Anti-tumor extract, preparation method and application of bean root

An anti-tumor and northern bean root technology, applied in anti-tumor drugs, drug combinations, pharmaceutical formulations, etc., can solve problems such as complicated operations and residual organic reagents, and achieve the goal of ensuring purity, easy preparation, and avoiding the destruction of ingredients. Effect

Inactive Publication Date: 2018-02-23
HEILONGJIANG UNIV
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Problems solved by technology

[0002]The dried rhizome of the root system of the northern bean root system, Pueraria chinensis, the current active ingredient extraction process of the root of the northern bean adopts the conventional extraction method of alkaloids: that is, acid extraction and alkali extraction Precipitation combined with alkalization and organic solvent extraction, this extraction method has organic reagent residues, and the operation is cumbersome

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  • Anti-tumor extract, preparation method and application of bean root
  • Anti-tumor extract, preparation method and application of bean root

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A preparation method for the anti-tumor extract of bean root, the method comprises the steps of:

[0039] (1) Soaking and extraction: take the root of the northern bean root and soak it in 75% to 95% ethanol solution of 8 to 10 times the weight for 24 hours, then extract it three times with an ultrasonic extractor, and fully mix the three extracts;

[0040] (2) Concentration, dispersion, and centrifugation: After the mixed extract is concentrated under reduced pressure until it has no alcohol smell, then the concentrated solution and water are diluted at a volume ratio of 1:1, and centrifugal separation is performed;

[0041] (3) Use macroporous adsorption resin: take the centrifuged supernatant and inject it into the processed AB-8 macroporous adsorption resin, the weight ratio of supernatant to resin is 1:2;

[0042] (4) Elution: The adsorption resin is eluted at a flow rate of 1 column bed volume / hour, followed by elution with 3 to 5 column bed volumes of distilled w...

Embodiment 2

[0045] An anti-tumor extract of bean root, characterized in that it is prepared by the following method:

[0046] 1) Soaking and extraction: take the root of the northern bean root and soak it in 75% to 95% ethanol solution of 8 to 10 times the weight for 24 hours, then extract it three times with an ultrasonic extractor, and fully mix the three extracts;

[0047] (2) Concentration, dispersion, and centrifugation: After the mixed extract is concentrated under reduced pressure until it has no alcohol smell, then the concentrated solution and water are diluted at a volume ratio of 1:1, and centrifugal separation is performed;

[0048] (3) Use macroporous adsorption resin: take the centrifuged supernatant and inject it into the processed AB-8 macroporous adsorption resin, the weight ratio of supernatant to resin is 1:2;

[0049] (4) Elution: The adsorption resin is eluted at a flow rate of 1 column bed volume / hour, followed by elution with 3 to 5 column bed volumes of distilled w...

Embodiment 3

[0052] The application of an anti-tumor extract of bean root in the preparation of medicines in the field of anti-tumor.

[0053] The activity test of inhibiting human breast cancer cell MCF-7 is:

[0054] Cell culture:

[0055] Human breast cancer cell MCF-7 was cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum, L-glutamine, 100 U / ml penicillin and 100 mg / ml streptomycin, and the cells were placed at 37 ºC for 5 %CO 2 Cultured in an incubator with saturated humidity, incubated for 2-3 days and then digested and passaged with 0.25% trypsin, and cells in logarithmic growth phase were selected for subsequent experiments.

[0056] Cytotoxicity Assay:

[0057] 10 batches of the anti-tumor extracts prepared according to the above-mentioned preparation process were diluted with culture medium to different concentrations, filtered through a 0.22 mm microporous membrane, and the resulting filtrate was used for tumor cell proliferation inhibition experiments. In...

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Abstract

The invention relates to an anti-tumor extract of bean root, its preparation method and application. The current extraction process of the active components of bean root adopts the conventional extraction method of alkaloids: that is, acid extraction and alkali precipitation combined with organic solvent extraction after alkalization, and there are organic reagent residues in this extraction method. Steps: 1) Soaking extraction: Soak in 75%-95% ethanol solution for several hours and then ultrasonic extraction; (2) Concentration, dispersion, centrifugation: Concentrate the extract under reduced pressure until it has no alcohol smell, then dilute and centrifuge; (3) Use macroporous adsorption resin: take the centrifuged supernatant and inject it into the processed AB‑8 macroporous adsorption resin; (4) Elution: use the adsorption resin with a flow rate of 1 times the column bed volume/hour , followed by elution with 3 to 5 times the column bed volume of distilled water, 5 times the column bed volume of 10% ethanol solution and 4 times the column bed volume of 70% ethanol solution; (5) Prepare the anti-tumor extract : to collect 70% ethanol eluate, concentrate and dry to obtain dry solid. The invention is used in the field of medicine.

Description

Technical field: [0001] The invention relates to an anti-tumor extract of bean root, its preparation method and application. Background technique: [0002] The root system of the northern bean root is the dried rhizome of the plant Pueraria chinensis. The current extraction process of the active ingredients of the root of the northern bean adopts the conventional extraction method of alkaloids: that is, acid extraction, alkali precipitation, and organic solvent extraction after alkalization. There are organic reagents in this extraction method. residual, and the operation is cumbersome. Invention content: [0003] The purpose of the present invention is to adopt a preparation method of the anti-tumor extract of bean root. This method only uses water and ethanol, so that the extract has no organic residues, and can be made into effective traditional Chinese medicine suitable for clinical use with pharmaceutical excipients. site of antineoplastic drugs. [0004] Above-ment...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/59A61P35/00
Inventor 刘丽娟
Owner HEILONGJIANG UNIV
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