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Method for preparing dietary fiber of lotus seed shells by virtue of microwave-assisted enzymolysis

A dietary fiber and microwave-assisted technology, applied in the development of agricultural and sideline products, functional food additives and nutrition and health care, can solve the problems of low extraction efficiency, reduce resource waste and environmental pollution, reduce the burden of by-product treatment, and reduce costs Effect

Active Publication Date: 2013-03-20
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the effective components of lotus seed shells at home and abroad is still in its infancy, and the research on the dietary fiber of lotus seed shells is rarely involved, and most of them are based on conventional extraction methods, such as chemical reagents / enzymes, and the extraction efficiency is not high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 2 kg of dried lotus seed shells from Xianglian lotus, crush them through a 60-mesh sieve to obtain lotus seed shell powder; add 30 L of water, and stir slowly to obtain a mixture of lotus seed shell powder and water; adjust the pH of the mixture to 5.5, add Zhan lotus seed shell powder and water mixture Compound enzyme A with a mass fraction of 0.5% (mix α-amylase and glucoamylase at an enzyme activity ratio of 1:2.5, adjust the pH value to 4.5-6.5, and stir evenly to obtain compound enzyme A), microwave temperature 60 ℃, The power was 480 W, and the extraction time was 6×20 s (extraction 6 times, 20 s each time, with an interval of 2 min); after enzymatic hydrolysis treatment with compound enzyme A, the pH of the mixture was adjusted to 6.5, and the mass fraction of the system mixture was added to account for 0.5% compound enzyme B (mix papain and bromelain according to the enzyme activity ratio of 1:2, adjust the pH value to 4.5-6.5, stir well to obtain compound e...

Embodiment 2

[0023]Take 2 kg of dried lotus seed shells from Xianglian lotus, crush them through an 80-mesh sieve to obtain lotus seed shell powder, add 40 L of water, and stir slowly to obtain a mixture of lotus seed shell powder and water, adjust the pH of the mixture to 5.5, add Zhan lotus seed shell powder and water mixture Compound enzyme A with a mass fraction of 0.4% (mix α-amylase and glucoamylase at an enzyme activity ratio of 1:2.5, adjust the pH value to 4.5-6.5, and stir evenly to obtain compound enzyme A), microwave temperature 60 ℃, The power was 480 W, and the extraction time was 7×20 s (7 times, 20 s each time, with an interval of 2 min). After enzymatic hydrolysis treatment by compound enzyme A, adjust the pH of the mixture to 6.5, and add compound enzyme B (mix papain and bromelain at an enzyme activity ratio of 1:2, and adjust the pH value to 4.5-6.5, stir evenly to obtain compound enzyme B), microwave temperature 60 ℃, power 480 W, extraction time 7×20 s (extraction 7 t...

Embodiment 3

[0025] Take 2 kg of dried lotus seed shells from Xianglian lotus, crush them through a 60-mesh sieve to obtain lotus seed shell powder, add 30 L of water, and stir slowly to obtain a mixture of lotus seed shell powder and water, adjust the pH of the mixture to 5.5, add Zhan lotus seed shell powder and water mixture Compound enzyme A with a mass fraction of 0.6% (mix α-amylase and glucoamylase at an enzyme activity ratio of 2:3, adjust the pH value to 4.5-6.5, and stir evenly to obtain compound enzyme A), microwave temperature 60 °C, The power was 320 W, and the extraction time was 7×20 s (7 extractions, 20 s each time, with an interval of 2 min). After enzymatic hydrolysis treatment with compound enzyme A, adjust the pH of the mixture to 6.5, add compound enzyme B (mix papain and bromelain at an enzyme activity ratio of 1:2, and adjust the pH value to 4.5-6.5, stir evenly to obtain compound enzyme B), microwave temperature 60°C, power 320W, extraction time 7×20 s (extraction 7...

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PUM

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Abstract

The invention discloses a method for preparing dietary fiber of lotus seed shells by virtue of microwave-assisted enzymolysis. The method comprises the following steps of: drying the lotus seed shells, smashing and sieving the dried lotus seed shells to obtain solid powder of the lotus seed shells, adding water into the solid powder to obtain a mixture of the lotus seed shell powder and the water, performing microwave-assisted enzymolysis treatment on the mixture by complex enzyme A (namely alpha-amylase and saccharifying enzyme), performing microwave-assisted enzymolysis treatment on the mixture by complex enzyme B (namely papain and bromelain), inactivating the enzyme, performing static settlement on the mixture at a low temperature, filtering the mixture, and drying filtered residues, thus obtaining the dietary fiber of the lotus seed shells. The method can be used for achieving effective appreciating and efficient utilization of the lotus seed shells, namely by-products from processing of lotus seeds, and avoiding waste of resources and environment pollution caused by abandoning or incineration of the lotus seed shells (accounting for 35% of nelumbo nucifera gaertn in mass) in the prior art. The method is simple to implement and low in production cost, and the prepared product is high in purity and yield.

Description

technical field [0001] The invention belongs to the technical fields of development of agricultural and sideline products, functional food additives and nutrition and health care, and in particular relates to a method for preparing dietary fiber from lotus seed shells by microwave-assisted enzymolysis. Background technique [0002] Lotus (Nelumbo nucifera Gaertn) is a perennial aquatic herb and is a special product in my country. Lotus seeds are recognized as a nourishing product suitable for all ages. It is more suitable for middle-aged and elderly people with physical weakness, insomnia, loss of appetite and cancer patients. Hunan is the main production province of lotus seeds. As a by-product of lotus seed processing, lotus seed shells account for about 35% of the mass of lotus shells. As many as 25,000 tons of lotus seed shells are produced every year when processing lotus seeds. The main nutritional components are high in fiber content, followed by Protein, starch, tann...

Claims

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Application Information

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IPC IPC(8): A23L1/308
Inventor 王建辉刘永乐俞健王发祥李向红靳娜
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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