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Biofuel production

一种组合物、发酵产品的技术，应用在生物燃料、微生物、基于微生物的方法等方向，能够解决增加成本等问题

Inactive Publication Date: 2013-04-10
STELLENBOSCH UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process involves large temperature changes (typically in the range of about 32-120°C), which uses a large amount of thermal energy, which will greatly increase the cost of the process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0295] Materials and methods

[0296] Strains and Media

[0297] All chemicals, media components and additives are of analytical grade standard. The genotypes and origins of the plasmids, yeast and bacterial strains used in the experiments are summarized in Table 1. Recombinant plasmids were constructed and amplified in Escherichia coli XL1-Blue. Bacterial strains were grown on rotating wheels in Terrific Broth or on LB agar (Luria-Bertani agar) at 37°C (Sambrook et al., 1989). Add ampicillin for selection of resistant bacteria to a final concentration of 100 μg ml -1 . Fungal strains were grown at 30°C on a rotary shaker set at 100 rpm. Aspergillus strains were grown in maltodextrin medium (5% maltodextrin, 0.6% NaNO 3 , 0.15% KH 2 PO 4 , 0.05% MgSO 4 , 0.05% KCl, and trace elements). Fungal strains were stored at 30°C in minimal medium (1% glucose, 0.6% NaNO 3 , 0.2% peptone, 0.15% KH 2 PO 4 , 0.1% yeast extract, 0.1% casamino acids, 0.05% MgSO 4 , 0.05% KCl, 2...

Embodiment 2

[0388] Embodiment 2: Design novel amylolytic yeast strain for the production strain and medium of industrial ethanol

[0389] All chemicals, media components and additives are of analytical grade standards. Recombinant plasmids were constructed and amplified in E. coli DH5α. Bacterial strains were grown in broth at 37°C on a turntable (Sambrook et al., 1989). Add ampicillin as a selection of resistant bacteria to a final concentration of 100 μg ml -1 . Saccharomyces cerevisiae strains can be grown in YPD medium (1% yeast extract, 2% peptone and 2% glucose) containing other growth factors and amino acids if necessary at 30 °C on a rotary shaker set at 100 rpm and selective complete medium (2% glucose and 0.17% yeast nitrogen source without amino acid).

[0390] DNA processing

[0391] Restriction enzyme digestion, electrophoresis, DNA ligation, transformation and DNA preparation from E. coli were performed using standard methods according to Sambrook et al. (1989). Purifi...

Embodiment 3

[0408] Example 3: Screening and development of an effective saccharification yeast strain for the production of industrial ethanol

[0409] Screening of Saccharomyces cerevisiae and non-Saccharomyces cerevisiae strains for the production of extracellular hydrolases

[0410] The media used in this study are shown in Table (a) below. All chemicals, media components and supplements were of analytical grade.

[0411] culture medium

reference or supplier

Edimburgh Minimal Medium (EMM)

Favaro et al., 2008

Minimal Media Yeast (MMY)

Favaro et al., 2008

Nutrient Agar (NA)

DIFCO

Wollum medium

Wollum et al., 1982

[0412] Table (a). Summary of media used in this study

[0413] Screening of Saccharomyces cerevisiae and non-Saccharomyces cerevisiae strains for the production of extracellular hydrolases

[0414] 220 S. cerevisiae strains and 180 non-S. cerevisiae isolates were screened for amylolytic activity. Saccharomyces...

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Abstract

The invention relates to the combination of the TLG1 glucoamylase from Thermomyces lanuginoses with: (i) the SFA1 alpha-amylase from Saccharomycopsis fibuligera; and / or (ii) the LKA alpha-amylase. The enzyme combinations may be expressed in a host cell (e.g. in Saccharomyces cerevisiae) or provided as an enzyme compostion. Methods for making the enzyme combinations of the invention are provided. The invention also relates to a yeast strain which exhibits amylolytic activity and promising fermentative abililties. Processes for producing a fermentation product (in particular alcohol) from stairch-containing material are described.

Description

technical field [0001] The present invention relates to the use of fungal enzymes and microbial systems for industrial processing, in particular for the microbial conversion of starch-containing materials, such as raw starch, to produce ethanol. [0002] All documents cited herein ("documents cited herein") and all cited documents or cited documents referred to herein, as well as sources from GenBank and other databases mentioned herein, are reproduced in their entirety Reference. [0003] No admission is made that any of the various documents cited herein are prior art to the present invention. Background technique [0004] Plant biomass is a carbon-neutral renewable resource and biomass conversion, specifically cellulosic feedstock conversion, has received a lot of attention as a clean alternative to petroleum due to its abundance and low cost. The current process for converting starch to bioethanol is well established, but with high energy costs, it is clear that a more...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/34C12P7/06C12P19/20C12N9/30C12P19/14C12R1/865
CPCC12N9/242C12N9/2428C12P7/06C12P19/14C12N9/2402Y02E50/10C12N15/81
Inventor 威廉·希伯·范齐尔塔尼亚·约斯特约翰·费迪南德·格根斯马林娜·萨伊曼洛伦佐·法瓦罗玛丽娜·巴萨利亚塞吉奥·卡塞拉
Owner STELLENBOSCH UNIVERSITY
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