Preparation method for improving productivity of mitomycin C

A kind of technology of mitomycin and yield, applied in the field of preparation of improving mitomycin C yield

Active Publication Date: 2013-04-03
HUBEI HONCH PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when mitomycin C is produced industrially by fermentation and cultivation methods, the content of mitomycin C in the produced product usually only accounts for less than about 12%, and sometimes even only traces

Method used

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  • Preparation method for improving productivity of mitomycin C
  • Preparation method for improving productivity of mitomycin C
  • Preparation method for improving productivity of mitomycin C

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The mitomycin C-producing strain used in this example is Streptomyces capitis ATCC27422, and the culture medium can be a common medium suitable for cultivating actinomycetes. The actual slant medium used in this example consists of 1.0% starch , calcium carbonate 0.1%, potassium nitrate 0.1%, magnesium sulfate 0.05%, sodium chloride 0.05%, potassium dihydrogen phosphate 0.03%, asparagine 0.002%, bran 2.0%, agar 2.0%, the rest is water PH natural ( The above percentages are all percentages by mass). Sterilize at 120°C for 30 minutes, pave it into a solid slope, inoculate the bacteria on the slope, and cultivate it at 28±2°C for 8 days, inoculate the well-grown slope bacteria on the 120°C sterilized surface by digging blocks. In the 30-minute seed medium, the composition of the seed medium is 1% glucose, 3% starch, 2.0% bean cake powder, 0.5% corn steep liquor, 0.1% sodium chloride, 0.01% potassium dihydrogen phosphate, 0.2% corn oil, and the rest Adjust the pH of the wa...

Embodiment 2

[0024] The strain that produces Mitomycin C used in this example is Streptomyces capitis ATCC27422, and its slant medium consists of starch 1.0%, calcium carbonate 0.1%, potassium nitrate 0.1%, magnesium sulfate 0.05%, sodium chloride 0.05%, Potassium dihydrogen phosphate 0.03%, asparagine 0.002%, bran 2.0%, agar 2.0%, and the rest is water with natural pH. Sterilize at 120°C for 30 minutes, spread it into a solid slope, inoculate the bacteria on the slope, and cultivate it at 28±2°C for 6 days, inoculate the well-grown slope bacteria on the 120°C sterilized surface by digging blocks. In the 30-minute seed medium, the composition of the seed medium is 2% glucose, 1% starch, 1.5% bean cake powder, 0.5% corn steep liquor, 0.1% sodium chloride, 0.01% potassium dihydrogen phosphate, 0.2% corn oil, and the rest Adjust the pH of the water to 7.00. Rotate and shake at 28°C for 48 hours after inoculation, and then use as seeds for fermentation. Add citrulline, arginine composite pre...

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Abstract

The invention provides a preparation method for improving productivity of mitomycin C. The preparation method comprises the following steps: adding a composite precursor containing citrulline and arginine and HP-21 resin into a fermentation medium of the mitomycin C to prepare a dedicated fermentation medium; and culturing the head-shaped streptomyces generating mitomycin C respectively through a slant medium and a seed medium, then transferring to the prepared dedicated fermentation medium for culturing. Due to the action of the composite precursor containing citrulline and arginine, the content of mitomycin C in the effective compound generated by fermentation is greatly improved to 30-45 percent, so that the production cost of mitomycin C can be greatly reduced, as a result, the method for preparing mitomycin C has favorable industrial prospect.

Description

technical field [0001] The invention relates to a preparation method for improving the yield of mitomycin C, which belongs to the field of antibiotic microbial fermentation and cultivation. Background technique [0002] Mitomycin C belongs to the benzoquinone antibiotics and is a class of antibiotics with a unique structure and unique biological activity. Its molecular structure: [0003] [0004] Mitomycin C is a kind of benzoquinone compound produced by Streptomyces capitis. Mitomycin C not only has a broad-spectrum anti-tumor effect, but also has a good therapeutic effect on glaucoma, chronic dacryocystitis and other ophthalmic diseases. , has a good application prospect. But at present, when Mitomycin C is produced industrially by fermentation culture method, the content of Mitomycin C in the produced product usually only accounts for less than about 12%, sometimes even only traces. [0005] Therefore, it is an urgent technical problem to be solved in this field to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12R1/465
Inventor 陈庆源何福彪张文凯
Owner HUBEI HONCH PHARMA
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