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Apatite/keratin composite scaffold and preparation method thereof

A composite scaffold and keratin technology, which is applied in the field of apatite/keratin composite scaffold and its preparation, can solve the problems of weakening the biological properties of keratin, and achieve the advantages of guiding bone tissue regeneration, easy operation and realization, and preparation method. simple effect

Active Publication Date: 2013-02-06
THE HONG KONG POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this way, calcium phosphate coats the surface of keratin, but at the same time it also covers and weakens the biological properties of keratin

Method used

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  • Apatite/keratin composite scaffold and preparation method thereof
  • Apatite/keratin composite scaffold and preparation method thereof
  • Apatite/keratin composite scaffold and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0037] as Ca(OH) 2 (99.995%), NaH 2 PO 4 (99.99%) and wool fibers were used as starting materials. 100ml Ca(OH) 2 The solution (0.1mol / l) was mixed with 200ml keratin solution (0.25g / L) and kept for 20min. NaH was then slowly added dropwise with stirring 2 PO 4 (60ml, 0.1mol / l); Now the molar ratio of Ca / P in the solution is 1.67. The pH of the solution was adjusted to 7.0 by NaOH solution (0.1 mol / l). When the pH exceeds about 6.0, the solution becomes supersaturated and some precipitation occurs. After keeping the saturated solution for 24 h, a precipitate of apatite-keratin complex was deposited at the bottom of the bottle. Then the precipitate was transferred to filter paper and repeatedly washed with pure water on the filter paper, and then freeze-dried at -60°C under vacuum to obtain an apatite / keratin composite scaffold. According to the above ingredients, the nominal weight ratio of apatite / keratin in the final apatite / keratin composite scaffold is 80 / 20 (wt / w...

Embodiment 2

[0052] CaCl 2 (99.995%), KH 2 PO 4 (99.99%) and wool fibers were used as starting materials. 150ml CaCl 2 The solution (0.01mol / l) was mixed with 200ml keratin solution (0.1g / L) and kept for 20min. NaH was then slowly added dropwise with stirring 2 PO 4 (100ml, 0.01mol / l); Now the molar ratio of Ca / P in the solution is set to 1.5. The pH of the solution was adjusted to 9.0 by NaOH solution (0.1 mol / l). When the pH exceeds about 6.0, the solution becomes supersaturated and some precipitation occurs. After keeping the saturated solution for 24 h, a precipitate of apatite / keratin complex was deposited at the bottom of the bottle. Then the precipitate was transferred to filter paper and repeatedly washed with pure water on the filter paper, and then freeze-dried at -60°C under vacuum to obtain an apatite / keratin composite scaffold. According to the above ingredients, the nominal weight ratio of apatite / keratin in the final apatite / keratin composite scaffold is 50 / 50 (wt / w...

Embodiment 3

[0054] Ca(OH) 2 (99.995%), NaH 2 PO 4 (99.99%) and wool fibers were used as starting materials. 100ml Ca(OH) 2 The solution (1.0mol / l) was mixed with 100ml keratin solution (10g / L) and maintained for 20min. NaH was then slowly added dropwise with stirring 2 PO 4(50ml, 1.0mol / l); At this moment, the molar ratio of Ca / P in the solution is set to 2.0. The pH of the solution was adjusted to 8.0 by NaOH solution (0.1 mol / l). When the pH exceeds about 6.0, the solution becomes supersaturated and some precipitation occurs. After keeping the saturated solution for 24 h, a precipitate of apatite / keratin complex was deposited at the bottom of the bottle. Subsequently, the solid-liquid separation of the precipitate was carried out, and the precipitate was redispersed into pure water. After repeated several times, the precipitate was transferred to filter paper, and then freeze-dried at -60° C. under vacuum to obtain an apatite / keratin composite scaffold. According to the above i...

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Abstract

The invention discloses an apatite / keratin composite scaffold and a preparation method thereof. The preparation method includes: precipitating apatite and keratin in aqueous solution to obtain precipitation of an apatite and keratin composition, flushing, filtering and freezing to dry the precipitation so as to form the apatite / keratin composite scaffold. The apatite / keratin composite scaffold has excellent biological property, such as biological computability, cellular affinity, biodegradability and morphological stability. Owning to the microstructure of the apatite / keratin composite scaffold, good attachment and multiplication of osteoblasts on the scaffold can be improved, guiding for regeneration of bone tissues is facilitated, and ultimate biodegradation is formed along with generation of new bone tissue. The preparation method is easy, and the apatite / keratin composite scaffold is prepared from raw materials in wide source, and is a novel artificial bone material with excellent bone induction performance and wide application prospect.

Description

technical field [0001] The invention relates to a bionic technique for guiding bone regeneration, and more particularly relates to an apatite / keratin composite scaffold and a preparation method thereof. Background technique [0002] In recent years, tissue engineering has emerged as the best solution for reconstructing defective tissue in the fields of medicine and engineering. One of the best areas of development is guided bone regeneration or guided tissue regeneration. [0003] It is currently known that hydroxyapatite (HA) is a major inorganic fraction constituting vertebrate hard tissues. In recent years, HA has attracted considerable attention in the field of bone tissue regeneration due to its highly optimized mechanical properties, biocompatibility, osteoconductivity, and bioactivity. However, the clinical application of HA is greatly limited due to its fragility, insufficient mechanical toughness, and easy migration of HA particles from the transplantation site. ...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/12
Inventor 李翼李加深胡军岩麦福达张晶
Owner THE HONG KONG POLYTECHNIC UNIV
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