Enrichment culture method of salt-tolerant efficient nitrosobacteria
A technology for the enrichment and cultivation of nitrosating bacteria, applied in the field of microorganisms, can solve the problems that nitrosating bacteria are not suitable for high-concentration salt environments, and achieve good economic and environmental benefits, good activity, and high concentration
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Embodiment 1
[0042] The first stage, the enrichment culture of nitrosifying bacteria resistant to high concentrations of ammonia nitrogen:
[0043] The activated sludge is taken from the nitrosation test device of the sewage treatment plant, and the activated sludge is inoculated into the aeration tank of the SBR reactor at a concentration of 2g / L, and the enriched culture medium is added. The enriched culture medium is composed of: NH 4 + -N(NH 4 HCO 3 ) 150mg / L, the increasing gradient of ammonia nitrogen concentration is 100mg / L; Fe 2+ (FeSO 4 ·7H 2 O) 10mg / L; Mg 2+ (MgSO 4 ·7H 2 O) 15mg / L; P (KH 2 PO 4 ) 60mg / L; Add sodium bicarbonate according to the carbon-nitrogen ratio (0.5-20):1. During the enrichment and cultivation of nitrosating bacteria, an operation cycle is 24 hours, including: water intake for 15 minutes, aeration for 20 hours, sedimentation for 2 hours, drainage for 1 hour, and the rest of the idle period; the dissolved oxygen value in the reactor is controlled a...
Embodiment 2
[0047] The enrichment culture process of the ammonia-nitrogen-resistant nitrosifying bacteria group of the first stage is different from embodiment 1: with 1g / L concentration, activated sludge is inoculated in the cascade tank of SBR reactor; 4 + -N(NH 4 HCO 3 ) 100mg / L; the increasing gradient of ammonia nitrogen concentration is 50mg / L; the dissolved oxygen value in the reactor is controlled at 1.0mg / L, and the pH is controlled at 6.5; the enrichment culture period of an ammonia nitrogen concentration is 3d; other conditions are the same as in Example 1 , the ammonia nitrogen concentration at the end reached 800mg / L.
[0048] The salt-tolerant domestication process of the high-concentration ammonium nitrogen-resistant nitrosifying bacteria group in the second stage is different from that of Example 1 in that: during the domestication process, the final concentrations of sodium chloride and sodium sulfate in the simulated wastewater were 6.0 g / L and 14.0 g, respectively. / ...
Embodiment 3
[0050] The enrichment culture process of the ammonia-nitrogen-resistant nitrosifying bacteria group of the first stage is different from embodiment 1: with 1.5g / L concentration, activated sludge is inoculated in the cascading pond of SBR reactor; Dissolved oxygen value in the reactor The pH is controlled at 6.5 mg / L, and the pH is controlled at 9.0; the enrichment culture period for one ammonia nitrogen concentration is 4 days; other conditions are the same as in Example 1, and the ammonia nitrogen concentration at the end reaches 850 mg / L.
[0051] The salt-tolerant acclimation process of the high-concentration ammonium nitrogen-resistant nitrosifying bacteria group in the second stage is different from Example 1 in that: in the acclimation process, the concentrations of sodium chloride and sodium sulfate in the simulated wastewater are respectively 2.5g / L, 3.5g / L The concentration of L increased gradually, and the final concentrations were 6.5g / L and 13.5g / L respectively. Th...
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