrolyzed protein, which belongs to the comprehensive development and application field of wheat deep processing. Background technique [0002] Wheat gluten protein powder, also known as gluten powder, is a by-product of the process of producing wheat starch in industry. It is a powder product mainly made by washing, separating and drying. It is a cheap, high-quality, pure natural plant protein. , its protein content is as high as 72%-85%, containing 15 kinds of amino acids necessary for the human body, of which the glutamine content is as high as 30%, and the proline content is about 15%. It is rich in nutrients and has important physiologically active functions. Wheat gluten protein Powder is widely used and has great application prospects in the field of food industry. [0003] Wheat gluten is mainly compos...

Claims

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Application Information

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IPC IPC(8): A23J3/34
Inventor 温志明唐波
Owner 温志明
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