Method for preparing immobilized enzyme on surface of polymer base material
A technology for immobilizing enzymes on the surface of substrates, applied in the directions of immobilization on/in organic carriers, textiles and papermaking, fiber processing, etc. Enzyme activity, improved mechanical strength, mild reaction conditions
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Example Embodiment
[0018] Example 1:
[0019] The LDPE film was extracted with acetone (the washing solution was extracted) for 72 hours and dried at room temperature. Cover the BOPP film on the LDPE film, take 50μL of isopropyl thioxanthone acetone solution (concentration 0.5M) and inject it between the double-layer polymers, and then use two quartz plates to compact the double-layer polymer. The propyl thioxanthone acetone solution is evenly distributed between the double-layer polymers to form a sandwich structure, and is moved into the high-pressure mercury lamp irradiation device (the light intensity at the wavelength of 254nm is 9000μW / cm 2 ) Irradiate at room temperature for 180 seconds. The LDPE film that has introduced dormant groups that can continue to initiate free radical polymerization was extracted with acetone for 1 hour to remove the isopropyl thioxanthone adsorbed on the surface, and dried at room temperature. Dissolve polyethylene glycol diacrylate (molecular weight 575) and glu...
Example Embodiment
[0023] Example 2
[0024] The LDPE film was extracted with acetone (the washing solution was extracted) for 72 hours and dried at room temperature. Cover the BOPP film on the LDPE film, take 20μL of isopropyl thioxanthone acetone solution (concentration of 0.3M) and inject it between the two-layer polymer, and then use two quartz plates to compact the two-layer polymer. The propyl thioxanthone acetone solution is evenly distributed between the double-layer polymers to form a sandwich structure, and is moved into the medium pressure mercury lamp irradiation device (the light intensity at the wavelength of 254nm is 5000μW / cm 2 ) Irradiate at room temperature for 150 seconds. The LDPE film that has introduced dormant groups that can continue to initiate free radical polymerization was extracted with acetone for 1 hour to remove the isopropyl thioxanthone adsorbed on the surface, and dried at room temperature. Dissolve polyethylene glycol diacrylate (molecular weight 575), acrylamid...
Example Embodiment
[0026] Example 3:
[0027] The LDPE film was extracted with acetone (the washing solution was extracted) for 72 hours and dried at room temperature. Cover the BOPP film on the LDPE film, take 30 μL of isopropyl thioxanthone acetone solution (concentration 0.5M) and inject it between the double-layer polymers, and then use two quartz plates to compact the double-layer polymer. The propyl thioxanthone acetone solution is evenly distributed between the double-layer polymers to form a sandwich structure, and is moved into the medium pressure mercury lamp irradiation device (the light intensity at the wavelength of 254nm is 5000μW / cm 2 ) Irradiate at room temperature for 200 seconds. The LDPE film that has introduced dormant groups that can continue to initiate free radical polymerization was extracted with acetone for 1 hour to remove the isopropyl thioxanthone adsorbed on the surface, and dried at room temperature. Dissolve polyethylene glycol diacrylate (molecular weight 575), hyd...
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