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L-methionine preparation method

A technology of methionine and acylated methionine, applied in the direction of fermentation, can solve the problems of high requirements and large investment, and achieve the effects of improving product yield, reducing production cost and improving production environment

Inactive Publication Date: 2013-02-13
NINGBO YUANFA BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chemical synthesis method has higher requirements on the process and equipment, and the investment is large.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation method of L-methionine of the present invention, this method comprises the steps:

[0025] S1. Dissolve DL-methionine with liquid caustic soda, add acetic acid, add aminoacylase after acidity adjustment, and acylate DL-methionine to generate acylated methionine.

[0026] The above-mentioned liquid caustic soda is a NaOH solution with a relatively dilute concentration, for example, a NaOH solution with a mass percentage of NaOH of 30-35%. The mass ratio of DL-methionine to NaOH satisfies 1:0.6.

[0027] Then, add acetic acid to the above liquid caustic soda dissolved with DL-methionine to neutralize the NaOH solution until the pH value is 7.0 to obtain a neutral solution. Acetic acid can adopt acetic acid solution, also can adopt pure acetic acid namely the acetic acid that content is (or higher than) 99%.

[0028] Add aminoacylase to neutral solution for reaction, DL-methionine generates acylated methionine under the action of aminoacylase.

[0029] S...

Embodiment 2

[0038] S1. Dissolve DL-methionine in 33% NaOH solution, and make the mass ratio of DL-methionine to liquid caustic soda 1:0.8. Then add 99% acetic acid to make the whole solution pH=7.0. After adjusting the pH, aminoacylase is added to acylate DL-methionine to generate acylated methionine.

[0039] S2. Add common mold to the acylated methionine solution, and ferment at 37° C. for 16 hours to produce a mixture of L-methionine and DL-methionine.

[0040] S3. Add hydrochloric acid to the above mixture to make the pH of the mixture PH=2.0, and then perform centrifugation on the mixture after adjusting the pH to obtain a centrifuge whose main component is DL-methionine.

[0041] Concentrate the centrifuged liquid into a paste, and then add liquid caustic soda to the concentrate to make the pH=7.0. Then add invertase to it to split DL-methionine into L-methionine.

[0042] The conversion process is carried out at a constant temperature of 37-39° C. for 40 hours, and then the conv...

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Abstract

The invention discloses an L-methionine preparation method, belongs to the chemical synthesis field, and solves the problems that a biological enzyme resolution method is low in economic indicator and large in pollution discharge, a traditional fermentation method is low in process yield and high in cost, and a chemical synthesis method is high in process and equipment requirements and large in investment. The preparation method includes the steps of obtaining acylated methionine due to acylation reaction of DL-methionine, adding common molds to ferment, generating mixture of L-methionine and the DL-methionine, adding hydrochloric acid for concentration by centrifuging, obtaining rough products after adding liquid caustic and invertase to concentrate and crystallize, and obtaining the L-methionine after decolourization and purification of the rough products. The L-methionine preparation method not only greatly improves product yield, but also shortens an operation cycle, improves original production environment, lowers production cost, and particularly suitable for wide production of the L-methionine.

Description

technical field [0001] The invention belongs to the field of chemical synthesis and relates to a method for preparing L-methionine, in particular to a method for preparing L-methionine by fermentation. Background technique [0002] L-methionine, also known as L-formic acid or L-methionine, English name L-Methionine, is a white flaky crystal or powder, soluble in water, warm dilute methanol, alkaline Solution and dilute inorganic acid, hardly soluble in ethanol, almost insoluble in ether. The molecular formula for L-methionine is C 5 h 11 NO 2 S, the structural formula is CH 3 -S-CH 2 -CH 2 -CH(NH 2 )COOH, the molecular weight is 149.21. [0003] Amino acids are divided into three types: D-type, L-type and DL-type. Generally, only the L-type amino acid can be used by the human body, and the D-type methionine (D-methionine) can be converted into L-type methionine (L-methionine) in the body. Used. [0004] L-methionine is widely used in medicine, food, biochemical rea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P41/00C12P13/12
Inventor 王裕祥
Owner NINGBO YUANFA BIOENG
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