Biological preparation method for natural safe starch nano particles

A starch nano-biological preparation technology, applied in the field of nano-structured materials, can solve the problems of high toxicity, unfavorable large-scale industrial production, and increased cost of starch nano-particle preparation, and achieve the goal of shortening the production cycle, reducing requirements, and reducing the amount of use Effect

Active Publication Date: 2015-05-20
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The preparation of starch nanoparticles by the above method is relatively simple, but it is not conducive to large-scale industrial production. In the preparation process, toxic organic reagents and very expensive surfactants are used, which increases the preparation cost of starch nanoparticles. To some extent, its application is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Weigh 10g of waxy cornstarch, add it to 200mL of disodium hydrogen phosphate-citric acid buffer solution with a pH of 4.6, heat to make the starch milk completely gelatinized, cool to 60°C, this temperature is the optimum temperature for enzyme reaction, add Pullulanase, enzymatic hydrolysis for 4 hours, raise the temperature to inactivate the enzyme, centrifuge at low speed for 10 minutes while hot, remove the long-chain starch, and then slowly cool down, at this time, the short-chain amylose will aggregate with each other to form crystals, and aggregate at room temperature After 15 hours, centrifuge at a speed of 2000r / min for 20 minutes, take the supernatant and centrifuge at a speed of 5000r / min for 20 minutes, and vacuum freeze-dry the obtained precipitate to obtain starch nanoparticles of 20-500nm.

Embodiment 2

[0024] Weigh 20g of sweet potato starch, place it in 400mL of citric acid-sodium citrate buffer solution with a pH of 4.6, heat to make it gelatinize completely, then reduce the temperature of the water bath to 60°C, add isoamylase, react for 4 hours, and then Add amylase to react for 2 hours, raise the temperature to 90°C to make the enzyme activity completely disappear, centrifuge at low speed for 15 minutes while it is hot, remove the unreacted long-chain starch, then slowly cool down to 5°C, and let stand at this temperature After 10 hours, the short amylose was fully crystallized, and then centrifuged at a speed of 3000r / min for 20 minutes to obtain a precipitate, the precipitate was dissolved, and the solution was ultrasonically treated, and finally the solution after the vacuum freeze-drying process was obtained after 15 hours. ~800nm ​​starch nanoparticles.

Embodiment 3

[0026] Weigh 10g of potato starch, disperse it in 200mL of disodium hydrogen phosphate-citric acid buffer solution with a pH of 4.6, heat the starch milk to 100°C, after the starch is completely gelatinized, cool it to 60°C, add pullulanase and starch Enzyme, react for 5 hours, amylase can cut off the long-chain starch, pullulanase will remove the branched chain of amylopectin, then raise the temperature to 85°C, high temperature will kill the enzyme, and continue for 20 minutes at a speed of 2°C per minute Slowly lower the temperature, and let it stand for 12 hours when the temperature reaches 5°C. This process is the stage of short starch chain aggregation, and then centrifuge at a speed of 3000r / min for 20 minutes to obtain a precipitate, disperse the precipitate in acetone, and place the dispersion in Dry in a blast drying oven for 4 hours to obtain starch nanoparticles with a size of 20-600 nm.

[0027] The production of starch nanoparticles by biological enzyme method gr...

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Abstract

The invention discloses a biological preparation method for natural safe starch nano particles. The natural safe starch nano particles are prepared by gelatinization and recrystallization by using natural starch as a raw material, and the method belongs to the technical field of nano structure materials. The method comprises the following steps of: mixing the natural starch with water in a certain proportion to form starch milk; and performing gelatinization, debranching, low-speed centrifugation, recrystallization, differential speed centrifugation and vacuum freezing and drying to prepare the nanoscale starch crystalline. Toxic and harmful organic solvents and inorganic metal ions are not added during the preparation of the starch nano particles, and the starch nano particles are wide in sources of raw materials, biodegradable, good in biocompatibility, low in cost, and simple in production process, and industrial scale production of starch nano particles can be realized easily. The method has a special function in the fields of biological membrane preparation, medicine carriers, high-molecular polymer and the like.

Description

technical field [0001] The invention provides a method for preparing starch nanoparticles by a biological enzyme method, which belongs to the technical field of nanostructure materials. Background technique [0002] Nanotechnology is the most promising key technology in the world today. Nanomaterials play an important role and have broad application prospects in the fields of mechanics, electromagnetism, heat, optics and life sciences. [0003] In recent years, with the shortage of petroleum resources, the pollution of non-degradable plastics, the enhancement of people's awareness of environmental protection and their own health concerns, the research and development of new biodegradable materials has become a hot spot. Starch has the characteristics of wide sources, biodegradability, good biocompatibility and low cost. Nanotechnology using starch as raw material will become the focus of research in the next few years. Not only are natural raw materials degradable in nature...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/12C08L3/02
Inventor 孙庆杰熊柳李广华
Owner QINGDAO AGRI UNIV
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