Preparation method of pH and thermal dual-sensitive lipidosome loaded with magnetic carbon quantum dots on internal water phase

A technology of carbon quantum dots and liposomes, which is applied in the field of preparation of pH and thermal amphipathic liposomes, and achieves the effects of low cost and simple method

Inactive Publication Date: 2013-04-17
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there has not been a liposome modified on the surface of a pH- and heat-sensitive polymer, hydrothermally synthesized inner water phase-loaded carbon quantum dots / nano ferric oxide composite particles, and has fluorescence, magnetism, pH and heat sensitivity. Relevant Chinese patent reports on a new type of multifunctional liposome with sensibility

Method used

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  • Preparation method of pH and thermal dual-sensitive lipidosome loaded with magnetic carbon quantum dots on internal water phase
  • Preparation method of pH and thermal dual-sensitive lipidosome loaded with magnetic carbon quantum dots on internal water phase
  • Preparation method of pH and thermal dual-sensitive lipidosome loaded with magnetic carbon quantum dots on internal water phase

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Experimental program
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Embodiment 1

[0028] The structure of the pH and thermal dual-sensitive liposomes loaded with magnetic carbon quantum dots in the inner water phase is as follows: figure 1 As shown, the detailed preparation steps are as follows: under rapid magnetic stirring, the DMF solution dissolved with 1mol DDC was added dropwise to the 10mL DMF solution dissolved with 0.1mol PAA and 1mol DDA to form a mixed solution, and then reacted at 60°C After 24 hours, the polymer PAA-DDA with a PAA carboxyl grafting rate of 20% was obtained. Next, 90 mg NIPAM, 10 mg PAA-DDA and 5 mg AIBN were weighed and dissolved in 1,4-dioxane, and reacted at 70° C. for 12 h to obtain PNAD, a triblock copolymer of PNIPAM-PAA-DDA.

[0029]Dissolve 0.2mol ferric chloride, 0.1mol ferrous sulfate and 0.3mol thioglycolic acid in 100mL deionized water, adjust the pH of the mixture to 7.5, and react under magnetic stirring at 65°C to produce a large amount of black ferric oxide For nanoparticles, polyethylenimine is added dropwise t...

Embodiment 2

[0033] Under rapid magnetic stirring, the DMF solution dissolved with 1mol DDC was added dropwise to 10mL DMF solution dissolved with 0.1mol PAA and 1.5mol DDA to form a mixed solution, and then reacted at 70°C for 24h to obtain the PAA carboxyl graft ratio 30% polymer PAA-DDA. Next, 85mg NIPAM, 15mgPAA-DDA and 5mg AIBN were weighed and dissolved in 1,4-dioxane, and reacted at 70°C for 12h to obtain PNAD, a triblock copolymer of PNIPAM-PAA-DDA.

[0034] Dissolve 0.1mol ferric chloride, 0.05mol ferrous sulfate and 0.15mol thioglycolic acid in 100mL deionized water, adjust the pH of the mixture to 7.5, and react under magnetic stirring at 65°C to produce a large amount of black ferric oxide For nanoparticles, polyethylenimine is added dropwise to a mass fraction of 0.1%. Dissolve 2g of glucose with 10mL PEG-200 and transfer it to a microwave reactor, and react at a power of 540W for 5 minutes to prepare carbon quantum dots, which are then added dropwise to the magnetic particle...

Embodiment 3

[0037] Under rapid magnetic stirring, the DMF solution dissolved with 1mol DDC was added dropwise to the 10mL DMF solution dissolved with 0.1mol PAA and 2.0mol DDA to form a mixed solution, and then reacted at 70°C for 24h to obtain the PAA carboxyl graft ratio 40% polymer PAA-DDA. Next, 85 mg NIPAM, 15 mg PAA-DDA and 10 mg AIBN were weighed and dissolved in 1,4-dioxane, and reacted at 70° C. for 12 h to obtain PNAD, a triblock copolymer of PNIPAM-PAA-DDA.

[0038] Dissolve 0.1mol ferric chloride, 0.05mol ferrous sulfate and 0.15mol thioglycolic acid in 100mL deionized water, adjust the pH of the mixture to 7.5, and react under magnetic stirring at 65°C to produce a large amount of black ferric oxide For nanoparticles, polyethylenimine is added dropwise to a mass fraction of 0.1%. Dissolve 3g of glucose in 15mL PEG-200, transfer to microwave reactor, and react at 540W power for 5 minutes to prepare carbon quantum dots, which are then added dropwise to the magnetic particle so...

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Abstract

The invention relates to a preparation method of pH and thermal dual-sensitive lipidosome loaded with magnetic carbon quantum dots on an internal water phase, which concretely comprises the following steps of: 1) preparing nano ferroferric oxide by a co-precipitation method, carrying out surface modification by polyethyleneimine, preparing carbon quantum dots by a microwave pyrolysis method, and adding the carbon quantum dots into the modified magnetic particle solution to form the magnetic carbon quantum dots; and 2) loading the magnetic carbon quantum dots into the internal water phase of the lipidosome by a low-pressure film formation-high-pressure incubation method, and grafting the pH and heat sensitivity triblock copolymer on the surface of the lipidosome by hydrophobic effect to prepare the lipidosome modified by nano particles and polymer. Compared with the prior art, the preparation method is simple and lower in cost; the prepared product can be developed into a diagnosis therapeutic agent with functions of magnetic targeting, fluorescence imaging or tracing; and the medicine carrying system of pH and thermal controlled-release medicine has important reference value for developing other types of multifunctional medicine carriers.

Description

technical field [0001] The invention belongs to the technical field of biomaterials and nanomedicine, and in particular relates to a preparation method of pH- and heat-sensitive liposomes loaded with magnetic carbon quantum dots in an inner water phase. Background technique [0002] Liposome refers to a closed vesicle composed of phospholipids as a membrane material and additives. It has a bilayer structure similar to a biological membrane. It can encapsulate water-soluble and fat-soluble drugs in the capsule and become a new type of drug carrier. Phospholipids, the basic components of liposomes, are inherent components of organisms, can be degraded by biotransformation in vivo, and are non-toxic and immunogenic. In view of its advantages of improving drug stability, reducing drug dosage, reducing toxicity, alleviating immune response, delaying release, reducing in vivo digestion speed, and changing drug distribution in the body, liposomes are considered to be one of the mos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18A61K49/12A61K49/00A61K9/127A61K47/32
Inventor 万锕俊桂日军李慧丽金辉
Owner SHANGHAI JIAO TONG UNIV
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