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Multi-dimensional ion chromatographic analysis system

A technology of ion chromatography and analysis system, applied in the direction of analysis materials, material separation, measuring devices, etc., can solve problems such as insufficient resolution

Inactive Publication Date: 2013-03-13
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The technical problem to be solved by the present invention is to build a multi-dimensional ion chromatography analysis system, which can solve the complex samples of various non-polar substances such as hydrocarbons, long-chain fatty acids, proteins, and polar substances such as organic acids, amino acids, sugars and alcohols. The problem of insufficient resolution of the

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Embodiment 1

[0056] Instrument setup: ICS-3000 multifunctional chromatography device, including dual pump (DP) module, detector / chromatography (DC) module, autosampler (AS) module (Dionex, USA). Two analytical quaternary gradient pumps (i.e. Figure 4 pump 2 and pump 3 in ), the detector / chromatographic module places three six-way valves (the first one is the injection valve, the last two are switching valves), two quantitative loops, CRC cation trapping column (Dionex, 2.0×15mm), sugar analysis column CarboPac PA20 (4×250mm, Dionex) and guard column CarboPac PA20Guard (4×50mm, Dionex), amino acid analysis column Amino Pac PA10 (2×250mm, Dionex) and guard column Amino Pac PA10Guard ( 2×50mm, Dionex), two electrochemical detectors. The temperature was set at 30°C, and the amperometric detector used a gold working electrode, a pH-Ag / AgCl composite reference electrode, and a titanium counter electrode. P230 high pressure constant flow pump (ie Figure 4 Pump 1 in Dalian Yilite Instrument C...

Embodiment 2

[0068] Instrument setup: ICS-3000 multifunctional chromatography device, including dual pump (DP) module, detector / chromatography (DC) module, autosampler (AS) module (Dionex, USA). Two analytical quaternary gradient pumps (i.e. Figure 8 pump 2 and pump 3), detector / chromatographic module placed a six-way valve (injection valve), a ten-way valve (switching valve), two quantitative loops, cation exchange analysis column (customized, 2.1 × 150mm) , sugar analysis column CarboPac PA20 (4×250mm, Dionex) and guard column CarboPac PA20Guard (4×50mm, Dionex), electrochemical detector. The temperature was set at 30°C, and the amperometric detector used a gold working electrode, a pH-Ag / AgCl composite reference electrode, and a titanium counter electrode. P230 high pressure constant flow pump (ie Figure 8 Pump 1 in Dalian Yilite Instrument Co.) (see Figure 8 ).

[0069] Quantitative loop 1 is fixed on position ① and position ④ of the injection valve, positions ⑤ and position ⑥ o...

Embodiment 3

[0079] Instrument setup: ICS-3000 multifunctional chromatography device, including dual pump (DP) module, detector / chromatography (DC) module, autosampler (AS) module (Dionex, USA). The dual-pump module contains four analytical quaternary gradient pumps (i.e. Figure 12 Pumps 2, 3, 4 and 5 in the middle), the detector / chromatographic module places 3 six-way valves, 1 ten-way valve, 3 quantitative loops, cation trapping column, sugar analysis column CarboPac PA20 (4×250mm, Dionex) and guard column CarboPac PA20Guard (4×50mm, Dionex), amino acid analysis column Amino Pac PA10 (2×250mm, Dionex) and guard column Amino Pac PA10Guard (2×50mm, Dionex), organic acid analysis column AS11-HC ( 4×250mm, Dionex) and guard column AS11-HC Guard (4×50mm, Dionex), three electrochemical detectors. The temperature was set at 30°C, and the amperometric detector used a gold working electrode, a pH-Ag / AgCl composite reference electrode, and a titanium counter electrode. P230 high pressure consta...

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Abstract

The invention relates to a multi-dimensional ion chromatographic analysis system, belonging to the chemical field of analysis. To solve the problem that a conventional chromatographic technique is insufficient in separation of molecules such as various organic acid, amino acid, sugar and alcohol in complex samples, a set of multi-dimensional ion chromatographic analysis system is developed; the system comprises a plurality of pumps, one or more changeover valves and a plurality of detectors; by utilizing the difference of separation mechanisms of target components on chromatographic columns and the difference of retention time of the components, and through a plurality of chromatographic switchover enrichments and valve switchover time selection, ion chromatographic connected systems of two-dimension, three-dimension, four-dimension, five-dimension and higher can be established according to difference of target components to be separated, so that the coseparation analysis of water-soluble metabolite such as organic acid, amino acid, sugar and alcohol of complex samples is realized. By utilizing the multi-dimensional ion chromatographic analysis system, the complex sample can be rapidly, sufficiently and precisely separated and identified, and the mutual interference among the components is eliminated.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry in chemistry and can be widely used in the fields of bioenergy research, life science, food science, environment, petrochemical industry and the like. Background technique [0002] Amino acids and sugars are the main nitrogen and carbon sources for biological growth, and they play important physiological roles in cell metabolism. Simultaneously accurate and efficient separation and detection of these metabolites is of great significance for improving the productivity of energy components in cell systems and metabolomics research. In life science, food science, medicine, agronomy and other research fields, the analysis of organic acids, amino acids and related components such as sugars and sugar alcohols has become a hot spot. The traditional high-performance liquid chromatography (HPLC) detection requires derivatization, and the reagents are chemically toxic, and the operation is com...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 法芸高峻杨海燕杜鹃郑岳
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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