Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Glutamic acid preparation method

A technology of glutamic acid and bacterial strains, which is applied in the field of glutamic acid preparation, can solve the problems of inability to obtain glutamic acid, consume nutrient energy and self-growth transformation, and achieve the effect of shortening the fermentation cycle and increasing the acid production rate

Inactive Publication Date: 2013-02-13
SHANGHAI GUANSHENGYUAN TIANCHU FLAVORING
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The strains used in domestic glutamic acid fermentation technology are transformed into normal fermentation tanks, so the basic strains in the early stage of fermentation are consuming nutritional energy for self-growth transformation, and glutamic acid cannot be obtained at this stage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Using multi-level strains, the following process is carried out: slant strains → first-level shake flask seeds → second-level seed tank expansion cultivation (vegetative cells) → third-level seed tanks (first-level transformation cell culture, ultra-sub-moderate improvement process) → Fermentation culture (secondary transformation in fermenter, high biotin Tween-60 process).

Embodiment 2

[0018] 1. Obtain the transformed strain FM strain through super sub-appropriate and high biotin Tween-60 technology

[0019] 2. The strain was put into a 150L small-scale fermenter for experiments, and the results of glutamic acid production rate of 158mg / L and sugar-acid conversion rate of 61% were obtained, which have exceeded the domestic average data of glutamic acid production rate of 150mg / L

[0020] 3. The 150L fermentation tank used molasses as a carbon source to ferment successfully, and the acid production of glutamic acid was 160mg / L.

[0021] 4. The actual application of 250KL fermenter has been successful. The acid production from glutamic acid is 180mg / L, and the sugar-acid conversion rate is 63%, which is close to the advanced level of Ajinomoto's 183mg / L, which is ahead of the domestic market.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a glutamic acid preparation method. The preparation method which adopts comprises the following steps: inoculating bacterial strains into an inclined surface; carrying out primary shake flask seed culture; utilizing a secondary seed tank to carry out the enlarged culture of nutrition cells; utilizing a tertiary seed tank to carry out primary transition cell culture; carrying out fermentation culture through using a super-suboptimal improving technology; carrying out secondary transition in a fermentation tank; and carrying out a high-biotin tween-60 technology. The method has the advantages of effective shortening of the fermentation time, acid production improvement, discharge reduction, and generation of great influences to the improvement of economic benefits, and social benefits comprising environmental protection and the like.

Description

technical field [0001] The present invention specifically relates to a preparation method of glutamic acid. Background technique [0002] Glutamic acid is an acidic amino acid. The molecule contains two carboxyl groups, and its chemical name is α-aminoglutaric acid. Glutamic acid was discovered by Li Suxun in 1856. It is a colorless crystal with umami taste, slightly soluble in water, but soluble in hydrochloric acid solution, with an isoelectric point of 3.22. A large amount exists in cereal protein, and the content in animal brain is also more. Glutamic acid plays an important role in the protein metabolism process in organisms, and participates in many important chemical reactions in animals, plants and microorganisms. [0003] Medically, glutamate is mainly used to treat hepatic coma, and is also used to improve children's intellectual development. In the food industry, monosodium glutamate is a commonly used freshness enhancer for instruments, and its main component...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P13/14
Inventor 吴坚
Owner SHANGHAI GUANSHENGYUAN TIANCHU FLAVORING
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com