Application of des-O-methyllasiodiplodin in preparation of medicament for treating kidney diseases
A technology of methylmadiprene and kidney disease, which is applied in the application field of de-O-methylmadiprene in the preparation of medicines for treating kidney disease, and can solve the problems of no research reports on the therapeutic effect of kidney disease , to achieve a good therapeutic effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0019] Embodiment 1: The pharmacological effect of des-O-methylmadiprene on nephrotic syndrome
[0020] 1. Experimental materials
[0021] Aminonucleoside puromycin was purchased from Sigma Company of the United States; SuperScript III RNase H-Reverse Transcriptase Kit was purchased from Invitrogen Company of the United States; goat anti-nephrin, podocin antibody, horseradish peroxidase-labeled anti-goat and mouse secondary antibodies were purchased from American Santa Cruz company.
[0022] 2. Experimental method
[0023] 1. Animal model preparation and experimental grouping: 40 male SD rats, weighing 80-100 grams. Randomly divided into 5 groups, normal control group, model group, de-O-methylmaidepredrine (DML) low-dose treatment group, de-O-methylmaudepredrine (DML) high-dose treatment group and de-O-methylmaudepredrine (DML) control group, 8 rats in each group (n=8). Normal control group: intraperitoneal injection of normal saline; model group, des-O-methylmaideprene lo...
Embodiment 2
[0038] Example 2 Pharmacological effects of des-O-methylmaudeprene (DML) on mesangial proliferative glomerulonephritis.
[0039] 1. Experimental materials
[0040] 40 healthy male SD rats, 4 weeks old, weighing about 160g, were purchased from the Experimental Animal Center of Jiangsu Province; complete Freund's adjuvant, FITC-labeled goat anti-rabbit IgG and Schiff reagent were purchased from Sigma Company of the United States, proliferating cell nuclear antigen ( PCNA) and monocyte chemoattractant-1 (MCP-1) monoclonal antibody were purchased from Sant Cruz Company in the United States, horseradish peroxidase-labeled goat anti-rabbit IgG, normal goat serum blocking solution, DAB chromogenic reagent were purchased from Beijing Zhongshan Biotechnology Company.
[0041] 2. Experimental method
[0042] 1. Preparation of anti-rat thymocyte antiserum (ATS): 4-week-old SD rats, take their thymus and prepare a certain concentration of single thymocyte suspension, mix with complete F...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com